胸苷激酶1（TK1）在骨肉瘤中的表达及其临床病理意义
许美权, 王大平, 熊建义, 关弘, 吴俊辉, 黄雨华, 朱伟民, 李伟, 杨雅洁, 张继君
中国临床解剖学杂志 ›› 2013, Vol. 31 ›› Issue (5) : 564-567.
胸苷激酶1（TK1）在骨肉瘤中的表达及其临床病理意义
Expression of Thymidine kinase 1 (TK1) in osteosarcoma and its clinico-pathological significance
目的 探讨骨肉瘤肿瘤细胞胸苷激酶1（TK1）的表达状况及骨肉瘤患者血清TK1的水平，分析其临床病理意义。 方法 采用免疫组化MaxVision法检测40例骨肉瘤和20例骨巨细胞瘤组织标本中TK1的表达；点印迹免疫酶化学发光法检测20例骨肉瘤患者和20例骨外伤患者血清TK1的水平。 结果 骨肉瘤肿瘤细胞TK1阳性信号位于胞浆及胞核，高级别骨肉瘤阳性率显著高于低级别骨肉瘤（P<0.05）；骨肉瘤肿瘤细胞TK1阳性率亦显著高于骨巨细胞瘤（P<0.05）；骨肉瘤患者血清TK1的水平(2.95±1.33)pmol/L（0.97~7.25 pmol/L）高于外伤除外肿瘤患者(0.89±0.51) pmol/L (0.32~2.58 pmol/L)，两者差异具有显著性统计学意义（P<0.05）。 结论 TK1在骨肉瘤与骨的良性病变的鉴别诊断中具有一定的意义。此外，TK1在骨肉瘤患者血清中可被检测到较高的水平，是一项检测骨肉瘤增殖活性较灵敏的指标。
Objective To investigate the expression ofTK1 in osteosarcoma tumor cells and the TK1 level of TK1 in the serum of patients with osteosarcoma, and analyze their clinico-pathological significances. Methods 40 cases of osteosarcoma and 20 cases of giant cell tumor were included in this study. Immunohistochemical staining of TK1 was performed on the tumor tissue by MaxVision method. Serum TK1 level in 20 patients with osteosarcoma and 20 patients with bone trauma was tested by chemiluminescence dot blot enzyme chemistry. Results The reactivity assessed was in cytoplasm and nuclei. The positive rate of TK1 in high grade osteosarcoma was significantly higher than that in low grade osteasarcoma(P<0.05); The positive rate of TK1 in osteasarcomais was higher than that in giant cell tumor, and the difference was statistically significant(P<0.05). In addition, the serum TK1 content in patients with osteosarcoma is significantly higher than that in patients with bone traumatic [2.95 (0.97-7.25) pmol/Lvs 0.89 (0.32-2.58) pmol/L,P<0.05]. Conclusion TK1 has a certain value in the differential diagnosis of osteosarcoma from benign bone lesions. High level of TK1 can be tested in the serum in patient with osteosarcoma, and it seems that TK1 is a sensitive indicator for testing osteosarcoma proliferation.
Thymidine kinase 1 / Osteosarcoma / Immunohistochemical / Chemiluminescence
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