机械压应力通过TGF-β1通路治疗增生性瘢痕的分子机制研究

中国临床解剖学杂志 ›› 2013, Vol. 31 ›› Issue (3): 299-302.

机械压应力通过TGF-β1通路治疗增生性瘢痕的分子机制研究

申宽宏，　黄东，　吴伟炽，　黄永军，　牟勇

南方医科大学第三临床学院和南方医科大学非直属附属广东省第二人民医院，广州 510317

收稿日期:2013-03-04 出版日期:2013-05-25 发布日期:2013-06-06
通讯作者: 黄东, 主任医师，教授，博士生导师，E-mail:dong-177@　163.com E-mail:308905459@qq.com
作者简介:申宽宏（1984-）,男，在读硕士，主要从事创伤显微外科研究Tel:13631325086
基金资助:
国家自然科学基金（81071564）

Study on the molecular mechanism of mechanical compression treating hyperplastic scar via TGF-β 1 pathway

SHEN Kuan-hong, HUANG Dong, WU Wei-chi, HUANG Yong-jun, MOU Yong

The Third Clinical College of Southern Medical University and Guangdong No.2 Provincal People's Hospital，Guangzhou 510317, China

Received:2013-03-04 Online:2013-05-25 Published:2013-06-06

摘要/Abstract

摘要：

目的　通过机械压应力系统干预增生性瘢痕成纤维细胞，研究皮肤创伤后增生性瘢痕组织中TGF-β1通路中TGF-β1受体、smads和胶原蛋白的差异性表达，探究压应力治疗增生性瘢痕的分子机制。方法　体外培养增生性瘢痕细胞，应用RT-PCR方法比较机械性压应力干预组和空白对照组增生性瘢痕成纤维细胞内Smad3、Smad7和胶原蛋白表达差异。结果　根据RT-PCR结果，增生性瘢痕成纤维细胞系中在机械性压应力干预后，TGF-β1受体表达量增高，Smad3、 Smad7、CollagenⅠ表达量下降，差异有统计学意义（P<0.05），collagenⅢ表达两组差异无统计学意义（P>0.05）。结论　机械压应力治疗增生性瘢痕可通过TGF-β1通路中抑制Smad3的表达从而促进胶原蛋白Ⅰ的降解。

关键词: 增生性瘢痕, 成纤维细胞, 机械压应力, 胶原蛋白, 转化生长因子-&beta

Abstract:

Objective　To study the effects of mechanical compression on the expression of TGF-β1 receptor，smads and collagen from human skin hypertrophic scar fibroblast via the mechanical pressure system intervention, and explore the potential molecular mechanism of pressure treatment of hyperplastic scar. Methods　Hypertrophic scar cells were cultured in vitro ，the expression of TGF-β1 receptor，Smad3,Smad7 and collagens were determined on mRNA level by RT-PCR; Difference in expression between the mechanical pressure intervention experimental group and the control group in the hypertrophic scar fibroblasts was tested. Results　The RT - PCR results indicated that the expression of smad3 and smad7, Collagen Ⅰ was reduced after pressure treatment the difference being statistically significant (P<0.05), whereas the expression of TGF-β1 receptor increased, the difference being statistically significant (P<0.05).　Conclusion　Mechanical compression can inhibit Smad3 expression and promote TGF-β1 receptor expression so as to promote the degradation of hyperplastic scar collagen.

Key words: Hypertrophic scar, Fibroblast, Mechanical compression, TGF-β, Collagen

中图分类号:

R641

申宽宏, 黄东, 吴伟炽, 黄永军, 牟勇. 机械压应力通过TGF-β1通路治疗增生性瘢痕的分子机制研究[J]. 中国临床解剖学杂志, 2013, 31(3): 299-302.

SHEN Kuan-Hong, HUANG Dong, TUN Wei-Chi, HUANG Yong-Jun, MAO Yong. Study on the molecular mechanism of mechanical compression treating hyperplastic scar via TGF-β 1 pathway[J]. Chinese Journal Of Clinical Anatomy, 2013, 31(3): 299-302.

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