凋亡相关基因p53、bcl-2、bax在前列腺组织中的相关性

方志启, 吴刚, 王贺彬, 陈晓宇

中国临床解剖学杂志 ›› 2013, Vol. 31 ›› Issue (5) : 560-563.

中国临床解剖学杂志 ›› 2013, Vol. 31 ›› Issue (5) : 560-563.
实验研究

凋亡相关基因p53、bcl-2、bax在前列腺组织中的相关性

  • 方志启1, 吴刚1, 王贺彬1,  陈晓宇2
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Correlation of apoptosis genes of p53,bcl-2 and bax in tissue of prostate

  • FANG Zhi-qi1, WU Gang1, WANG He-bing1, CHEN Xiao-yu2
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摘要

目的 探讨细胞凋亡相关基因p53、bcl-2、bax在前列腺组织中的相关性。  方法 收集36例前列腺癌(prostate caner, PCa)、20例前列腺增生(benign prostatic hyperplasia,BPH)和11例正常前列腺(normal prostatic, NP),应用免疫组织化学S-P法检测凋亡相关基因p53、bcl-2、bax蛋白的表达。  结果 ①PCa和BPH组bcl-2蛋白阳性表达率明显高于NP组(P<0.05),而PCa组与BPH组阳性率差异无显著性。PCa组p53蛋白阳性表达率明显高于BPH组和NP组(P<0.01),而BPH组与NP组阳性率无显著性差异(P>0.05)。②p53与PCa分级有关,随着肿瘤分级增高而呈正相关(P<0.05);bcl-2与PCa分级有关,随着肿瘤分级增高而呈正相关(P<0.01),显示bcl-2、p53蛋白表达随着病理分级的增高而增高。PCa、BPH和NP中bax阳性表达率差异无显著性。③p53蛋白表达阳性率≤5年生存组明显高于>5年生存组,呈负相关(P<0.05);bcl-2、bax蛋白表达与生存期无关(P>0.05)。  结论    细胞凋亡相关基因 p53、bcl-2、bax蛋白的异常表达与PCa的发生和发展、病理分级和预后有相关性。  

Abstract

Objective To investigate clinical significance and expression between apoptosis and the expression of the related genes of p53,bcl-2 and bax in prostate cancer (PCa). Methods The expression of bcl-2, bax and p53 protein and apoptosis cells were detected by immunohistochemical S-P method respectively in tissues of 36 cases of  PCa, 20 BPH and 11 NP.   Results   ① It showed that bcl-2 expression in Pca was significantly higher than that in NP and BPH (P<0.05), and there was no significant difference between PCa and BPH(P>0.05). p53 expression in PCa was significantly higher than that in BPH and NP (P<0.01), and there was no significant difference between BPH and NP(P>0.05). ② In the tissue of Pca, p53 and bcl-2 expression was positively correlated with pathological grade, as PCa increased in grades (P<0.05, P<0.01).It showed that expression levels of bcl-2 and p53 protein in PCa tissues increased with the progression of pathological grade. Bax positive expressing rate difference had no significance in PCa, BPH and NP. ③The expression of p53 was frequently detected in shorter-than-5-year survival group, than it was in longer-than-5-year survival group(P<0.05). That of bcl-2 expression was not related to survival period(P>0.05).   Conclusion    The over-expression of apoptosis-related genes: bcl-2,bax and p53 might be related to the carcinogenesis,progression pathological grade and prognosis of prostate carcinoma

关键词

前列腺癌 / 基因 / bcl-2 / 免疫组织化学 / p53 / bax

Key words

Prostatic neoplasmas / Genes / bcl-2 / Immunohistochemistry / p53 / Bax

引用本文

导出引用
方志启, 吴刚, 王贺彬, 陈晓宇. 凋亡相关基因p53、bcl-2、bax在前列腺组织中的相关性[J]. 中国临床解剖学杂志. 2013, 31(5): 560-563
FANG Zhi-Qi, TUN Gang, WANG He-Ban, CHEN Xiao-Yu. Correlation of apoptosis genes of p53,bcl-2 and bax in tissue of prostate[J]. Chinese Journal of Clinical Anatomy. 2013, 31(5): 560-563
中图分类号:      R737.25    R697.3    R329.25   

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基金

安徽省自然科学基金资助项目(1208085MC53)


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