维生素C对MPP+致帕金森病细胞模型中PC12细胞凋亡的影响
Effects of vitamin C on PC12 cells apoptosis in MPP+ induced cell model of Parkinson's Disease
目的 探讨维生素C（Vit C）对MPP+致帕金森病细胞模型中PC12细胞凋亡的影响及作用机制。 方法 培养神经细胞株PC12细胞，将其分为单纯帕金森病组（MPP+组）和Vit C治疗组（Vit C组），Vit C组加入250 μmol/L Vit C孵育6 h，之后向两组均加入100 μmol/L MPP+，在体外模拟帕金森病的神经损伤，孵育12 h后，利用乳酸脱氢酶（LDH）试剂盒检测LDH变化；TUNEL法检测鼠脑神经元凋亡情况，并利用Western-blot检测神经元中c-Caspase3和Caspase表达情况。 结果 与MPP+组比较，Vit C能明显减轻帕金森病PC12细胞凋亡，显著改善细胞生存环境； TUNEL检测结果显示，Vit C能明显减少PC12细胞凋亡。同时，Western-blot结果提示Vit C下调c-Caspase3蛋白的表达，组间差异有统计学意义( P<0.05)。 结论 Vit C可以直接抑制c-Caspase3表达，减少细胞凋亡，可能对帕金森病造成的细胞凋亡具有一定的拮抗作用。
Objective To investigate the effect and the related mechanism of Vitamin C (Vit C) on PC12 cells apoptosis in MPP+ induced cell model of Parkinson's disease (PD). Methods PC12 cells were divided to MPP+ induced cell model of PD group (MPP+ group) and Vit C treatment group (Vit C group). Firstly, Vit C group was incubated with 250 μmol/L Vit C 6 h, and then the two groups were treated with 100 μmol/L MPP+ 12 h. The LDH concentration was measured by LDH kit. The TUNEL method was performed to measure PC12 cells apoptosis in two groups. The expression of Caspase3 and c-Caspase3 was detected by Western-blot. Results Compared with MPP+ group, Vit C significantly inhibited LDH concentration and decreased apoptosis of PC12 cells, indicating that Vit C could down-regulate the expression of c-Caspase3 (P<0.05). Conclusion Vit C can decrease apoptosis of PC12 cells in vitro significantlyand play important protection role in PD via down-regulation of the expression of c-Caspase3
帕金森病 / TUNEL法 / 凋亡 / 维生素C / MPP+
Parkinson's disease / TUNEL method / Apoptosis / Vitamin C / MPP+
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陕西省教育厅基金（2013JK0755）；陕西省卫生厅基金（2010D57）；西安医学院博士科研启动基金（2012DOC11）；西安医学院校级基金（2012FZ14）
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