LIM矿化蛋白1过表达对骨肉瘤细胞系OS-9901细胞增殖的影响

中国临床解剖学杂志 ›› 2013, Vol. 31 ›› Issue (3): 314-317.

LIM矿化蛋白1过表达对骨肉瘤细胞系OS-9901细胞增殖的影响

严广斌,　余楠生,　卢伟杰, 　卢永辉,　钱东阳

广州医学院第一附属医院骨科，广州 510120

收稿日期:2013-03-10 出版日期:2013-05-25 发布日期:2013-06-06
通讯作者: 余楠生,主任医师,硕士生导师,E-mail：cjojs@126.com E-mail:13543430306@139.com
作者简介:严广斌(1972-)，男，在读博士，副主任医师，主要研究领域为关节外科

The effects of LIM mineralization protein 1 over-expression on the osteosarcoma cell line OS-9901 proliferation

YAN Guang-bin， Yu Nan-sheng, LU Wei-jie,LU Yong-hui, QIAN Dong-yang

Department of Orthopaedics，the First Affiliated Hospital of Guangzhou Medical College，Guangzhou 510120,China

Received:2013-03-10 Online:2013-05-25 Published:2013-06-06

摘要/Abstract

摘要：

目的　探讨骨肉瘤细胞系OS-9901细胞中LIM矿化蛋白1（LMP-1）过表达对该细胞增殖的影响。方法　构建LMP-1过表达慢病毒载体，通过脂质体转染进骨肉瘤细胞系OS-9901细胞。通过荧光定量PCR和Western blot检测LMP-1过表达情况，并通过BrdU掺入实验比较LMP-1过表达前后细胞增殖情况。结果　转染了LMP-1过表达慢病毒载体6 h后细胞内LMP-1 mRNA和蛋白表达均无显著变化（P>0.05）,且DNA合成量无显著变化。而当LMP-1过表达慢病毒载体转染细胞12、24和48 h后细胞内LMP-1 mRNA和蛋白表达量均显著提高的同时，DNA合成量显著降低（P<0.05）。结论　LMP-1过表达可抑制骨肉瘤细胞系OS-9901细胞增殖。

关键词: LIM矿化蛋白1, 骨肉瘤细胞, 慢病毒载体

Abstract:

Objective　To investigate the effects of LIM mineralization protein 1 over-expression on the osteosarcoma cell line OS-9901 proliferation.　Methods　LIM mineralization protein overexpression lentiviral vector was constructed and transfected into osteosarcoma cell line OS-9901 cells by liposome. Quantitative PCR and Western blot were performed to measure the relative mRNA and protein expression of LIM mineralization protein 1, and BrdU assay was performed to detect cell proliferation before and after the LMP-1 over-expression.　Results　LMP-1 mRNA and protein expression did not change significantly (P>0.05) at 6 h after cells transfected with the LMP-1 expression lentiviral vector. When LMP-1 mRNA and protein expression were significantly increased at 12h, 24h and 48h after cells transfected with the LMP-1 expression lentiviral vector, DNA synthesis was significantly lower (P<0.05).　Conclusion　LIM mineralization protein 1 overexpression can inhibit osteosarcoma cell line OS-9901 cell proliferation.

Key words: LIM mineralization protein 1, Osteosarcoma cells, Lentiviral vector

中图分类号:

Q813.11

严广斌, 余楠生, 卢伟杰, 卢永辉, 钱东阳. LIM矿化蛋白1过表达对骨肉瘤细胞系OS-9901细胞增殖的影响[J]. 中国临床解剖学杂志, 2013, 31(3): 314-317.

YAN An-Bin, TU Nan-Sheng, LEI Wei-Jie, LEI Yong-Hui, JIAN Dong-Yang. The effects of LIM mineralization protein 1 over-expression on the osteosarcoma cell line OS-9901 proliferation[J]. Chinese Journal Of Clinical Anatomy, 2013, 31(3): 314-317.

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