少阳主骨方对食蟹猴自然退变膝关节骨性关节炎模型p16/Rb信号通路的影响

周鑫; 张磊; 扶世杰; 刘刚; 郭晓光; 汪国友

doi:10.13418/j.issn.1001-165x.2017.06.013

中国临床解剖学杂志 ›› 2017, Vol. 35 ›› Issue (6) : 655-660. DOI: 10.13418/j.issn.1001-165x.2017.06.013

实验研究

少阳主骨方对食蟹猴自然退变膝关节骨性关节炎模型p16/Rb信号通路的影响

周鑫，　张磊，　扶世杰，　刘刚，　郭晓光，　汪国友

作者信息 +

Effects of ShaoYangZhuGuFang on p16/Rb signaling pathway of naturally degenerative cynomolgus monkeys knee osteoarthritis model

ZHOU Xin, ZHANG Lei, FU Shi-jie, LIU Gang, GUO Xiao-guang, WANG Guo-you

Author information +

文章历史 +

摘要

目的　根据中医“少阳主骨”理论，研究少阳主骨方延缓关节软骨细胞衰老和退变的作用机制。方法　通过X线选取13只膝关节退变的老年食蟹猴作为动物模型，随机选取1只进行病理学观察，其余食蟹猴随机分为少阳主骨方组、氨糖美辛组、生理盐水组，每组4只，连续灌胃8周后处死所有食蟹猴，对关节软骨进行病理学观察，RT-qPCR、Western-blot检测关节软骨中p16、Rb基因与蛋白的表达。结果　老年食蟹猴KOA模型关节软骨病理改变符合KOA病理变化特点，三组食蟹猴Mankin评分少阳主骨方组(7.5±0.53)分、氨糖美辛组(7.75±0.71)分、生理盐水组(8.25±0.46)分，少阳主骨方组与生理盐水组间具有统计学差异（P<0.05）；p16基因与蛋白的表达少阳主骨方组<氨糖美辛组<生理盐水组，具有统计学差异（P<0.05）；Rb基因与蛋白的表达少阳主骨方组>氨糖美辛组>生理盐水组，具有统计学差异（P<0.05）。结论　少阳主骨方可以通过p16/Rb途径延缓关节软骨细胞衰老，从而延缓关节软骨退变。

Abstract

Objective　 The aim of this study was to investigate the mechanism of ShaoYangZhuGuFang(SYZGF) in delaying the cell senescence and degeneration of articular chondrocytes according to the theory of "ShaoYangZhuGu". Method 13 cynomolgus monkeys with degenerative knee joints were selected by X-ray. One of the monkeys was selected randomly for pathologic observation, and the other cynomolgus monkeys were randomly divided into SYZGF group, Ammonia Moxime group and Saline group. There were four cynomolgus monkeys in each group. All cynomolgus monkeys were executed after 8 consecutive weeks of intragastric administration. Pathological observation of articular cartilage was made and the expression of p16, Rb gene and protein in articular cartilage was detected by RT-qPCR and Western-blot technique.　Result　Articular cartilage pathological changes of elderly cynomolgus monkey KOA models was consistent with the characteristics of KOA pathological changes. Mankin scores of cynomolgus monkeys in three groups were as follows: 7.5 ± 0.53 in SYZGF group, 7.75 ± 0.71 in Ammonia Moxime group, 8.25 ± 0.46 in Saline group, and there was significant difference between SYZGF group and Saline group (P<0.05). The expression of p16 was lowest in SYZGF group, which in middle in Ammonia Moxime group, and highest in Saline group. There was significant difference in the three groups (P<0.05). The expression of Rb was highest in SYZGF group, middle in Ammonia Moxime group, and lowest in Saline group. There was significant difference in the three groups (P<0.05).　Conclusion　SYZGF could delay chondrocyte senescence by p16/Rb pathway and delay articular cartilage degeneration.

导出引用

周鑫,张磊,扶世杰,刘刚,郭晓光,汪国友. 少阳主骨方对食蟹猴自然退变膝关节骨性关节炎模型p16/Rb信号通路的影响[J]. 中国临床解剖学杂志. 2017, 35(6): 655-660 https://doi.org/10.13418/j.issn.1001-165x.2017.06.013

ZHOU Xin, ZHANG Lei, FU Shi-jie, LIU Gang, GUO Xiao-guang, WANG Guo-you. Effects of ShaoYangZhuGuFang on p16/Rb signaling pathway of naturally degenerative cynomolgus monkeys knee osteoarthritis model[J]. Chinese Journal of Clinical Anatomy. 2017, 35(6): 655-660 https://doi.org/10.13418/j.issn.1001-165x.2017.06.013

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