皮质发育障碍大鼠海马神经元的体视学方法定量研究

冯飞; 陈筱山; 贺兴; 张建刚; 文明; 唐勇; 晏勇

中国临床解剖学杂志 ›› 2013, Vol. 31 ›› Issue (1) : 85-88.

实验研究

冯飞¹，陈筱山¹，　贺兴¹，　张建刚¹，　文明¹，　唐勇²，　晏勇¹

作者信息 +

Quantitative study of variation of number of neurons in cortical dysplasia rat hippocampus by stereological method

FENG Fei¹, CHEN Xiao-shan¹, HE Xing¹, ZHANG Jian-gang¹, WEN Ming¹, TANG Yong², YAN Yong¹

Author information +

文章历史 +

摘要

目的　用体视学方法计数比较X射线制作的大脑皮质发育障碍（DCDs）模型大鼠海马神经元数量变化和测量海马体积变化。方法　选择2月龄正常SD大鼠和DCDs模型鼠各5 只，取脑后石蜡包埋、连续冠状切片。根据均匀系统随机抽样原则，从含海马结构的切片中随机抽取一组切片，组织化学染色，在体视学设备下计数大鼠海马CA1、CA3和DG区的神经元数目和测量海马体积。结果　正常大鼠单侧海马CA1、CA3和DG区的神经元的数目分别是（8.35×104±1.44×104）、（8.23×104±1.60×104）和（1.43×105±2.24×104），DCDs模型大鼠单侧海马CA1、CA3和DG区的神经元的数目分别是（3.70×104±　1.96×104）、（3.57×104±1.47×104）和（6.86×104±4.85×104）；正常大鼠和DCDs模型鼠单侧海马的体积分别是(14.18±1.52)mm3和(8.49±3.41)mm3。DCDs大鼠海马结构各亚区的神经元数量和海马体积均较正常鼠明显减小（P＜0.05）。结论　X射线制作的DCDs模型鼠海马CA1、CA3和DG的神经元数目和海马体积都较正常大鼠减少。

Abstract

Objective　To investigate the variation of number and volume of hippocampus neuron in CA1,CA3 and DG region of rats with cortical dysplasia induced by X-ray using stereological method. Methods 5 normal rats and 5 model rats with 2 months old were used in this study with their brain subsequently and sequentially taken out, embedded in paraffin blocks and being subject to coronal section in thickness of 8 ?滋m. The sections were uniformly and randomly sampled from the consecutive sections of the hippocampus according to the principles of systematic，uniform and random sampling．The sections were stained for Nissl substance for quantification of neuron number in CA1，CA3 ,DG region and volume of the hippocampus using stereological device. Results The number of unilateral hippocampus neurons in CA1，and DG region in normal rats was 8.35×104±1.44×104、8.23×104±1.60×104 and 1.43×105±2.24×104, respectively，and was 3.70×104±1.96×104、3.57×104±1.47×104 and 6.86×104±4.85×104 in model rats with cortical dysplasia, respectively. The volume of the unilateral hippocampus in normal rats and rats with disorders of cortical development was (14.18±1.52) mm3 and (8.49±3.41) mm3, respectively. Compared to normal rats, the number of neurons in CA1,CA3 and DG region and the volume of hippocampus in model rats decreased(P<0.05).　Conclusion　Compared with normal rats, the number and volume of hippocampus neuron in CA1, CA3 and DG regions of rats with cortical dysplasia induced by X-ray is significantly reduced.

导出引用

冯飞, 陈筱山, 贺兴, 张建刚, 文明, 唐勇, 晏勇. 皮质发育障碍大鼠海马神经元的体视学方法定量研究[J]. 中国临床解剖学杂志. 2013, 31(1): 85-88

FENG Fei, CHEN Xiao-Shan, HE Xin, ZHANG Jian-Gang, WEN Meng, TANG Yong, YAN Yong. Quantitative study of variation of number of neurons in cortical dysplasia rat hippocampus by stereological method[J]. Chinese Journal of Clinical Anatomy. 2013, 31(1): 85-88

中图分类号： R741.02

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