脊髓压迫性损伤神经纤维溃变和髓鞘碱性蛋白的表达变化
伍修宇, 孙善全, 钟小燕, 张波, 汪克建, 黄思琴
中国临床解剖学杂志 ›› 2012, Vol. 30 ›› Issue (6) : 658-662.
脊髓压迫性损伤神经纤维溃变和髓鞘碱性蛋白的表达变化
Alteration of myelin basic protein expression and nerve fiber degeneration after compression injury of rat spinal cord
目的 观察脊髓压迫性损伤中白质神经纤维溃变及MBP的表达变化。 方法 采用自行设计的压迫装置制作脊髓压迫性损伤模型，运用luxol fast blue（LFB）、免疫荧光和western blot等方法来检测脊髓受压1、3、7 d后的白质纤维变化及MBP表达变化。 结果 脊髓受压后，白质髓鞘化神经纤维出现水肿，排列疏松、髓鞘缺失等退行性溃变；髓鞘化纤维数目逐渐减少。蛋白MBP表达随着压迫时间进行性下降。 结论 脊髓压迫性损伤可导致神经纤维溃变，MBP表达下调是神经纤维溃变的原因之一。
Objective To evaluate the changes of nerve fiber degeneration and myelin basic protein(MBP) expression in rat white matter after the compression spinal cord injury(CSCI). Methods Eighty-four adult Sprague-Dawley rats were randomly divided into 3 groups: normal group（n=12）, operation group（n=36） and the sham group（n=36）. The CSCI model was established with a self-made device for animals of operation group. The operation process in the sham group was the same as the operation group except for the compression of spinal cord. The segments of compressed spinal cord were properly taken at an interval of 1, 3 and 7 day after operation. Luxol fast blue(LFB)， immunofluorescence (IF) staining and western blot were performed to observe the pathological changes of white matters. Results LFB staining showed that after compression the degeneration of nerve fiber occurred in the white matter and a great number of fibers were lost. Compared with the normal and sham operation groups, the loss of myelinated fibers was markedly decreased in compressed segments of the model group (P<0.05). IF showed that MBP- positive fibers were degenerated and lost, coinciding with LFB staining. The expression of MBP was progressively significantly declined after compression. Conclusions Demyelination occurs, followed by the time-dependently reduced expression of MBP in white matter of model animals. Expression decrease of MBP may be related to the demyelination.
脊髓压迫 / 纤维溃变 / 脱髓鞘 / 髓鞘碱性蛋白 / 大鼠
Spinal cord compression / Nerve degeneration / Demyelination / Myelin basic protein / Rat
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国家自然科学基金（30270437）
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