NG2细胞在大鼠脊髓压迫性损伤急性期内源性增殖及形态变化规律

漆伟; 孙善全; 冉建华; 黄思琴; 伍修宇; 侯良绢; 卓飞; 陈臻

中国临床解剖学杂志 ›› 2013, Vol. 31 ›› Issue (1) : 81-84.

实验研究

漆伟¹，　孙善全¹，　冉建华¹，　黄思琴¹，　伍修宇¹，　侯良绢¹，　卓飞¹，　陈臻²

作者信息 +

Endogenous proliferation and morphological characteristics of NG2-cells in compressed spinal cord injury of rats

QI Wei¹, SUN Shan-quan¹, RAN Jian-hua¹, HUANG Siqin¹, WU Xiu-yu¹, HOU Liang-juan¹, ZHUO Fei¹, CHEN Zhen²

Author information +

文章历史 +

摘要

目的　研究NG2细胞在大鼠脊髓损伤白质内源性增殖及形态特征。方法　成年SD雄性大鼠42只，随机平均分为模型组和假手术组。按课题组自行设计的方法制作脊髓压迫模型，假手术组仅暴露脊髓。分别于术后1、3、7d运用免疫组化检测脊髓内NG2细胞的表达。采用Image Pro Plus6.0软件对NG2阳性细胞计数并测量其胞体面积和突起长度。结果　伤后1d，NG2+细胞增多（30.17±11.08）/视野，至3d达到高峰(90.75±9.40) /视野，7d后下降（78.38±8.91）/视野，但仍多于假手术组（19.92±6.68）/视野（P＜0.05）。在假手术组，NG2+细胞平均胞体面积为（205.67±10.80）μm2、平均突起长度为（22.92±1.24）μm，伤后1d，NG2+细胞胞体变小（128.25±32.06）μm2、突起变短(10.98±4.25) μm，3d后胞体变大(225.26±　16.64) μm2、突起增长(18.63±2.26) μm（P＜0.05），至7d变化不明显（P＞0.05）。在脊髓压迫损伤后，可见许多胞体较小呈圆形、突起少或无的NG2+细胞集落。结论　在脊髓压迫损伤一周内，NG2细胞增殖活跃，胞体渐大，突起变长，但仍短于正常。

Abstract

　Objective　To investigate endogenous proliferation and morphological characteristics of NG2-cells in the white matter of compressed spinal cord injury (ACSCI) of rats.　Methods　42 adult SD rats were randomly divided into 2 groups: model group (n=21), which underwent spinal cord compression by a self-made device, and sham group (n=21), which underwent only laminectomy. Expression of NG2-positive cells was detected by IHC after 1, 3 and 7 days. The average number, soma area, process length of NG2+-cells were measured by Image Pro Plus6.0.　Results　Compared with control group (19.92±6.68 per field), the number of NG2+-cells increased at 1d (30.17±11.08 per field), peaked at 3d (90.75±9.40 per field), and decreased at 7d (78.38±8.91 per field) (P＜0.05). NG2+-cells' soma became smaller (128.25±32.06 μm2) and processes became shorter (10.98±4.25 μm) at 1d, then NG2+-cells' soma became larger (225.26±16.64 μm2) and processes became longer (18.63±2.26 μm) at 3d (P＜0.05). However, the soma area and process length of NG2+-cells at 7d kept unchanged compared with that at 3d (P＞0.05). There were many clusters of NG2+ cells that had smaller and round soma, fewer or no processes after CSCI.　Conclusion　NG2+-cells could proliferate actively with the soma growing larger and the process growing longer but still shorter than normal in CSCI after a week.

导出引用

漆伟, 孙善全, 冉建华, 黄思琴, 伍修宇, 侯良绢, 卓飞, 陈臻. NG2细胞在大鼠脊髓压迫性损伤急性期内源性增殖及形态变化规律[J]. 中国临床解剖学杂志. 2013, 31(1): 81-84

QI Wei, SUN Shan-Quan, DAN Jian-Hua, HUANG Sai-Qin, WU Xiu-Yu, HOU Liang-Juan, ZHUO Fei, CHEN Zhen. Endogenous proliferation and morphological characteristics of NG2-cells in compressed spinal cord injury of rats[J]. Chinese Journal of Clinical Anatomy. 2013, 31(1): 81-84

中图分类号： R741.05

参考文献

[1] 谷华运, 周国民. 神经系统的组织学和细胞学
[M]. 上海: 复旦大学出版社, 2007:100-101.

[2] Peters A. A fourth type of neuroglial cell in the adult central nervous system
[J]. J Neurocytol, 2004, 33(3):345-357.

[3] 梁益建, 孙善全, 汪克建, 等. 大鼠脊髓慢些压迫性损伤实验模型的建立
[J]. 中国临床解剖学杂志, 2006, 24(03):320-324.

[4] 赵淑敏, 孔祥玉, 周建, 等. 黄芪预处理对缺血再灌注大鼠心肌超微结构的保护作用
[J]. 中国临床康复杂志, 2005, 9(27):80-81.

[5] Ganji F, Behzadi G. Postnatal development of masseteric motoneurons in congenital hypothyroid rats
[J]. Brain Res, 2007, 1129(1):81-88.

[6] Mekhail M, Guillermina A, Tabrizian M. Oligodendrocyte-protection and remyelination post-spinal cord injuries: A review
[J]. Progress in Neurobiology, 2012, 96(3):322-339.

[7] 伍亚民, 李民, 廖维宏. 脊髓损伤修复的研究现状及存在的问题［J］. 中国矫形外科杂志, 2004, 12(14):1097-1099.

[8] Nishiyama A, Yang Z, Butt A. Astrocytes and NG2-glia: what’s in a name
[J]? J Anat, 2005, 207(6):687-694.　

[9] Mangin JM, Gallo V. The curious case of NG2 cells: transient trend or game changer
[J] ? Asn Neuro,2011, 3(1):37-49.

[10] Wigley R, Butt AM. Integration of NG2-glia (synantocytes) into the neuroglial network
[J]. Neuron Glial Biol, 2009, 5(1-2):21-28.

[11] Trotter J, Khalad K, Nishiyama A. NG2 cells: Properties, progeny and origin
[J]. Brain Res Rev, 2010, 63(1-2):72-82.

[12] Tong XP, Li XY, Zhou B, et al. Ca2+ signaling evoked by activation of Na+ channels and Na+／ca2+ exchangers is required for GABA induced NG2 cell migration
[J]. J Cell Biol, 2009, 186(1):113-128.

[13] Bergles DE, Jabs R, Steinh?覿user C. Neuron-glia synapses in the brain
[J]. Brain Res Rev, 2010,63(1-2):130-137.

[14]Yang ZS, Suzuki R, Daniels SB, et al. NG2 glial cells provide a favorable substrate for growing axons
[J]. J Neurosci, 2006, 26(14):3829-3839.

[15] Nishiyama A. Polydendrocytes: NG2 cells with many roles in development and repair of the CNS
[J]. Neuroscientist, 2007,13(1):62-76.

[16] Miron VE, Kuhlmann T, Antel JP. Cells of the oligdendroglial lineage, myelination, and remyelination
[J]. Biochim Biophys Acta, 2011,1812(2):184-193.

[17] 李文娟，陈惠金，钱龙华，等.缺血启动未成熟大鼠脑白质内源性修复功能的研究
[J].中国当代儿科杂志，2012,14（7）：548-553.