不同剂量可卡因对昆明小鼠成瘾行为学的影响

中国临床解剖学杂志 ›› 2013, Vol. 31 ›› Issue (3): 325-327.

不同剂量可卡因对昆明小鼠成瘾行为学的影响

陈振中¹, 　顾晶晶¹，　李娟¹，　张磊²，　崔梦卿³，　李良平⁴，　张璐¹

1.南方医科大学病理生理学教研室；广东省功能蛋白质组学重点实验室；　2.南方医科大学组织学与胚胎学教研室，广州510515；
3.南方医科大学南方医院门诊部，广州 510515；　4.广州市红十字会医院神经外科，广州 510220

收稿日期:2013-04-01 出版日期:2013-05-25 发布日期:2013-06-06
通讯作者: 张璐，教授，博士生导师，Tel：(020)61648726，E-mail: zlulu70@126.com E-mail:chenzhenzhong1989@126.com
作者简介:陈振中（1989-），男，江西赣州人，在读硕士，主要从事可卡因成瘾机制的研究，Tel：020-62789374
基金资助:
国家自然科学基金(81071120)；教育部新世纪优秀人才支持计划（NCET-09-0088）；教育部科学技术研究重点项目(21132)；粤港关键领域重点突破项目(2011A011304001)；广东省产学研合作重大科技专项(2011A090100025)；广州市科技计划项目(7411832133935)；广东省自然科学基金（10151022001000003）

Various effect of dose-dependent cocaine on the addicted behavior of KM mice

CHEN Zhen-zhong¹, GU Jing-jing¹, LI Juan¹, ZHANG Lei², CUI Meng-qing³，LI Liang-ping⁴, ZHANG Lu¹

1.Department of Pathphysiology and Key Laboratory of Proteomics of Guangdong Province, Southern Medical University, Guangzhou 510515, China; 2.Department of Histology and embryology, Southern Medical University, Guangzhou 510515, China; 3.Outpatient Department, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China; 4.Neurosurgery department of Guangzhou Red Cross Hospital, Guangzhou 510220

Received:2013-04-01 Online:2013-05-25 Published:2013-06-06

摘要/Abstract

摘要：

目的　研究昆明小鼠对不同剂量可卡因的敏感度，为昆明小鼠在神经科学领域的应用，特别是可卡因成瘾的研究提供参考。方法同龄（8~12周）的雄性昆明小鼠，分别腹腔注射10 mg/kg、20 mg/kg 的可卡因，条件位置偏爱实验（Conditioned place preference）和行为敏化实验(Behavior sensitization)检测小鼠行为学变化,观察昆明小鼠在不同剂量可卡因刺激下的行为学变化，以此来确定昆明小鼠对不同剂量可卡因的敏感度。结果　在条件位置偏爱实验中，可卡因 20 mg/kg剂量组(N=11)与可卡因10 　mg/kg剂量组(N=12)相比，第9天与第2天在白箱中的时间差score(post-pre)有统计学差异(P<0.05)；而在行为敏化实验中，可卡因20 mg/kg组(N=8)与可卡因10mg/kg组(N=12)相比，自主活动次数明显增加，有统计学差异(P<0.05)；在第16 天给予一针与之前同剂量的可卡因，可卡因20 mg/kg组与可卡因10mg/kg组相比，自主活动次数有统计学差异(P<0.05)。结论　昆明小鼠在20 mg/kg可卡因的刺激下，能产生更为明显的条件位置偏爱以及更加强烈的行为敏化反应，是较为理想的可卡因成瘾小鼠模型。

关键词: , 昆明小鼠, 剂量, 条件位置偏爱, 行为敏化

Abstract:

Objective　To investigate the sensitivity of Kunming mice to different doses of cocaine, and provide reference for the cocaine related research of Kunming mice.　Methods　Kunming mice, male, aged 8~12 weeks, were used. They were randomly divided into two groups. One group was injected with 10 mg/kg cocaine, while the other 20 mg/kg cocaine. Conditioned place preference and Behavior sensitization were used to measure the behavioral differences before and after cocaine treatment.　Results　In the conditioned place preference analysis, the time difference in the white box on day 9 and day 2 (post-pre) was statistically significant（P<0.05） between the two groups. In the Behavior sensitization test, the ambulatory accounts between the two groups were found to be statistically significant（P<0.001）during the time treated with cocaine. And then they were put back to the colony room for 1 week before they received a challenging dose of cocaine. On the challenging day, the ambulatory accounts were found to be statistically significant between them (P=0.001).　Conclusion　Kunming mice can produce more obvious conditioned place preference and more intense behavioral sensitization reaction when treated with 20 mg/kg cocaine.

Key words: KM mice, Dose, Conditioned place preference, Behavioral sensitization

中图分类号:

R338

陈振中, 顾晶晶, 李娟, 张磊, 崔梦卿, 李良平, 张璐. 不同剂量可卡因对昆明小鼠成瘾行为学的影响[J]. 中国临床解剖学杂志, 2013, 31(3): 325-327.

CHEN Zhen-Zhong, GU Jing-Jing, LI Juan, ZHANG Lei, CUI Meng-Qing, LI Liang-Beng, ZHANG Lu. Various effect of dose-dependent cocaine on the addicted behavior of KM mice[J]. Chinese Journal Of Clinical Anatomy, 2013, 31(3): 325-327.

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