改良阴茎海绵体压力测定方法在建立糖尿病性ED大鼠模型中的应用研究
吴子云, 何书华, 张涛, 刘路浩, 韦安阳
中国临床解剖学杂志 ›› 2013, Vol. 31 ›› Issue (2) : 192-195.
改良阴茎海绵体压力测定方法在建立糖尿病性ED大鼠模型中的应用研究
Research for modification of intracavernous pressure monitoring in establishing rat model with diabetic erectile dysfunction
目的 评价改良阴茎海绵体压力（intracavernous pressure, ICP）测定方法在建立糖尿病性勃起功能障碍（erectile dysfunction, ED）大鼠模型中的应用。 方法 将40只8周龄雄性SD大鼠随机分为糖尿病组及正常对照组，检测大鼠ICP及颈动脉压力（arterial pressure, AP），改良法采用带倒刺的穿刺针刺入阴茎脚。 结果 在糖尿病组及正常对照组中，与传统方法比较，采用改良阴茎海绵体压力测定方法所测的ICP与AP结果均无统计学意义（P ＞ 0.05），但改良法穿刺针不易脱出，保证了实验顺利进行，成功率大大提高（传统法成功率75%，改良法100%）。 结论 改良的带倒刺的穿刺针刺入阴茎脚测定大鼠阴茎海绵体压力具有稳定、准确、方便和不易脱出的特点，对筛选糖尿病性ED大鼠动物模型、评价大鼠阴茎勃起功能具有重要意义。
Objective To evaluate the modified method of intracavernous pressure(ICP) monitoring in establishing rat model with diabetic erectile dysfunction. Methods Forty male rats were randomized equally into 2 groups (control group and diabetic group). ICP and arterial pressure (AP) were monitored with the improved method and the traditional method, respectively. The modified method used improved intravenous infusion needle puncture in the crus of penis. Results There was no significant difference in measurement of the ICP and AP between the modified method and the traditional method（P＞0.05）, both in the control group and diabetic group. The modified method used improved intravenous infusion needle puncture in the crus of penis can improve the success rate significantly (the traditional method being 75% and the modified method being 100%). Conclusion The modified method of intracavernous pressure monitoring has characteristic of stable、accurate、higher success rate. It is of great significance for establishing rat model with diabetic erectile dysfunction and evaluating rat penile erectile function.
改良 / 阴茎海绵体压力 / 方法 / 勃起功能障碍 / 糖尿病
Modification / Intracavernous pressure / Method / Erectile dysfunction / Diabetic
[1] Ponholzer A, Temml C, Mock K, et al. Prevalence and risk factors for erectile dysfunction in 2869 men using a validatedquestionnaire
[J]. Eur Urol,2005,47: 80-86.
[2] Malavige LS, Levy JC. Erectile dysfunction in diabetes mellitus
[J].J Sex Med, 2009, 6(5):1232-1247.
[3] Cho NH, Ahn CW, Park JY, et al. Prevalence of erectile dysfunction in Korean men with Type 2 diabetes mellitus
[J]. Diabet Med, 2006, 23(2):198-203.
[4] Chen KK, Chan JY, Chang LS, et al. Intracavernous pressure as an experimental index in a rat model for the evaluation of penile erection
[J]. J Urol, 1992,147(4):1124-1128.
[5] Sezen, SF, Burnett AL. Intracavernosal pressure monitoring in mice: responses to electrical stimulation of the cavernous nerve and to intracavernosal drug administration
[J]. J Androl, 2000, 21(2): 311-315.
[6] Qiu X, Villalta J, Ferretti L, et al. Effects of intravenous injection of adipose-derived stem cells in a rat model of radiation therapy-induced erectile dysfunction
[J]. J Sex Med, 2012, 9(7): 1834-1841.
[7] Chen Y, Li XX, Lin HC,et al. The effects of long-term administration of tadalafil on STZ-induced diabetic rats with erectile dysfunction via a local antioxidative mechanism
[J]. Asian J Androl, 2012,14(4): 616-620.
[8] 程祎,韦安阳,李煜罡.糖尿病性勃起功能障碍大鼠模型的建立
[J].南方医科大学学报，2008，28(4)：564-566.
[9] Lawa M. Management of diabetes mellitus in clinical practice
[J]．Br J Nurs，2008，17(17)：1106-1113.
[10] Yamashita S, Kato R, Kobayashi K, et al. Nerve injury-related erectile dysfunction following nerve-sparing radical prostatectomy a novel experimental dissection model
[J]. Int J Urol, 2009, 16(11), 905–911.
[11] 张新华,胡礼泉,陈坚,等.海绵体神经损伤与阴茎勃起功能障碍
[J].中华泌尿外科杂志, 2002, 23(11):702-703.
[12] 邢俊平,崔险峰,孙建华, 等.电刺激阴茎背神经和海绵体内注射药物诱导大鼠阴茎海绵体内压反应
[J].中华男科学杂志，2005, 11(4):281-283.
[13] 李铮.电刺激诱导阴茎勃起的研究
[J].国外医学：泌尿系统分册, 2000, 20(6):280-281.
[14] Andersson KE，Wagner G．Physiology of penile erection
[J]．Physiol Rev，1995，75(1)：191-236．
[15] Hellstrom WJG．The handbook of sexum dysfunction
[M]．San Francisco：The American Society of Andrology，1999:12-17．
[16] 崔险峰, 张云山, 邢俊平, 等. 阴茎勃起动物模型的建立和检测研究进展
[J].山西医药杂志，2006, 35(10):907-908.
国家自然科学基金（81170566）
京ICP备08002354号-3/
| 〈 |
|
〉 |
