关键词:  , RSC96细胞, 巨噬细胞, NGF, Laminin, 共培养

Abstract:

Objective　To study the expression of NGF and Laminin in RSC96 cells under co-culture with macrophages in different activation states.　Methods　A Transwell system was built to co-culture RSC96 cells with rat peritoneal macrophages. Real-time PCR, western blotting, and ELISA were used to detect NGF and LN expression in each group.　Results　Under macrophage co-culture, the NGF expression in RSC96 cells was increased. A significant promotion has been found with activated macrophages. With regard to LN expression, no significant difference was detected. 　Conclusions　The macrophage could contribute to the NGF expression in RSC96 cells, the effect of which is influenced by the activation states. Activated macrophages showed greater effect. No such promotion is found on LN expression, which is associated more with the migration of the Schwann cells. Further research is needed.

Key words: RSC96 cells, Macrophages, NGF, Laminin, co-cultured