振动运动对大鼠骨骼肌AMPK-GLUT4糖代谢通路的影响
公小娟, 刘畅, 姜丁文, 梅晰凡, 房师强
中国临床解剖学杂志 ›› 2013, Vol. 31 ›› Issue (3) : 293-298.
振动运动对大鼠骨骼肌AMPK-GLUT4糖代谢通路的影响
Effects of vibration training on AMPK-GLUT4 passage of Rat skeletal muscle
目的 研究不同频率和时间的振动运动对大鼠骨骼肌糖代谢的影响，并探讨适宜的振动频率和振动时间。 方法 42只SD雄性大鼠，根据对其施加的不同振动频率（15、25、35 Hz）和持续时间（5 min和15 min）随机分成7组：安静对照组(CC) 、低频率短时间振动训练组(LS) 、低频率长时间振动训练组（LL）、中频率短时间振动训练组(MS) 、中频率长时间振动训练组（ML）、高频率短时间振动训练组(HS)和高频率长时间振动训练组( HL) 。每组进行相应频率和时间的振动运动，每周训练6 d，持续8周。训练结束后取材，检测空腹血糖、肌糖元、肌酸激酶和肌纤维横截面积;免疫组化、Western blot检测腺苷酸活化蛋白激酶（AMPK）和葡萄糖转运蛋白4（GLUT4）的表达。 结果 （1）与CC组相比，ML组血糖显著降低（P<0.05）,肌纤维横截面积显著减小（P<0.05），腓肠肌糖元、肌酸激酶增加幅度最大（P<0.05）。（2）与CC组相比，各实验组AMPK-GLUT4通路蛋白的表达显著高于CC组（P<0.05），其中ML组变化最明显，显著高于其它实验组（P<0.05）。 结论 （1）振动运动能够促进骨骼肌对葡萄糖的摄取利用，改善糖代谢。（2）中频率长时间的振动运动（25 Hz，15 min）最有利于促进大鼠骨骼肌细胞的糖代谢。
Objective To study the effects of different vibration training on glycometabolism of rat skeletal muscle, and further explore the suitable vibration training stimulus. Methods 42 male SD rats were divided randomly into 7 groups according to the vibration frequency (15, 25, 35 Hz) and time (5 min and 15 min) imposed：quiet control group(CC), Low-frequency vibration short group(LS), Low-frequency vibration prolonged group(LL), Medium-frequency vibration short time group(MS), Medium-frequency vibration prolonged time group(ML), High-frequency vibration short time group(HS) and High-frequency vibration prolonged group(HL). Vibration frequency is high frequency (35 Hz), Medium frequency (25 Hz) and low frequency (15 Hz), and training time is short (5 min) and prolonged (15 min), 6 days in one week, the experiment lasted for 8 weeks. After the last training bout (8 weeks), the rats were euthanized, and then the tissue samples were collected. The fasting blood-glucose in blood, muscle glycogen, muscle creatine kinase (CK) and muscle fiber cross-sectional area were analyzed. Adenosine Monophosphate Activated Protein Kinase (AMPK) and Glucose Transporter Four (GLUT4) were analyzed by immunohistochemistry and western blot. Results (1) Compared with the CC group, in the ML group, the fasting blood-glucose in blood was significantly lower, the muscle fiber cross-sectional area was significantly reduced, the muscle glycogen and muscle creatine kinase (CK) were significantly higher (P<0.05). (2) Compared with the CC group, the expression of ampk-glut4 in each group were significantly increased after vibration training, and the ML group was significantly higher than other experimental group (P<0.05). Conclusions (1) Vibration training can enhance the glycometabolism of Rat Skeletal Muscle. (2) The suitable vibration training stimulus (25 Hz,15 min) is helpful to increase the glycometabolism of rat skeletal muscle.
振动运动 / 骨骼肌 / 糖代谢 / AMPK-GLUT4
Vibrationtraining / Glycometabolism / Skeletal Muscle / Ampk-glut4
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国家自然科学基金项目（31100858）；辽宁省科技厅攻关技术项目（2012225019）
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