人参皂苷Rg1对脑缺血再灌注大鼠大脑细胞死亡方式的影响

中国临床解剖学杂志 ›› 2013, Vol. 31 ›› Issue (5): 555-559.

人参皂苷Rg1对脑缺血再灌注大鼠大脑细胞死亡方式的影响

于利¹，　刘霞¹，　包翠芬2，　纪红超¹

1.辽宁医学院组胚教研室；　2.辽宁医学院科学实验中心, 辽宁锦州 121000

收稿日期:2012-10-11 出版日期:2013-09-25 发布日期:2013-10-16
通讯作者: 刘霞,教授,硕士生导师， E-mail: mianyizuhua@aliyun.com E-mail:mianyizuhua@aliyun.com
作者简介:于利（1983-），女，辽宁盘锦人，硕士，主要研究方向为脑缺血疾病基础研究
基金资助:
辽宁省自然科学基金项目（201102139）

Effects of ginsenoside Rg1 on cell death model in brain tissue after cerebral ischemia-reperfusion in rats

YU Li¹, LIU Xia¹, BAO Cui-fen², JI Hong-chao¹

1. Department of Histology and Embryology, 2.Centre of Scientific Expreiment, Liaoning Medical College, Jinzhou 121001,China

Received:2012-10-11 Online:2013-09-25 Published:2013-10-16

摘要/Abstract

摘要：

目的　探讨人参皂苷Rg1（ginsenoside Rg1）对脑缺血再灌注大脑皮层细胞死亡方式的影响。方法　健康SD大鼠随机分为假手术组（Sham 组）、缺血再灌注组（Model组）、人参皂苷 Rg1 10、20、40 mg/kg处理组、尼莫地平处理组（阳性对照组），每组15只。各组于术前5 d至取材当日分别注射生理盐水（Sham、Model组）、人参皂苷Rg1（Rg1处理组）、尼莫地平（阳性对照组）。采用右侧大脑中动脉栓塞法制备脑缺血模型，动物清醒后进行神经功能评分和TTC染色验证模型是否成功；采用免疫组化法和免疫印迹法定性定量检测大脑皮层缺血再灌注24h后多聚二磷酸腺苷核糖聚合酶-1（poly ADP-ribose 　polymerase-1，PARP-1）、半胱氨酸蛋白酶-3（caspase-3）的表达。结果　免疫组化和免疫印迹结果显示，Sham组大脑皮层区可见PARP-1、及少量caspase-3阳性细胞；Model组可见大量PARP-1阳性细胞及caspase-3阳性细胞；人参皂苷Rg1处理组神经功能缺损评分及PARP-1、caspase-3的表达显著低于　Model组。结论　人参皂苷Rg1预处理对大鼠脑缺血再灌注具有保护作用，其机制可能与下调脑组织　PARP-1、caspase-3的表达，对抗脑细胞凋亡和胀亡有关。　

关键词: 人参皂苷Rg1, 脑缺血再灌注, 多聚二磷酸腺苷核糖聚合酶-1, 半胱氨酸蛋白酶-3

Abstract:

Objective　To study the mechanism of ginsenoside Rg1 after cerebral ischemia-reperfusion in adult rats. Methods　Rats were randomly divided into the sham-operative group（sham group）, Model group, ginsenoside Rg1 10、20、40 mg/kg groups, Nimodipine group（drug control group）. Cerebral ischemia-reperfusion(CIR) model was made by occluding the middle cerebral artery in rats. After CIR, the neurological deficit score was evaluated and IHC and Western blot technique was used to observe the expressions of PARP-1 and caspase-3． Results Compared with Model group, the neurological deficit scores and the expressions of PARP-1 and caspase-3 were significantly decreased. Conclusion Ginsenoside Rg1 can protect the brain cells from damage after CIR injury by down-regulating the expression of PARP-1 and caspase-3.

Key words: Ginsenoside Rg1, Cerebral ischemia-reperfusion, Poly ADP-ribose polymerase-1, Caspase-3

中图分类号:

R364.12

于利, 刘霞, 包翠芬, 纪红超. 人参皂苷Rg1对脑缺血再灌注大鼠大脑细胞死亡方式的影响[J]. 中国临床解剖学杂志, 2013, 31(5): 555-559.

XU Li, LIU Xia, BAO Cui-Fen, JI Gong-Chao. Effects of ginsenoside Rg1 on cell death model in brain tissue after cerebral ischemia-reperfusion in rats[J]. Chinese Journal Of Clinical Anatomy, 2013, 31(5): 555-559.

参考文献

[1] Wyllie AH. Apoptosis: cell death in tissue regulation
[J].J Pathol,1987,153(4): 313-316.

[2] Chu X, Fu X, Zou L, et al. Oncosis, the possible cell death pathway in astrocytes after focal cerebral ischemia
[J]. Brain Res, 2007,29(1149):157-164.

[3] Shimizu S. Apoptosis and non-apoptotic cell death
[J].Rinsho Ketsueki,2011, 52(10):1669-1676.

[4] 李朝，邵姝元，郭敏，等.小鼠胚胎肾细胞凋亡与胀亡的超微结构
[J].解剖学杂志，2011，34（4）：484-486.

[5] Wang LB, Zhang LY, Shan CH.A new form of cell death: Parthanatos
[J].Yi Chuan,2010, 32(9):881-885.

[6] 辜清, 万勇, 吴娣, 等.大鼠脑缺血再灌注后运动皮质细胞凋亡的体视学观察
[J]. 南昌大学学报(理科版), 2009,33(1): 68-72.

[7] Sayk F, Bartels C.Oncosis rather than apoptosis
[J]? Ann Thorac Surg,2004,77(1): 382-383.

[8] Castejón OJ, Arismendi GJ. Nerve cell death types in the edematous human cerebral cortex
[J].J Submicrosc Cytol Pathol,2006,38(1):21-36.

[9] Koh DW, Dawson TM, Dawson VL. Mediation of cell death by poly(ADP-ribose) polymerase-1
[J]. Pharmacological research, 2005, 52(1):5-14.

[10] 贾红燕,李辉宇,赵浩亮.人参皂苷Rg1注射液对犬肝脏缺血再灌注损伤的保护作用
[J].山西医科大学学报，2010, 41(1)：8-10.

[11]Zhang X, Wang J,Xing Y,et al.Effects of ginsenoside Rg1 or 17β-estradiol on a cognitively impaired, ovariectomized rat model of Alzheimer's disease
[J]. Neuroscience,2012,18(220):191-200.

[12]Huang T,Fang F,Chen L,et al.Ginsenoside Rg1 attenuates oligomeric Aβ(1-42) induced mitochondrial dysfunction
[J].Curr Alzheimer Res,2012,9(3): 388-395.

[13] 李源莉.人参皂甙Rg1对脑缺血再灌注致小鼠皮质神经元损伤的保护作用及其机制探讨
[D].西安:第四军医大学,2007.

[14]包翠芬.人参皂苷Rg1对抗脑缺血再灌注细胞凋亡及p-JNK表达的实验研究
[D].锦州:辽宁中医药大学,2009.

[15]刘霞.人参皂苷Rg1对抗脑缺血再灌注ERK通路的实验研究
[D].锦州:辽宁中医药大学,2010.