维生素C对MPP+致帕金森病细胞模型中PC12细胞凋亡的影响

中国临床解剖学杂志 ›› 2013, Vol. 31 ›› Issue (4): 453-456.

维生素C对MPP+致帕金森病细胞模型中PC12细胞凋亡的影响

曹健¹，　王瑞²，　王倩¹，　王爽³

1. 西安医学院生理学教研室，西安 710021；　2. 西安交通大学医学院第一附属医院中医科，西安 710061；
3. 西安医学院病理生理学教研室，西安 710021

收稿日期:2013-04-26 出版日期:2013-07-25 发布日期:2013-07-24
通讯作者: 王倩，讲师，医学博士，Tel:029-86177559，E-mail: wq816　@126.com E-mail:littlegrass123@163.com
作者简介:曹健（1975- ），男，讲师，医学硕士，主要从事帕金森病发病的研究，Tel:029-86177559
基金资助:
陕西省教育厅基金（2013JK0755）；陕西省卫生厅基金（2010D57）；西安医学院博士科研启动基金（2012DOC11）；西安医学院校级基金（2012FZ14）

Effects of vitamin C on PC12 cells apoptosis in MPP+ induced cell model of Parkinson's Disease

CAO Jian¹，WANG Rui² ，WANG Qian¹, WANG Shuang³

1.Department of Physiology, Xi'an Medial College, Xi'an 710021, China； 2.Traditional Chinese Medicine, First Affiliated Hospital of medical college of Xi'an Jiaotong University, xi'an, 710061,China; 3.Department of Pathophysiology, Xi'an Medial College, Xi'an 710021, China

Received:2013-04-26 Online:2013-07-25 Published:2013-07-24

摘要/Abstract

摘要：

目的　探讨维生素C（Vit C）对MPP+致帕金森病细胞模型中PC12细胞凋亡的影响及作用机制。方法　培养神经细胞株PC12细胞，将其分为单纯帕金森病组（MPP+组）和Vit C治疗组（Vit C组），Vit C组加入250 μmol/L Vit C孵育6 h，之后向两组均加入100 μmol/L MPP+，在体外模拟帕金森病的神经损伤，孵育12 h后，利用乳酸脱氢酶（LDH）试剂盒检测LDH变化；TUNEL法检测鼠脑神经元凋亡情况，并利用Western-blot检测神经元中c-Caspase3和Caspase表达情况。结果　与MPP+组比较，Vit 　C能明显减轻帕金森病PC12细胞凋亡，显著改善细胞生存环境； TUNEL检测结果显示，Vit C能明显减少PC12细胞凋亡。同时，Western-blot结果提示Vit C下调c-Caspase3蛋白的表达，组间差异有统计学意义( P<0.05)。结论　Vit C可以直接抑制c-Caspase3表达，减少细胞凋亡，可能对帕金森病造成的细胞凋亡具有一定的拮抗作用。

关键词: 帕金森病, TUNEL法, 凋亡, 维生素C, MPP+

Abstract:

Objective　To investigate the effect and the related mechanism of Vitamin C (Vit C) on PC12 cells apoptosis in MPP+ induced cell model of Parkinson's disease (PD).　Methods　PC12 cells were divided to MPP+ induced cell model of PD group (MPP+ group) and Vit C treatment group (Vit C group). Firstly, Vit C group was incubated with 250 μmol/L Vit C 6 h, and then the two groups were treated with 100 μmol/L MPP+ 12 h. The LDH concentration was measured by LDH kit. The TUNEL method was performed to measure PC12 cells apoptosis in two groups. The expression of Caspase3 and c-Caspase3 was detected by Western-blot. Results Compared with MPP+ group, Vit C significantly inhibited LDH concentration and decreased apoptosis of PC12 cells, indicating that Vit C could down-regulate the expression of c-Caspase3 (P<0.05).　Conclusion　Vit C can decrease apoptosis of PC12 cells in vitro significantlyand play important protection role in PD via down-regulation of the expression of c-Caspase3

Key words: Parkinson's disease, TUNEL method, Apoptosis, Vitamin C, MPP+

中图分类号:

R651.15
R962

曹健, 王瑞, 王倩, 王爽. 维生素C对MPP+致帕金森病细胞模型中PC12细胞凋亡的影响[J]. 中国临床解剖学杂志, 2013, 31(4): 453-456.

CAO Jian, WANG Rui, WANG Qian, WANG Shuang. Effects of vitamin C on PC12 cells apoptosis in MPP+ induced cell model of Parkinson's Disease[J]. Chinese Journal Of Clinical Anatomy, 2013, 31(4): 453-456.

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