低温等离子消融术建立兔椎间盘退变模型的实验研究
周葳, 徐义春, 骆众星, 王其友, 余冬青, 韦家冬, 余灏涛, 赵飞
中国临床解剖学杂志 ›› 2013, Vol. 31 ›› Issue (6) : 696-701.
低温等离子消融术建立兔椎间盘退变模型的实验研究
Developing intervertebral disc degeneration at rabbit lumber spine by using low-temperature plasma radiofrequency ablation
目的 探讨低温等离子消融术建立兔椎间盘退变模型的方法与可行性。 方法 新西兰大白兔24只，随机分为实验组12只，对照组12只。两组均穿刺L3/4～L5/6椎间隙,实验组采用消融30s建模,对照组单纯以穿刺针穿刺建模。分别于术前及术后4、8、12周行CR、MRI检查及病理学检查。 结果 实验组DR及MR检查至术后12周均可见退变逐步加重的征象，如椎间隙高度的丢失及T2信号逐步降低。对照组DR及MR检查4周后无明显的退变加重迹象。两组病理学检查均未见早期的髓核缺失，实验组12周可见髓核正常结构消失，椎间盘内结构紊乱。 结论 低温等离子消融术比传统单纯穿刺更易及快速建立椎间盘退变的动物模型，采用此方法建立兔椎间盘退变模型是可靠可行的。
Objective To develop a new intervertebral disc(IVD) degeneration animal model by plasma radiofrequency ablation. Methods 24 New Zealand white rabbits were equally divided into the experimental group and control group. L3/4～L5/6 IVDs were punctured and ablated for 30 seconds in the experimental group and t were just punctured in the control group. DR imaging studies, MR imaging studies and histological analysis were performed prior to surgery and 4, 8, 12 weeks after surgery to monitor progression of IVDs degeneration. Results The DR and MR examinations showed degeneration signs like lost of IVD height and T2 density. The control group showed no significant changes in DR and MR scan after 4 weeks. Both groups showed no lost of nucleus pulposus in the early time points and the experimental group showed lost of nucleus pulposus and derangement of the IVD structure. Conclusions The practicable and reproducible animal IVD degeneration model proposed in this paper is more stable than the previous puncture model.
Low-temperature plasma radiofrequency ablation / Intervertebral disc degeneration / Animal model / Rabbit
[1] Singh K, Masuda K, An H. Animal models for human disc degeneration
[J].Spine J, 2005, 5（6）:267-279.
[2] Masuda K, Aota Y, Muehleman C, et al.A novel rabbit model of mild, reproducible disc degeneration by an anulus needle puncture: correlation between the degree of disc
injury and radiological and histological appearances of disc degeneration
[J]. Spine,2005,30(1):5-14.
[3] Pfirrmann C, Metzdorf A, Zanetti M, et al. Magnetic resonance classification of lumbar intervertebral disc degeneration
[J].Spine,2001,26(17):1873-1878.
[4] Cappello R, Bird J, Pfeiffer D,et al.Notochordal cell produce and assemble extracellular mMatrix in a distinct manner, which may be responsible for the maintenance of
healthy nucleus pulposus
[J].Spine,2006,31(8):873-882.
[5] Sobajima S, Kompel J, Kim J, et al. A slowly progressive and reproducible animal model of intervertebral disc degeneration characterized by MRI, X-Ray, and histology
[J].Spine,2005,30(1):15-24.
[6] Moon CH, Jacobs L, Kim JH,et al.Part 2: Quantitative proton T2 and sodium magnetic resonance imaging to assess intervertebral disc degeneration in a rabbit model
[J].Spine,2012,37(18):1113-1119.
[7] Zhang Y, Drapeau S, An HS,et al.Histological features of the degenerating interverterbral disc in a goat disc injury model
[J]Spine,2011,36(19):1519-27.
[8] Zhou RP, Zhang ZM, Wang L, et al.Establishing a disc degeneration model using computed tomography-guided percutaneous puncture techniquein the rabbit
[J].J Surg Res,2013
,181(2):65-74.
[9] Leckie SK, Bechara BP,Hartman RA, et al. Injection of AAV2-BMP2 and AAV2-TIMP1 into the nucleus pulposus slows the course of intervertebral discdegeneration in an in vivo
rabbit model
[J].Spine J,2012,12(1):7-20.
[10] Sowa G, Westrick E, Pacek C,et al. In vitro and in vivo testing of a novel regulatory system for gene therapy for intervertebral disc degeneration
[J]. Spine, 2011,36
(10):623-628.
[11] Hoogendoorn R, Helder M, Kroeze R,et al. Reproducible long-term disc degeneration in a large animal model
[J].Spine,2008,33(9):949-954.
[12]Zhang HN, Marca F, Hollister S, et al. Developing consistently reproducible intervertebral disc degeneration at rat caudal spine by using needle puncture
[J]. J Neurosurg
Spine,2009,10(6):522-530.
[13]Elliott DM，Yerramalli CS，Beckstein JC，et al. The effect of relative needle diameter in puncture and sham injection animal models of degeneration
[J]. Spine，2008，33（6）
：588-596.
[14]戎利民，张飞. 消融髓核成形术中消融时间与椎间盘内压变化关系的实验研究
[J].中国病理生理杂志，2008,24(10）：1975-1979.
[15]Gruber H,Johnson T,Norton J, et al. The sand rat model for disc degeneration: radiologic characterization of age-related changes
[J]. Spine，2002,27（3）：230-234.
[16]Hunter C, Matyas J, Duncan N. Cytomorphology of notochordal and chondrocytic cells from the nucleus pulposus: a species comparison
[J]. J Anat,2004,205(5):357-362.
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