目的　为眉间区除皱术提供表情肌解剖学形态学基础。  方法　对6例(12侧)成人头部标本的降眉肌、皱眉肌、降眉间肌进行大体解剖，测量相关表情肌构筑数，照像留存。通过人工干预后进行CT扫描，简便快捷的建立三维模型，清晰的定位表情肌。  结果　通过解剖，清晰地展示了眉间区域表情肌的形态学特点，并测量出其相关定位参数及肌重，成功地建立了眉间区域表情肌的三维可视化模型。眉间区域表情肌包括皱眉肌和眉间降肌，皱眉肌形态可分为两大类：横向型和斜向型，一般从眉毛形态即可辨识。其中，斜型按其形态又可分为：斜向单束型和斜向分束型。眉间降肌可分为降眉肌、降眉间肌，其形态及发育情况个体差异较大。  结论　眉间区表情肌为肉毒素注射治疗眉间纹的目标肌肉，通过探讨眉间区表情肌解剖位置及形态特征, 测量相关表情肌构筑数并简便快捷的建立三维模型，对个性化肉毒素除皱开展有临床指导意义。

Objective　To provide anatomical and morphologic basis for facial rejuvenation.  Method　6 cases (12 sides) adult cephalic specimens were anatomized.  The landmarks of the glabella muscles, including the corrugator, the depressor supercilii and the procerus were measured. The weights of the muscle were also examined and photographed. CT scan data of heads were obtained after human intervention, and 3D model was set up, demonstrating glabella muscles clearly.　Results　The clinical anatomy clearly showed the anatomical basis of glabella muscles,  the glabella muscles included corrugator and glabella depressor.  Corrugator could be divided into two broad categories: transverse and oblique, which could be generally recognized from the morphology of the eyebrows. Among them, the oblique type could be divided into oblique single beam type and oblique beam type according to its shape. the glabella depressor included depressor supercilii and Procerus. There was individualized morphology and development of the glabella depressor.　Conclusions　 The glabella muscles are the potent targets for the use of botox injection. Through exploration of the anatomical and morphologic characteristics of the glabella muscles, measurement of related data, 3d model can be easily set up, which can demonstrate the glabella clearly. It is of great clinical significance for customizing the use of Botulinum Toxin Injection.