同种异体BMSCs、ADSCs移植后的抗原性差异

李磊; 秦书俭; 包翠芬; 马刚; 李季; 刘宇卓

中国临床解剖学杂志 ›› 2013, Vol. 31 ›› Issue (2) : 174-179.

实验研究

李磊，　秦书俭，　包翠芬，　马刚，　李季，　刘宇卓

作者信息 +

Antigenic differences of allogeneic BMSCs，ADSCs after transplantation

LI Lei，QIN Shu-jian，BAO Cui-fen，MA Gang, LI Ji，LIU Yu-zhuo

Author information +

文章历史 +

摘要

目的　探讨骨髓来源间充质干细胞（MSCs from bone marrow， BMSCs）与脂肪来源间充质干细胞(Adipose tissue-derived MSCs，ADSCs)体内移植后的抗原性差异。方法体外培养WKY大鼠BMSCs、ADSCs，经成骨诱导后植入wistar大鼠桡骨缺损区，分别于植入后1、2、4、8周进行缺损区HE染色，于1、2、4、8周免疫组化检测缺损区IL-2、TGF-β、TNF-α、CD4、CD8，采用ELISA法检测脾细胞培养液上清IL-2、TGF-β、TNF-α的含量。结果 BMSCs、ADSCs移植术后1～2周有少量淋巴细胞、白细胞浸润，IL-2、TGF-β、TNF-α、CD4、CD8蛋白少量表达；二者比较差异无显著性。结论 BMSCs、ADSCs体内移植后所致的抗原性无显著性差异。

Abstract

Objective to investigate the antigenic difference of allogeneic bone marrow-derived mesenchymal stem cells( BMSCs) and adipose tissue-derived mesenchymal cells (ADSCs) after their transplantation. Method BMSCs, ADSCs from WKY rats were cultured in vitro and were transplanted into the radial osseous defects in Wistar rats after osteogenic induction; 1、2、4、8 weeks after implantation, tissues from the defective areas was taken for HE staining, IL-2, TGF- β, TNF- α, CD4, CD8 were detected using immunohistochemical staining and quantity of IL-2, TGF- β, TNF- α was determined in supernatant of spleen cell culture using ELISA. Results 1~2 weeks after transplantation of BMSCs, ADSC, mild lymphocyte, leukocyte infiltration can be observed in the defective areas, a small amount of IL-2, TGF- β, TNF- α, CD4, CD8 can be detected; there was no significant difference in the 2 groups. Conclusion No significant difference can be found in BMSCs, ADSCs after transplantation.

导出引用

李磊, 秦书俭, 包翠芬, 马刚, 李季, 刘宇卓. 同种异体BMSCs、ADSCs移植后的抗原性差异[J]. 中国临床解剖学杂志. 2013, 31(2): 174-179

LI Lei, QIN Shu-Jian, BAO Cui-Fen, MA Gang, LI Ji, LIU Yu-Zhuo. Antigenic differences of allogeneic BMSCs，ADSCs after transplantation[J]. Chinese Journal of Clinical Anatomy. 2013, 31(2): 174-179

中图分类号： R392.12

参考文献

[1] Le Blanc K,Tammik L,Sundberg B,et al. Mesenchymal stem cells inhibit and stimulate mixed lymphocyte cultures and mitogenic responses independently of the major histocompatibility complex
[J]. Scand J Immunol,2003, 57(1): 11-20.

[2] Bartholomew A,Sturgeon C,Siatskas M,et al.Mesenchymal stem cells suppress lymphocyte proliferation in vitro and prolong skin graft survival in vivo
[J]．Exp Hematol,2002,30(1)：42-48.

[3] 马刚,秦书俭,包翠芬,等.大鼠ADSCs、BMSCs、PMSCs体外诱导成骨样细胞的抗原性差异
[J]. 中国医学工程,2012,20（8）：34-36.

[4] Crop MJ, Baan CC, Korevaar SS, et al. Donor-derived mesenchymal stem cells suppress alloreactivity of kidney transplant patients. Transplantation. 2009;87(6):896-906.

[5] Kuo YR, Chen CC, Goto S, et al. Modulation of immune response and T-cell regulation by donor adi pose-derived stem cells in a rodent hindlimb allotransplant model. Plast Reconstr Surg, 2011, 128(6): 661e-672e.

[6] Kang MI, Lee WY, KW, et al．The short-term changes of boneminerM metabolism folowing bone man'ow transplantation
[J]．Bone, 2000, 26(3)：275-279.

[7] Sioud M, Mobergslien A, Boudabous A, et al. Evidence for the involvement of galectin-3 in mesenchymal stem cell suppression of allogeneic T-cell proliferation. Scand J Immunol, 2010, 71(4): 267-274.

[8] Kotobuki N, Katsube Y, Katou Y,et al. In vivo survival and osteogenic differentiation of allogeneic rat bone marrow mesenchymal stem cells (MSCs)
[J].Cell Transplant, 2008,17(6):705-712.

[9] Noel D, Djouad F, Bouffi C, et al. Multipotent mesenchymal stromal cells and immune tolerance
[J]. Leuk Lymphoma, 2007, 48(7):1283-1289.

[10]Ren G, Su J, Zhang L, et al. Species variation in the mechanisms of mesenchymal stem cell-mediated immunosuppression
[J]. Stem Cells, 2009, 27(8): 1954-1962.