目的 探讨珠子参总皂苷对人巨细胞病毒（HCMV）感染人胚肺成纤维细胞（MRC-5）炎症反应、凋亡的影响及其对miR-216a的调控作用。 方法 采用HCMV感染MRC-5细胞建立病毒感染模型，不同浓度（100 μg/mL、200 μg/mL、400 μg/mL）的珠子参总皂苷处理细胞，分别将miR-NC、miR-216a mimics转染至HCMV感染的MRC-5细胞，分别将anti-miR-NC、anti-miR-216a转染至HCMV感染的MRC-5细胞后加入珠子参总皂苷处理细胞；采用ELISA法检测TNF-α、IL-6水平，流式细胞术检测细胞凋亡率，qRT-PCR法检测miR-216a表达量。 结果 珠子参总皂苷可降低HCMV感染的MRC-5细胞凋亡率及TNF-α、IL-6的水平（P<0.05），而miR-216a的表达水平升高（P<0.05）；转染miR-216a mimics可降低凋亡率及TNF-α、IL-6的水平（P<0.05）；转染anti-miR-216a可降低珠子参总皂苷对HCMV感染的MRC-5细胞炎症反应及凋亡的作用。 结论 珠子参总皂苷可通过上调miR-216a的表达而抑制炎症反应及细胞凋亡从而减轻HCMV感染的MRC-5细胞损伤。
Objective To explore the effect of rhizome panacis majoris total saponin on the inflammatory response and apoptosis of human embryonic fibroblast (MRC-5) infected by human cytomegalovirus (HCMV) and its regulatory effect on miR-216a. Methods MRC-5 cells were infected with HCMV to establish a virus infection model. The cells were treated with panacis majoris rhizome total saponin at different concentrations (100, 200, 400 μg/mL). The miR-NC and miR-216a mimics were transfected into HCMV-infected MRC-5 cells. After transfecting anti-miR-NC and anti-miR-216a into HCMV-infected MRC-5 cells, rhizome panacis majoris total saponin was added to the cells. ELISA was used to detect the levels of TNF-α and IL-6. Flow cytometry was used to detect the apoptosis rate. qRT-PCR method was used to detect the expression of miR-216a. Results Rhizome panacis majoris total saponin could reduce the apoptosis rate and the levels of TNF-α and IL-6 in MRC-5 cells infected with HCMV (P<0.05), while increase the expression level of miR-216a (P<0.05). Transfection of miR-216a mimics could reduce the rate of apoptosis and the levels of TNF-α and IL-6 (P<0.05). Transfection of anti-miR-216a could reduce the effect of rhizome panacis majoris total saponin on the inflammation and apoptosis of MRC-5 cells infected with HCMV. Conclusions Rhizome panacis majoris total saponin can inhibit the inflammatory response and apoptosis by up-regulating the expression of miR-216a, thereby reducing the damage of MRC-5 cells infected by HCMV.
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