人参皂苷Rg1对脑缺血再灌注大鼠大脑细胞死亡方式的影响
Effects of ginsenoside Rg1 on cell death model in brain tissue after cerebral ischemia-reperfusion in rats
目的 探讨人参皂苷Rg1（ginsenoside Rg1）对脑缺血再灌注大脑皮层细胞死亡方式的影响。 方法 健康SD大鼠随机分为假手术组（Sham 组）、缺血再灌注组（Model组）、人参皂苷 Rg1 10、20、40 mg/kg处理组、尼莫地平处理组（阳性对照组），每组15只。各组于术前5 d至取材当日分别注射生理盐水（Sham、Model组）、人参皂苷Rg1（Rg1处理组）、尼莫地平（阳性对照组）。采用右侧大脑中动脉栓塞法制备脑缺血模型，动物清醒后进行神经功能评分和TTC染色验证模型是否成功；采用免疫组化法和免疫印迹法定性定量检测大脑皮层缺血再灌注24h后多聚二磷酸腺苷核糖聚合酶-1（poly ADP-ribose polymerase-1，PARP-1）、半胱氨酸蛋白酶-3（caspase-3）的表达。 结果 免疫组化和免疫印迹结果显示，Sham组大脑皮层区可见PARP-1、及少量caspase-3阳性细胞；Model组可见大量PARP-1阳性细胞及caspase-3阳性细胞；人参皂苷Rg1处理组神经功能缺损评分及PARP-1、caspase-3的表达显著低于 Model组。 结论 人参皂苷Rg1预处理对大鼠脑缺血再灌注具有保护作用，其机制可能与下调脑组织 PARP-1、caspase-3的表达，对抗脑细胞凋亡和胀亡有关。
Objective To study the mechanism of ginsenoside Rg1 after cerebral ischemia-reperfusion in adult rats. Methods Rats were randomly divided into the sham-operative group（sham group）, Model group, ginsenoside Rg1 10、20、40 mg/kg groups, Nimodipine group（drug control group）. Cerebral ischemia-reperfusion(CIR) model was made by occluding the middle cerebral artery in rats. After CIR, the neurological deficit score was evaluated and IHC and Western blot technique was used to observe the expressions of PARP-1 and caspase-3． Results Compared with Model group, the neurological deficit scores and the expressions of PARP-1 and caspase-3 were significantly decreased. Conclusion Ginsenoside Rg1 can protect the brain cells from damage after CIR injury by down-regulating the expression of PARP-1 and caspase-3.
人参皂苷Rg1 / 脑缺血再灌注 / 多聚二磷酸腺苷核糖聚合酶-1 / 半胱氨酸蛋白酶-3
Ginsenoside Rg1 / Cerebral ischemia-reperfusion / Poly ADP-ribose polymerase-1 / Caspase-3
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辽宁省自然科学基金项目（201102139）
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