目的 探讨变应性鼻炎（allergic rhinitis，AR）病理过程中TLR-NF-κB信号传导通路的调节机制及核因子-κB（NF-κB）对嗜酸性粒细胞聚集的调节作用。 方法 50只大鼠随机分成A组（对照组）、B组（AR组）、C组（AR+PGN）、D组（AR+PGN+Pam3CSK4）、E组（AR+PGN+BEL），应用卵清蛋白建造AR模型，HE染色观察鼻黏膜形态改变并计数炎症细胞浸润数，ELISA法检测鼻腔清洗液IFN-γ、IL-4、嗜酸性粒细胞主要碱性蛋白（MBP）及IgE的含量，Real-Time PCR及Western blot法检测Toll样受体2（TLR2）、NF-κB的表达情况。 结果 B组变应性损伤明显，鼻黏膜嗜酸性粒细胞浸润数多于其他组，IL-4、MBP及IgE的表达较A组增高（P<0.05）；C组鼻黏膜以中性粒细胞浸润为主，IFN-γ、TLR2及NF-κB的表达较B组增高（P<0.05）；D、E组炎症细胞浸润数、IL-4、MBP及IgE的表达较B组降低（P<0.05）。 结论 调控TLR-NF-κB传导通路的表达即抑制NF-κB的过度表达，有利于调节变应性鼻炎病理过程中嗜酸性粒细胞的增值聚集。
Objective To experimentally investigate a potential mechanism of the TLR-NF-κB signaling pathway in the pathogenesis of allergic rhinitis (AR), and the role of NF-κB in eosinophil aggregation. Methods A total of 50 rats were randomly divided into five groups with A (control group), B (AR group), C (group of AR+PGN), D (group of AR+PGN+Pam3CSK4), and E (group of AR +PGN+BEL). AR model was established with ovalbumin. Hematoxylin and eosin (H&E) staining was used to detect the morphologic change of nasal mucosa and count the number of infiltrated inflammatory cell. The contents of IFN-γ, IL-4, MBP and IgE in nasal cleanser were detected by ELISA. Expression of NF-κB and TLR2 was measured by Real-Time polymerase chain reaction (PCR) and Western blot. Results Obvious allergic injury was observed in AR rats (B group). The number of infiltrated eosinophils in nasal mucosa in group B was higher than in other groups. The expressions of IL-4, MBP and IgE in group B were higher than in group A (P＜0.05). Neutrophil infiltration was predominant in nasal mucosa of group C, and the expression of IFN-γ, TLR2 and NF-κB was higher in group B (P＜0.05). Inflammatory cell infiltration, IL-4, MBP and IgE expression in group D and E were lower than in group B (P＜0.05). Conclusions Regulating the expression of TLR-NF-κB pathway and inhibiting the over-expression of NF-κB is beneficial to regulating the aggregation of eosinophils in the pathogenesis of allergic rhinitis.
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