大鼠脑出血后神经元线粒体的体视学分析及黄芪的神经保护作用研究
Analyses on stereology of mitochondrion of neurons that surround intracerebral hemorrhage in rats and the neuroprotective effect of astragalus injection for intracerebral hemorrhage
目的 探讨黄芪对大鼠脑出血后神经元线粒体的保护作用。 方法 用Ⅶ型胶原酶脑内注入法制作大鼠脑出血模型，利用Narrow-alley Test检测各组大鼠的神经行为学指标；用透射电镜观察各组大鼠脑出血灶周围神经元线粒体的超微结构并对其进行体视学分析；用免疫组织化学方法检测Caspase-3蛋白表达。 结果 神经行为学检测，模型组大鼠的不对称分值明显升高，治疗组大鼠不对称分值较模型组明显下降；神经元线粒体体视学分析，与模型组相比，治疗组线粒体的体密度、数密度、比表面积和比膜面积均较模型组增大，差异有显著性（P＜0.05)；与模型组比较，治疗组Caspase-3的阳性表达明显降低（P＜0.01)。 结论 黄芪能够减轻出血后神经元线粒体的损伤，抑制神经元凋亡，促进神经功能恢复。
Objective To study the protective effect of astragalus to the ultrastructure of apoptotic neurons which surround the intracerebral hemorrhage (ICH). Methods ICH model was established by injecting collagenase Ⅶ into the brain of rats. Method of Narrow-alley Test was applied to study behavioral index. The ultrastructure of neurons around the intracerebral hemorrhage in rats was observed by transmission electron microscope and a stereology analysis was performed on the mitochondria. Results mpared with model group, stereology analysis of nerve mitochondria, volume density, numerical density, specific surface and specific membrane of the astragalus treatment group increased notably(P＜0.05). Conclusion Astragalus could protect neurons located near the hemorrhage brain area and abate the neuronal apoptosis with this efficacy better at an early stage following hemorrhage.
Intracerebral hemorrhage / Astragalus injection / Stereology / Mitochondrion / Rat
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宁夏回族自治区自然科学基金（NZ0884）；自治区卫生厅重点科研计划（NW2011010）资助项目;宁夏师范学院科学研究基金资助
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