LIM矿化蛋白1过表达对骨肉瘤细胞系OS-9901细胞增殖的影响
严广斌, 余楠生, 卢伟杰, 卢永辉, 钱东阳
中国临床解剖学杂志 ›› 2013, Vol. 31 ›› Issue (3) : 314-317.
LIM矿化蛋白1过表达对骨肉瘤细胞系OS-9901细胞增殖的影响
The effects of LIM mineralization protein 1 over-expression on the osteosarcoma cell line OS-9901 proliferation
目的 探讨骨肉瘤细胞系OS-9901细胞中LIM矿化蛋白1（LMP-1）过表达对该细胞增殖的影响。 方法 构建LMP-1过表达慢病毒载体，通过脂质体转染进骨肉瘤细胞系OS-9901细胞。通过荧光定量PCR和Western blot检测LMP-1过表达情况，并通过BrdU掺入实验比较LMP-1过表达前后细胞增殖情况。 结果 转染了LMP-1过表达慢病毒载体6 h后细胞内LMP-1 mRNA和蛋白表达均无显著变化（P>0.05）,且DNA合成量无显著变化。而当LMP-1过表达慢病毒载体转染细胞12、24和48 h后细胞内LMP-1 mRNA和蛋白表达量均显著提高的同时，DNA合成量显著降低（P<0.05）。 结论 LMP-1过表达可抑制骨肉瘤细胞系OS-9901细胞增殖。
Objective To investigate the effects of LIM mineralization protein 1 over-expression on the osteosarcoma cell line OS-9901 proliferation. Methods LIM mineralization protein overexpression lentiviral vector was constructed and transfected into osteosarcoma cell line OS-9901 cells by liposome. Quantitative PCR and Western blot were performed to measure the relative mRNA and protein expression of LIM mineralization protein 1, and BrdU assay was performed to detect cell proliferation before and after the LMP-1 over-expression. Results LMP-1 mRNA and protein expression did not change significantly (P>0.05) at 6 h after cells transfected with the LMP-1 expression lentiviral vector. When LMP-1 mRNA and protein expression were significantly increased at 12h, 24h and 48h after cells transfected with the LMP-1 expression lentiviral vector, DNA synthesis was significantly lower (P<0.05). Conclusion LIM mineralization protein 1 overexpression can inhibit osteosarcoma cell line OS-9901 cell proliferation.
LIM mineralization protein 1 / Osteosarcoma cells / Lentiviral vector
[1] Boden SD, Liu Y, Hair GA, et al. LMP-1, a LIM-domain protein, mediates BMP-6 effects on bone formation
[J]. Endocrinology,1998,139(12): 5125-5134.
[2] Strohbach C, Kleinman S, Linkhart T, et al.Potential involvement of the interaction between insulin-like growth factor binding protein (IGFBP)-6 and LIM mineralization protein (LMP)-1 in regulating osteoblast differentiation
[J]. J Cell Biochem,2008,104(5):1890-1905.
[3] Xia H, Diebold D, Nho R, et al. Pathological integrin signaling enhances proliferation of primary lung fibroblasts from patients with idiopathic pulmonary fibrosis
[J]. J Exp Med,2008,205(7): 1659-1672.
[4] Meijerink J, Mandigers C, de Locht LV, et al. A novel method to compensate for different amplification efficiencies between patient DNA samples in quantitative real-time PCR
[J]. J Mol Diagn, 2001, 3(2): 55-61.
[5] 刘会文, 罗嘉全, 韩智敏, 等. LIM矿化蛋白1促进成骨的研究现状
[J]. 中国骨与关节外科，2011，4(4): 334-337.
[6] 刘辉, Bargouti M, 郑召民，等. LIM矿化蛋白-1抑制破骨前体细胞合成一氧化氮的分子机制研究
[J]. 中华骨科杂志，2011，31(5): 520-529.
[7] 蒲超, 倪卫东, 高仕长, 等. 人LIM矿化蛋白1基因腺病毒重组表达载体构建及在犬骨髓间充质干细胞中的表达
[J]. 中国组织工程研究与临床康复，2011，15(19): 3433-3437.
[8] 元永学, 王亚军, 赵凡. CD44基因在骨肉瘤中的表达及意义
[J]. 中国实验诊断学，2012，16(4): 756-757.
[9]吴晓春, 杨春雷, 王建军, et al. EZH2mRNA在骨肉瘤中的表达及意义
[J]. 广东医学,2012,33(18): 2815-2816.
[10]邓文斌. Ezrin介导骨肉瘤转移机制的相关研究
[J]. 重庆医学,2012,41(11): 1085-1088.
[11]蒋朋翔, 刘光耀, 梁柏坚, 等. MMP-9和Snail在骨肉瘤中的表达及其诊断意义
[J]. 中国实验诊断学，2012，16(5): 916-917.
[12]刘宣毅, 贾卫斗, 侯瑞, 等. Snail及N-cadherin蛋白在骨肉瘤中的表达及其与预后的关系
[J]. 肿瘤防治研究,2012,39(5): 551-554.
[13]多健, 王国文, 韩秀鑫, 等. APE1基因沉默增强骨肉瘤 U2-OS 细胞硼替佐米治疗敏感性的实验研究
[J]. 中国肿瘤临床，2012，39(8): 429-432.
[14]范磊, 邵增务, 吴强, 等. MicroRNA-145对骨肉瘤细胞增殖及凋亡的影响
[J]. 中国癌症杂志，2012，22(4): 247-251.
京ICP备08002354号-3/
| 〈 |
|
〉 |
