成年小鼠嗅觉功能与嗅上皮中成熟嗅觉神经元数量的实验研究
曾宪平, 陈合新, 付清玲, 徐睿, 文卫平, 史剑波, 许庚
中国临床解剖学杂志 ›› 2014, Vol. 32 ›› Issue (2) : 192-195.
成年小鼠嗅觉功能与嗅上皮中成熟嗅觉神经元数量的实验研究
Relationship between olfactory function and the number of mature olfactory neurons in olfactory epithelium of the adult mouse
目的 探讨成年BALB/C小鼠维持嗅觉功能所需嗅上皮中成熟嗅神经元的数量，研究小鼠嗅上皮中嗅神经元数量与小鼠嗅觉功能的相关性。 方法 用0.7%的Triton X-100灌注8~10周大小的BALB/C小鼠鼻腔以诱导小鼠嗅觉障碍，分别于灌鼻后第3，7，21，49，56天行觅食实验检测小鼠觅食行为的改变，并联合免疫荧光染色（IFC）的方法，检测小鼠嗅上皮（OE）中成熟嗅神经元（ORNs）的数量与其嗅觉功能的相关性。 结果 小鼠经Triton X-100灌注鼻腔后第3、7天，其觅食时间明显延长（F=32.04，P<0.001)，Bonferroni法两两比较示第3，7天觅食时间均长于对照组及造模后第21、49、56天组，P<0.001差异有统计学意义。嗅上皮中成熟嗅神经元（ORNs）的数量亦于第3、7天为低(F=223.97, P<0.001)，觅食时间与嗅上皮成熟ORNs的数量呈负相关（r=－0.757，P<0.001）。小鼠嗅觉障碍于造模后第21天左右开始恢复，此时嗅上皮中OMP（+）细胞占28.66%。 结论 小鼠嗅觉功能与其嗅上皮中成熟ORNs数量具有明显的相关性；当成年BALB/C小鼠嗅上皮中成熟ORNs数量恢复至对照组的28.66%时即可使小鼠嗅觉功能得以恢复。
Objective To explore the sufficient amount of mature olfactory receptor neurons(ORNs) in olfactory epithelium for maintaining normal olfactory function and to investigate the relationship between a simple olfactory test outcome and the mature olfactory neuronal population. Methods The anosmia model was induced in 8- to 10-week-old mice by intranasal irrigation with 0.7% Triton X-100 . The“buried food pellet” test was conducted at 3, 7, 21, 49, 56 days a after the anosmia model was established. The olfactory marker protein(OMP) olfactory epithelia(OE) was detected with immuno?uorescence to analyze the relationship“buried food pellet” test outcome and the mature olfactory neuronal population of OE. Results Olfactory marker protein(OMP) was physiologically expressed in mature olfacotry receptor neurons(ORNs) body, dendrites, and submucosa axons bundles. At 3rd,7th day after nose-irrigation with TritonX-100, time spend to find food was prolonged obviously(F =32.04，P <0.01)and was longer than that at 21th,49th,56th and control group when compared within each two groups with Bonferroni methode(P <0.01).The mature ORNs in OE was minimal at 3rd,7th after treatment (F =223.97, P <0.001). The food-?nding time was signi?cantly correlated with the number of mature ORNs in OE(r =－0.757, P <0.001).The anosmia mice begin to recover at 21th day after nasal irrigation, when the OMP(+) recovered to 28.66%. Conclusions Our studies confirmed that mice olfactory function is signi?cantly correlated with the number of mature ORNs in OE. The adult mice with anosmia could regain olfactory function when the mature ORNs in OE recovered to 28.66% of the control group.
嗅觉障碍 / 嗅觉标记蛋白 / OMP / 嗅觉 / tritonX-100
Dysosmia / Olfactory / OMP / TritonX-100
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