TH和DβH在海洛因戒断、复吸大鼠脑VTA、NAc区神经元的表达
李一欣, 梁文妹, 洪艳, 夏白娟, 张璞
中国临床解剖学杂志 ›› 2013, Vol. 31 ›› Issue (5) : 547-550.
TH和DβH在海洛因戒断、复吸大鼠脑VTA、NAc区神经元的表达
Effects of heroin withdrawal and relapse on the expression of TH and DβH in the neurons of VTA and NAc regions of rat brain
目的 观察海洛因戒断、复吸对大鼠脑VTA、NAc神经元表达酪氨酸羟化酶（TH）和多巴胺β羟化酶（DβH）的影响，探讨多巴胺（DA）和去甲肾上腺素（NE）在海洛因戒断、复吸过程中的调节机制及相互关系。 方法 将正常雄性SD大鼠63只，随机分为实验组21只、盐水对照组21只和正常对照组 21只，实验组大鼠又分海洛因戒断组、美沙酮脱毒组和海洛因复吸组。取正常对照组、盐水对照组及实验各组大鼠的脑组织，用免疫组化SABC法显示VTA、NAc区TH、DβH阳性神经元并用图像分析法测定平均光密度值。 结果 与正常及盐水对照组比较：（1）海洛因戒断组和复吸组大鼠VTA、NAc内TH表达均增强，经美沙酮脱毒之后恢复正常；（2）DβH的表达在戒断组大鼠VTA、NAc内有所增强，但在脱毒组和复吸组均减弱。 结论 在海洛因戒断、脱毒和复吸期间，VTA、NAc区神经元的DA和NE相互调节，是产生奖赏效应的重要神经递质。
Objective To investigate the effect of the heroin withdrawal and relapse on the expression of TH and DβH in the ventral tegmental area(VTA) and nucleus accumbens(NAc) regions, and explore the adjustment mechanism and the relationship between dopamine(DA) and norepinephrine(NE). Methods 63 adult, male SD rats were randomly divided into three groups: normal control group (NCG), saline control group (SCG) and experiment group (EG). Experiment group was subdivided into: heroin withdrawal group (HWG), methadone detoxication group (MDG) and heroin relapse group (HRG). Brain tissues were excised after models were set up. Immunohistochemical staining was performed to test the expression of TH and DβH in the VTA and NAc regions. Results (1) TH-immunoreactive neurons expressed more TH in the VTA and NAc regions from HWG and HDG rats than that from SCG and NCG rats. After methadone detoxication, the expression of TH became normal. (2) DβH-immunoreactive neurons expressed more DβH in HWG and less DβH in MDG and HRG compared with SCG and NCG. Conclusions There is interaction between TH and DβH in the neurons of VTA and NAc regions during heroin withdrawal, methadone detoxication and heroin relapse period, and these two substances are important neurotransmitters which produce rewarding effect.
/ 海洛因 / 中脑腹侧被盖区 / 伏隔核 / 酪氨酸羟化酶 / 多巴胺&beta / 羟化酶
Heroin / Ventral tegmental area / Nucleus accumbens / TH / DβH
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国家自然科学基金（81260207）；贵州省科技攻关项目（黔科合SY字[2012]3141号）；贵州省优秀科技教育人才省长专项资金项目（黔省专合字[2011]48号）
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