侧颅底水平位火棉胶断层数据集获取及与CT影像对照观察
Acquisition of digital dataset of celloidin axial sectional images of temporal bone and comparative observation with CT images
目的 制作侧颅底火棉胶水平位断层连续薄层切片,获取数字化图片库,为影像学断层研究及侧颅底疾病临床诊断提供参考。 方法 用2例尸头标本进行螺旋CT扫描后制作侧颅底区标本。火棉胶包埋后，用德国产大型轮式切片机沿水平位切片，厚度100 μm，每切一片均采用Sony F-717数码照相机（500万像素）摄影并保存在计算机。将断层照片与HE染色照片和CT影像进行对照观察，选取代表性层面进行描述,包括外半规管层面,面神经水平段层面,前庭窗层面,圆窗层面。 结果 共获取数字化连续图片2套,分别为300、340张，断层图像分辨率1920×2560 像素，结构毗邻关系显示清晰。与HE照片和CT片对照，可以更清楚地定位断层结构。 结论 火棉胶包埋技术是制作侧颅底大切片，获取高分辨率的数字化图片的理想方法，对颞骨内解剖结构的毗邻关系显示清晰，组织学与CT对照观察是研究侧颅底水平位断层解剖特点的重要方法, 对指导影像学诊断具有重要参考价值。
Objective To make sequential axial sectional slices of the lateral skull base and obtain digitized dataset of the lateral skull base in order to provide reference for sectional study of imaging and clinical diagnosis of lateral skull base diseases. Methods Two cadaveric heads underwent coronal section after spiral CT scanning. After celloidin embedding, a brain slicer was used to make axial thin slices (100 μm), which was then photographed with a Sony F-717 and saved in a computer representative sections were selected for HE staining. Then the digital photo, the HE staining picture and the CT image were compared to describe the characteristics of anatomical structures, including the common crus plane, lateral semicircular canal plane, the horizontal segment of facial nerve plane, the vestibular window plane, the round window plane, internal orifice of jugular foramen plane. Results Two sets of complete axial digital thin sectional data were obtained (300 and 340 images, respectively). Delicate structures were shown clearly and spatial relationships between anatomical structures can be understood deeply by sequential, histological and CT comparative observation. Conclusion Celloidin embedding is an important method for obtaining high resolution sequential digital imaging data of lateral skull base. Histological and CT image comparative observation is an ideal method for studying characteristics of sectional anatomy of the lateral skull base. It provides valuble reference for imaging diagnosis.
Skull base, Lateral / Celloidin / Anatomy / ,Section / Axial
[1] Parlier CC, Champsaur P, Perrin E，et al. High-resolution computed tomography of the canals of the temporal bone : anatomic correlations
[J]. Surg Radiol Anat, 1998, 20(6) :437-444.
[2] 靳颖, 刘津平, 田德润, 等. 颞骨的断面解剖学研究及其临床意义
[J]. 中国临床解剖学杂志, 2004, 22(4):353-356.
[3] 杨开明．医学影像学的发展与断层解剖学的未来
[J]．实用医学影像杂志，2002，3(4)：295-296
[4] Hayran M, Onerci M, Ozturk C.Evaluation of the temporal bone by anatomic sections and computed tomography
[J]. Surg Radiol Anat,1992,14(2):169-173.
[5] Harada T, Ishii S, Tayama N.Three-dimensional reconstruction of the temporal bone from histologic sections
[J].Arch Otolaryngol Head Neck Surg,1988,114(10):1139-1142.
[6] Dai P, Liu Y, Jiang SC, et al. Stereo morphology of temporal bone and ear
[J].Chin Med J (Engl), 2004, 117(5):733-737.
[7] Qiu MG. Zhang SX, Liu ZJ, et al. Plastination and computerized 3D reconstruction of the temporal bone
[J]. Clin Anat, 2003, 16(4):300-303.
[8] 李希平，夏寅，韩德民. 基于虚拟中国人数据集的鼻部及颞骨解剖结构三维重建
[J].中国临床解剖学杂志, 2004, 22(4)：377-379.
[9] S?rensen MS, Dobrzeniecki AB, Larsen P, et al. The visible ear: a digital image library of the temporal bone
[J]. ORL J Otorhinolaryngol Relat Spec, 2002 ,64(6):378-381.
[10]李希平，韩德民，夏寅.基于颞骨冰冻切片数据集的侧颅底区解剖结构三维重建
[J].中国耳鼻咽喉头颈外科, 2007, 14(3)：149-151.
[11]Wang H, Merchant SN,?Sorensen?MS. A downloadable three-dimensional virtual model of the visible ear
[J].ORL J Otorhinolaryngol Relat Spec,2007,69(2):63-67.
[12]Cooper MH, Archer CR, Kveton JF. Correlation of high-resolution computed tomography and gross anatomic sections of the temporal bone:part I.The facial nerve
[J]. Am J Otol,1987,8(5):375-384.
[13]Cooper MH, Archer CR, Kveton JF.Correlation of high resolution computed tomography and gross anatomic sections of the temporal bone. Part III. Cochlear and vestibular aqueducts
[J].Am J Otol,1989,10(4):272-276.
[14]Archer CR, Cooper MH, Kveton JF. Correlation of high-resolution computed tomography and gross anatomic sections of the temporal bone:II.Vestibular apparatus
[J]. Am J Otol, 1988, 9(4):276-281.
[15]段菊如，林敏，熊俊平．面神经颞骨内段在横断薄层和CT上的定位及临床意义
[J]. 中国临床解剖学杂志, 2004, 22(3):257-260.
[16]段菊如，罗红强，鲁纯纠, 等.圆窗区区颞骨切片和CT对照观察
[J].中华耳鼻咽喉科杂志, 2004, 39(5):269-272.
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