侧颅底水平位火棉胶断层数据集获取及与CT影像对照观察

中国临床解剖学杂志 ›› 2013, Vol. 31 ›› Issue (1): 51-55.

侧颅底水平位火棉胶断层数据集获取及与CT影像对照观察

李希平¹，　夏寅²

1.首都医科大学附属北京安贞医院耳鼻咽喉头颈外科, 北京 100029; 　2.首都医科大学附属北京同仁医院耳鼻咽喉头颈外科, 北京 100730

收稿日期:2012-08-28 发布日期:2013-01-29
通讯作者: 夏寅，教授，Tel:65288432， E-mail:xiayin3@163.com E-mail:lxpent@163.com
作者简介:李希平（1970-）男，山东人，医学博士，副主任医师，主要从事耳外科及颅底外科的临床与解剖研究，Tel:(010)64456641

Acquisition of digital dataset of celloidin axial sectional images of temporal bone and comparative observation with CT images

LI Xi-ping¹, XIA Yin²

1.Department of Otorhinolaryngology Head and Neck Surgery, Affiliated Bijing Anzhen Hospital of Capital University of Medical Sciences, Beijing 100029, China; 2. Department of Otorhinolaryngology Head and Neck Surgery, Affiliated Bijing Tongren Hospital of Capital University of Medical Sciences, Beijing 100730, China

Received:2012-08-28 Published:2013-01-29

摘要/Abstract

摘要：

目的　制作侧颅底火棉胶水平位断层连续薄层切片,获取数字化图片库,为影像学断层研究及侧颅底疾病临床诊断提供参考。方法用2例尸头标本进行螺旋CT扫描后制作侧颅底区标本。火棉胶包埋后，用德国产大型轮式切片机沿水平位切片，厚度100 μm，每切一片均采用Sony F-717数码照相机（500万像素）摄影并保存在计算机。将断层照片与HE染色照片和CT影像进行对照观察，选取代表性层面进行描述,包括外半规管层面,面神经水平段层面,前庭窗层面,圆窗层面。结果共获取数字化连续图片2套,分别为300、340张，断层图像分辨率1920×2560 像素，结构毗邻关系显示清晰。与HE照片和CT片对照，可以更清楚地定位断层结构。结论火棉胶包埋技术是制作侧颅底大切片，获取高分辨率的数字化图片的理想方法，对颞骨内解剖结构的毗邻关系显示清晰，组织学与CT对照观察是研究侧颅底水平位断层解剖特点的重要方法, 对指导影像学诊断具有重要参考价值。

关键词: 侧颅底, 火棉胶, 解剖, 断层, 水平位

Abstract:

Objective　To make sequential axial sectional slices of the lateral skull base and obtain digitized dataset of the lateral skull base in order to provide reference for sectional study of imaging and clinical diagnosis of lateral skull base diseases.　Methods　Two cadaveric heads underwent coronal section after spiral CT scanning. After celloidin embedding, a brain slicer was used to make axial thin slices (100 μm), which was then photographed with a Sony F-717 and saved in a computer representative sections were selected for HE staining. Then the digital photo, the HE staining picture and the CT image were compared to describe the characteristics of anatomical structures, including the common crus plane, lateral semicircular canal plane, the horizontal segment of facial nerve plane, the vestibular window plane, the round window plane, internal orifice of jugular foramen plane.　Results　Two sets of complete axial digital thin sectional data were obtained (300 and 340 images, respectively). Delicate structures were shown clearly and spatial relationships between anatomical structures can be understood deeply by sequential, histological and CT comparative observation.　Conclusion　Celloidin embedding is an important method for obtaining high resolution sequential digital imaging data of lateral skull base. Histological and CT image comparative observation is an ideal method for studying characteristics of sectional anatomy of the lateral skull base. It provides valuble reference for imaging diagnosis.

Key words: Skull base, Lateral, Celloidin, Anatomy, ,Section, Axial

中图分类号:

R322

李希平, 夏寅. 侧颅底水平位火棉胶断层数据集获取及与CT影像对照观察[J]. 中国临床解剖学杂志, 2013, 31(1): 51-55.

LI Xi-Beng, JIA Yin. Acquisition of digital dataset of celloidin axial sectional images of temporal bone and comparative observation with CT images [J]. Chinese Journal Of Clinical Anatomy, 2013, 31(1): 51-55.

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