NG2细胞在大鼠脊髓压迫性损伤急性期内源性增殖及形态变化规律

中国临床解剖学杂志 ›› 2013, Vol. 31 ›› Issue (1): 81-84.

NG2细胞在大鼠脊髓压迫性损伤急性期内源性增殖及形态变化规律

漆伟¹，　孙善全¹，　冉建华¹，　黄思琴¹，　伍修宇¹，　侯良绢¹，　卓飞¹，　陈臻²

1.重庆医科大学神经科学研究中心, 重庆 400016；　2.云南省第一人民医院眼科, 云南 650032

收稿日期:2012-08-02 发布日期:2013-01-29
通讯作者: 孙善全，教授，博士生导师，E-mail：sunsq2151@sohu.com E-mail:qiwei_911@163.com
作者简介:漆伟（1987-），男，江西宜春人，在读硕士，主要从事神经生物学研究，Tel:18716469705
基金资助:
国家自然科学基金(30270437)

Endogenous proliferation and morphological characteristics of NG2-cells in compressed spinal cord injury of rats

QI Wei¹, SUN Shan-quan¹, RAN Jian-hua¹, HUANG Siqin¹, WU Xiu-yu¹, HOU Liang-juan¹, ZHUO Fei¹, CHEN Zhen²

1.Institute of Neuroscience, Chongqing Medical University, Chongqing 40016; 2.Department of Ophthalmology, The first People's Hospital of Yunnan, Kunming 650032, China

Received:2012-08-02 Published:2013-01-29

摘要/Abstract

摘要：

目的　研究NG2细胞在大鼠脊髓损伤白质内源性增殖及形态特征。方法　成年SD雄性大鼠42只，随机平均分为模型组和假手术组。按课题组自行设计的方法制作脊髓压迫模型，假手术组仅暴露脊髓。分别于术后1、3、7d运用免疫组化检测脊髓内NG2细胞的表达。采用Image Pro Plus6.0软件对NG2阳性细胞计数并测量其胞体面积和突起长度。结果　伤后1d，NG2+细胞增多（30.17±11.08）/视野，至3d达到高峰(90.75±9.40) /视野，7d后下降（78.38±8.91）/视野，但仍多于假手术组（19.92±6.68）/视野（P＜0.05）。在假手术组，NG2+细胞平均胞体面积为（205.67±10.80）μm2、平均突起长度为（22.92±1.24）μm，伤后1d，NG2+细胞胞体变小（128.25±32.06）μm2、突起变短(10.98±4.25) μm，3d后胞体变大(225.26±　16.64) μm2、突起增长(18.63±2.26) μm（P＜0.05），至7d变化不明显（P＞0.05）。在脊髓压迫损伤后，可见许多胞体较小呈圆形、突起少或无的NG2+细胞集落。结论　在脊髓压迫损伤一周内，NG2细胞增殖活跃，胞体渐大，突起变长，但仍短于正常。

关键词: 脊髓压迫伤, NG2细胞, 内源性增殖, 细胞形态

Abstract:

　Objective　To investigate endogenous proliferation and morphological characteristics of NG2-cells in the white matter of compressed spinal cord injury (ACSCI) of rats.　Methods　42 adult SD rats were randomly divided into 2 groups: model group (n=21), which underwent spinal cord compression by a self-made device, and sham group (n=21), which underwent only laminectomy. Expression of NG2-positive cells was detected by IHC after 1, 3 and 7 days. The average number, soma area, process length of NG2+-cells were measured by Image Pro Plus6.0.　Results　Compared with control group (19.92±6.68 per field), the number of NG2+-cells increased at 1d (30.17±11.08 per field), peaked at 3d (90.75±9.40 per field), and decreased at 7d (78.38±8.91 per field) (P＜0.05). NG2+-cells' soma became smaller (128.25±32.06 μm2) and processes became shorter (10.98±4.25 μm) at 1d, then NG2+-cells' soma became larger (225.26±16.64 μm2) and processes became longer (18.63±2.26 μm) at 3d (P＜0.05). However, the soma area and process length of NG2+-cells at 7d kept unchanged compared with that at 3d (P＞0.05). There were many clusters of NG2+ cells that had smaller and round soma, fewer or no processes after CSCI.　Conclusion　NG2+-cells could proliferate actively with the soma growing larger and the process growing longer but still shorter than normal in CSCI after a week.

Key words: Compressed spinal cord injury, , NG2 cells, , Endogenous proliferation, , Cellular morphology

中图分类号:

R741.05

漆伟, 孙善全, 冉建华, 黄思琴, 伍修宇, 侯良绢, 卓飞, 陈臻. NG2细胞在大鼠脊髓压迫性损伤急性期内源性增殖及形态变化规律[J]. 中国临床解剖学杂志, 2013, 31(1): 81-84.

QI Wei, SUN Shan-Quan, DAN Jian-Hua, HUANG Sai-Qin, WU Xiu-Yu, HOU Liang-Juan, ZHUO Fei, CHEN Zhen. Endogenous proliferation and morphological characteristics of NG2-cells in compressed spinal cord injury of rats[J]. Chinese Journal Of Clinical Anatomy, 2013, 31(1): 81-84.

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