关键词:  , 哮喘模型；　雾化；　气管插管；　稳定性

Abstract: Objective　By comparing the modeling effect and stability of the mouse asthma model, to determine the best way to model.　Methods　BALB/c female mice of SPF grade for 6-8 weeks were randomly divided into 5 groups, including 20 μg sensitized atomizing inhalation challenge group (20-INH), 100-INH group, 20 μg sensitized intratracheal instillation challenge group (20-ITI) and 100-ITI group ,control group. Mice were sensitized and challenged with ovalbumin（OVA）to make asthmatic models . The mice were sensitized with 20 μg and 100 μg OVA on 0, 7 and 14 days respectively. From day 21, challenged by atomizing inhalation way or intratracheal instillation way. Within 24 h following the last challenge, the airway hyper responsiveness（AHR） of the mice was assessed. Bronchoalveolar lavage fluid (BALF) was taken for cells count and the left lung were examined pathologically. ELISA was used to detect the levels of serum total immunoglobulin E (IgE).　Results　The AHR, the total cells of BALF, the eosinophil count of BALF and serum total IgE level significantly increased in the groups of 20-INH, 100-INH and 100-ITI. All asthma groups had obvious airway inflammation infiltration and high mucus secretion. Among the groups, the 100-ITI was good at showing asthma characteristics. The standard deviation of each data of the model of intratracheal instillation was lower than that of the atomizing inhalation model.   Conclusions   The stimulation mode of intratracheal instillation can successfully establish a mouse asthma model at the sensitizing dose of 100 μg OVA, and the increasing of the airway mucus secretion and serum total IgE are more obvious. The model has high stability and is worthy of recommendation. 

Key words:  , Asthma model；　Atomizing inhalation；　Intratracheal instillation；　Stability