应用PeriCam PSI血流灌注散斑成像仪对食蟹猴模型舌象的初探研究

doi:10.13418/j.issn.1001-165x.2020.05.017

中国临床解剖学杂志 ›› 2020, Vol. 38 ›› Issue (5) : 578-582. DOI: 10.13418/j.issn.1001-165x.2020.05.017

李舒婷¹，　宗利丽¹，　何伟峰²，　饶军华³，　李比海³，　刘贻颜³，　刘玉洁¹
王庭伟⁴，　刘凌子¹，　伍庭凤⁵，　汪碧云²，　聂伟哲²

作者信息 +

The preliminary study of tongue pictures based on diseases-symptoms in cynomolgus monkey subjects by PeriCam PSI blood flow perfusion speckle imager

LI Shu-ting¹，ZONG Li-li¹，HE Wei-feng²，RAO Jun-hua³，LI Bi-hai³，LIU Yi-yan³，LIU Yu-jie¹，WANG Ting-wei⁴， LIU Ling-zi¹，WU Ting-feng⁵，WANG Bi-yun²，NIE Wei-zhe²

Author information +

文章历史 +

摘要

目的　探索运用PeriCam PSI血流灌注散斑成像仪检测食蟹猴模型舌部血液灌注量的方法并分析其可行性及应用价值。方法　（1）5只雌性食蟹猴行宫颈环扎术，制备食蟹猴子宫内膜异位症（endometriosis，EMs）疾病模型，1只雌性食蟹猴开腹仅观察盆腔情况。（2）分别于术前、手术当月及术后1、2、3个月运用PeriCam PSI血流灌注散斑成像仪测量所有食蟹猴舌面、舌底血液灌注量，采用与之配套的PIMSoft软件进行数据采集与分析。结果　（1）成功检测6只食蟹猴在不同时间段的舌部血液灌注量，宫颈环扎组食蟹猴术后的血液灌注量均呈现显著上升或下降，差异有统计学意义。（2）术后2月轻-中度盆腔粘连的食蟹猴舌面及舌底的血液灌注量均显著高于无粘连组，差异有统计学意义。结论　运用PeriCam PSI血流灌注散斑成像仪测量食蟹猴舌部血液灌注量的方法稳定可行，可能成为疾病病证结合模型舌象的量化评价方法。

Abstract

Objective　To explore how to use PeriCam PSI blood perfusion speckle imager to detect the blood perfusion in tongues of cynomolgus monkey subjects and analyze its feasibility and application values.　Methods　1. Five female cynomolgus monkeys were subjected to cervical cerclage in preparation for their endometriosis (EMs) models. One of them only served as an indicator of pelvic conditions after its laparotomy. 2. PeriCam PSI blood flow perfusion speckle imager was used to measure the blood perfusion on the surfaces and bases of tongues of all cynomolgus monkeys for their preoperative conditions, in the very month of the coming surgery, and s.p. 1th, 2nd and 3rd months respectively. PIMSoft, the matched software, was used in data collection and analysis.　Results　1. Blood perfusion volume on tongues of the 6 subjects at different phases was successfully captured and five out of them s.p. cervical cerclage were seen to undergo significant ups/downs of their blood perfusion volume with statistical significance in their discrepancy. 2. The blood perfusion volume of tongue surfaces and bases of cynomolgus monkeys with s.p. 2 months mild-moderate pelvic adhesion was significantly higher than that of non-adhesion counterparts, the difference being statistically significant.　Conclusions　The method of PeriCam PSI blood perfusion speckle imager to detect the blood perfusion in tongues of cynomolgus monkey subjects is stably feasible and might be incorporated in one of the quantitative evaluation methods for modeled tongue pictures based on diseases-symptoms.

导出引用

李舒婷, 宗利丽, 何伟峰, 饶军华, 李比海, 刘贻颜, 刘玉洁, 王庭伟, 刘凌子, 伍庭凤, 汪碧云, 聂伟哲. 应用PeriCam PSI血流灌注散斑成像仪对食蟹猴模型舌象的初探研究[J]. 中国临床解剖学杂志. 2020, 38(5): 578-582 https://doi.org/10.13418/j.issn.1001-165x.2020.05.017

LI Shu-ting, ZONG Li-li, HE Wei-feng, RAO Jun-hua, LI Bi-hai, LIU Yi-yan, LIU Yu-jie, WANG Ting-wei, LIU Ling-zi, WU Ting-feng, WANG Bi-yun, NIE Wei-zhe. The preliminary study of tongue pictures based on diseases-symptoms in cynomolgus monkey subjects by PeriCam PSI blood flow perfusion speckle imager [J]. Chinese Journal of Clinical Anatomy. 2020, 38(5): 578-582 https://doi.org/10.13418/j.issn.1001-165x.2020.05.017

中图分类号： R711.71

参考文献

[1] Humeau-Heurtier A, Mahé G, Abraham P. Microvascular blood flow monitoring with laser speckle contrast imaging using the generalized differences algorithm[J]. Microvascular Research, 2015,98:54-61.
[2] Eikermann-Haerter K, Lee J H, Yuzawa I, et al. Migraine mutations increase stroke vulnerability by facilitating ischemic depolarizations[J]. Circulation, 2012,125(2):335-345.
[3] Khan MB, Hoda MN, Vaibhav K, et al. Remote Ischemic Postconditioning: Harnessing Endogenous Protection in a Murine Model of Vascular Cognitive Impairment[J]. Translational Stroke Research, 2015,6(1):69-77.

[4] 宗利丽, 李亚里, 汪龙霞, 等. 子宫内膜异位症猕猴动物模型的建立[J]. 第一军医大学学报, 2003(10):1006-1009.

[5] Briers D, Duncan DD, Hirst E, et al. Laser speckle contrast imaging: theoretical and practical limitations[J]. Journal of biomedical optics, 2013, 18(6): 66018.

[6] Xie H, Zhang Y. Relationship between dynamic infrared thermal images and blood perfusion rate of the tongue in anaemia patients[J]. Infrared Physics & Technology, 2018, 89:27-34.
[7] 区绮云. 舌下络脉诊法诊查子宫内膜异位症的研究[D]. 广州中医药大学, 2008.
[8] Scheuzger JD, Zehnder A, Yeginsoy D, et al. Sublingual microcirculation：a case report[J]. Journal of Medical Case Reports, 2019,13(1).
[9] Imudia A, Kumar S, Saed G, et al. Pathogenesis of Intra-abdominal and Pelvic Adhesion Development[J]. Seminars in Reproductive Medicine, 2008, 26(4): 289-297.
[10]冯书梅. 慢盆逐瘀汤治疗慢性盆腔炎临床疗效及动物实验研究[D]. 广州中医药大学, 2011.
[11]冯晓冰. 剖宫产后盆腔粘连患者血清粘连相关指标与血流变指标的变化研究[J]. 中国医药科学, 2016, 6(22): 80-82.
[12]Zhang Y, Zhao L, Li J, et al. Microcirculation evaluation of facial nerve palsy using laser speckle contrast imaging: a prospective study[J]. European Archives of Oto-Rhino-Laryngology, 2019, 276(3): 685-692.
[13]Hoda MN, Fagan SC, Khan MB, et al. A 2 × 2 factorial design for the combination therapy of minocycline and remote ischemic perconditioning: efficacy in a preclinical trial in murine thromboembolic stroke model[J]. Experimental & Translational Stroke Medicine, 2014,6(1):10.