毛发生长周期对小鼠皮肤创伤愈合的影响

中国临床解剖学杂志 ›› 2013, Vol. 31 ›› Issue (3): 283-287.

毛发生长周期对小鼠皮肤创伤愈合的影响

孔亚男¹，　张敏¹，　崔梦卿²,　李良平²，　周志涛¹，　张琳¹

1. 南方医科大学组织胚胎学教研室，广州 510515； 2. 南方医科大学南方医院门诊部，广州 510515;
3.　广州市红十字会医院神经外科，广州 510220

收稿日期:2013-03-26 出版日期:2013-05-25 发布日期:2013-06-06
通讯作者: 张琳，教授，博士生导师，Tel: (020)61648205, E-mail:zlilyzh@126.com E-mail:kongyanan088@163.com
作者简介:孔亚男（1987-），女，安徽省滁州人，硕士，研究方向：皮肤的创伤修复，Tel: (020)62789373
基金资助:
国家自然科学基金（81171824，30973129）；广东市科技计划项目（12C32121608）；2011省高校人才引进专项（C1031118）；高等学校博士学科点专项科研基金（博导类20104433110009）；广东省自然科学基金（10151022001000003）

Impact of hair growth phase on mouse skin wound healing

KONG Ya-nan¹, ZHANG Min¹, CUI Meng-qing²，LI Liang-ping³, ZHOU Zhi-tao¹, ZHANG Lin¹

1.Department of Histology and Embryology, Southern Medical University, Guangzhou 510515, China; 2.Outpatient Department, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China; 3.Neurosurgery Department of Guangzhou Red Cross Hospital, Guangzhou 510220, China

Received:2013-03-26 Online:2013-05-25 Published:2013-06-06

摘要/Abstract

摘要：

目的　探讨处于不同毛发生长周期的C57BL/6小鼠皮肤创伤愈合速度。方法　制备小鼠皮肤创伤模型，计算术后0、3、7 d创面愈合率，愈合率=（原始创面面积－未愈合的创面面积）/原始创面面积×100%，比较毛发静止期（Hair telogen stages）小鼠和毛发生长期（Hair anagen stages）小鼠伤口愈合速度。采用HE染色比较伤口愈合的组织形态结构差异，利用BrdU检测伤口周围细胞增殖。结果　毛发生长期的小鼠皮肤伤口愈合率显著高于毛发静止期的小鼠伤口愈合率。HE染色显示毛发生长期小鼠伤口周围表皮细胞层较多，且表皮细胞向伤口迁移增强；BrdU检测显示毛发生长期小鼠皮肤伤口周围表皮BrdU+ 细胞数多于毛发静止期小鼠。结论　毛发生长期的小鼠皮肤创伤愈合率高于毛发静止期小鼠，这一结果为进一步探讨毛囊在创伤愈合过程中的作用提供研究基础，也为选择皮肤创伤愈合动物模型提供指导。

关键词: 创伤愈合, 毛发生长周期, 动物模型

Abstract:

Objective　To investigate the skin wound healing speed of C57BL/6 mice at various stages of hair growth cycle.　Methods　Mouse skin wounding models were established and wound healing rate in the following manner was calculated: Wound closure rate (%) = [(Original wound area - Open area on the final day)/Original wound area] ×100%. Wound healing speed of mouse at hair anagen stages and at hair telogen stages was compared. H&E staining was adopted to compare differences in morphological and histological characteristics of wound healing. BrdU was used to detect cell proliferation around wounds.　Results　Wound healing rate of mouse at hair anagen stages was significant higher than that of mouse at telogen stages. H&E staining showed that mice presented relatively more epidermal cell layers around wounds and epidermal cell layers migrated faster at hair anagen stages. BrdU detection showed that mice at hair anagen stages had a larger number of BrdU+ epidermal cells around wounds than mouse at telogen stages.　Conclusion　Mice at hair anagen stages enjoy a faster skin wound healing rate than mice at telogen stages, which provides a basis for further research into the role of hair follicle in wound healing process as well as a guidance on how to choose animal models for study of skin wound healing.

Key words: Wound healing, Hair growth cycle, Animal model

中图分类号:

孔亚男, 张敏, 崔梦卿, 李良平, 周志涛, 张琳. 毛发生长周期对小鼠皮肤创伤愈合的影响[J]. 中国临床解剖学杂志, 2013, 31(3): 283-287.

KONG E-Nan, ZHANG Min, CUI Meng-Qing, LI Liang-Beng, ZHOU Zhi-Chao, ZHANG Lin. Impact of hair growth phase on mouse skin wound healing[J]. Chinese Journal Of Clinical Anatomy, 2013, 31(3): 283-287.

参考文献

[1] 罗满林.实验动物学
[M].北京：中国农业出版社, 2002：87-90.

[2] 张敏,朱晓文,张琳等. C57BL/6小鼠皮肤毛囊发育的研究
[J]. 中国临床解剖学杂志, 2010, 28(1):74-77.

[3] 孙丽, 张敏, 张琳，等. Activin B联合BMSC不同移植方式对大鼠皮肤创伤愈合的治疗
[J]. 中国临床解剖学杂志, 2012, 30(2):197-202.

[4] Müller-R?ver SB, Handjiski C, van der Veen S, et al. A comprehensive guide for the accurate classification of murine hair follicles in distinct hair cycle stages
[J]. J Invest Dermatol, 2001, 117(1): 3-15.

[5] Zhang M, Liu NY, Wang XE, et al. Activin B promotes epithelial wound healing in vivo through RhoA-JNK signaling pathway
[J]. PLoS One, 2011, 6(9):e25143.

[6] 杨宗成.烧伤治疗学
[M].北京：人民卫生出版社, 2006：180-181.

[7] 邹仲之.组织学与胚胎学
[M]. 北京：人民卫生出版社, 2004：115-116.

[8] Lavker RM, Sun TT, Oshima H, et al. Hair follicle stem cells
[J]. Investig Dermatol Symp Proc, 2003, 8(1):28-38.

[9] Plikus MV, Gay DL, Treffeisen E, et al. Epithelial stem cells and implications for wound repair
[J]. Semin Cell Dev Biol, 2012, 23(9):946-953.

[10] Brownell I, Guevara E, Bai CB, et al. Nerve-derived sonichedgehog defines a niche for hair follicle stem cells capable of becoming epidermal stem cells
[J]. Cell Stem Cell, 2011, 8(5):552-565.

[11] Tiede S, Kloepper JE, Bodò E, et al. Hair follicle stem cells: walking the maze
[J]. Eur J Cell Biol, 2007, 86(7):355-376.

[12] Ito M, Cotsarelis G. Is the hair follicle necessary for normal wound healing
[J]. Invest Dermatol, 2008, 128(5):1059-1061.

[13] Qi SH, Liu P, Xie JL, et al. Experimental study on repairing of nude mice skin defects with composite skin consisting of xenogeneic dermis and epidermal stem cells and hair follicle dermal papilla cells
[J]. Burns, 2008, 34(3):385-392.