维甲酸对成纤维细胞及脂肪源干细胞成骨诱导的影响
刘玉峰, 戴景兴, 杨春, 周辛, 曲戎梅, 李鹏, 区颖华, 原林
中国临床解剖学杂志 ›› 2013, Vol. 31 ›› Issue (3) : 303-307.
维甲酸对成纤维细胞及脂肪源干细胞成骨诱导的影响
Effects of retinoic acid in the osteogenic induction to dermal fibroblasts and adipose derived stem cells
目的 探究维甲酸（RA）对乳鼠真皮成纤维细胞（DFB）及脂肪源干细胞（ADSCs）成骨诱导分化的影响。 方法 分别离体培养5~7 d龄乳鼠DFB及ADSCs。对DFB进行波形蛋白的免疫荧光鉴定，对ADSCs进行成骨、成脂肪诱导并做表面标记物鉴定。用RA及传统成骨诱导液分别对两类细胞进行成骨诱导2周，茜素红染色检测细胞内钙结节的形成，酶标仪检测细胞诱导后ALP 的OD值。 结果 两类细胞在常规成骨诱导及RA诱导成骨后，均有钙结节形成。传统成骨诱导与RA成骨诱导组与各自对照组相比，ALP活性均升高。在DFB组中，RA诱导的ALP活性比传统成骨诱导高，而在ADSCs组中，结果相反。 结论 RA可以促进DFB及ADSCs的成骨分化，并且因细胞种类不同而显著效果不同。
Objective To explore the effect of retinoic acid(RA) on ALP activity during the osteogenic differentiation of dermal fibroblasts(DFB) and adipose derived stem cells(ADSCs).Method DFB and ADSCs were isolated from suckling mouse aged 5~7 days and cultured in vitro. The DFB were identified by immunofluorescence of vimentin, and the ADSCs were identified by osteogenic differentiation, adipogenic differentiation and cell surface markers detection. Induced by RA and traditional osteogenic liquid separately for 2 weeks, ALP activities were measured by OD value and all specimens were stained by alizarin red. Results All the specimens were stained by alizarin red, except the control groups. Compared with the control groups, all the induced groups displayed a higher activity of ALP. It is worth noting that ALP expression is higher induced by RA than induced by traditional osteogenic liquid. But the opposite results were obtained in the ADSCs groups. Conclusion RA is also responsible for osteogenic differentiation of DFB and ADSCs; what is more, the results may be different due to the varieties of cells.
筋膜学 / 维甲酸 / 成纤维细胞 / 脂肪源干细胞 / 成骨分化
Fasciology / Retinoic acid / Fibroblasts / Adipose derived stem cells / Osteogenic differentiation
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