全反式维甲酸促鼠胚神经干细胞向神经元分化中BMP-2的表达及意义

中国临床解剖学杂志 ›› 2012, Vol. 30 ›› Issue (4): 426-430.

全反式维甲酸促鼠胚神经干细胞向神经元分化中BMP-2的表达及意义

钟德君¹，李森¹，康敏²，徐双¹，魏书一¹，王清¹，王松¹

泸州医学院附属医院 1.脊柱外科， 2.内科，四川泸州 646000

收稿日期:2012-01-20 发布日期:2012-07-27
通讯作者: 康敏，E-mail:zdj_1974@163.com
作者简介:钟德君（1974-），男，四川省泸州市人，医学博士，副主任医师，硕士研究生导师，主要从事脊柱外科方面的工作
基金资助:
泸州市重点科技计划项目（2008-23-5）; 四川省卫生厅科研项目（2008-527-080178）

Expression and significance of BMP-2 during rat NSCs induced by ATRA differentiating into neurons in vitro

ZHONG De-jun¹, LI Sen¹, KANG Min², Xu Shuang¹, Wei Shu-yi¹, WANG Qing¹, WANG Song¹

1.Department of Spinal Surgery; 2.Depatment of Digestion Medicine, Affiliated Hospital of Luzhou Medical College, Luzhou 646000, China

Received:2012-01-20 Published:2012-07-27

摘要/Abstract

摘要：

目的探讨全反式维甲酸（all-trans-retinoic-acid, ATRA）体外促NSCs分化为神经元的过程中，BMP-2表达的变化及意义。方法 1.0 μmol/L ATRA诱导体外培养的鼠胚神经干细胞，诱导3、5、7、　9 d后，采用免疫细胞化学、qPCR、Western-Blot检测BMP-2表达变化。结果无ATRA干预时BMP-2表达量较高，随着诱导时间的延长，BMP-2表达逐渐降低，分化7～9 d时表达最低。结论 BMP-2在　NSCs体外分化的的早期表达较高，在NSCs分化为神经元过程中逐渐下调，表明ATRA诱导NSCs向神经元的分化与BMP-2信号的抑制有关。

关键词: 神经干细胞, 全反式维甲酸, , BMP-2, 分化, 表达

Abstract:

Objective　 To explore the expression and effect of BMP-2 during the rat embryonic stem cells(NSCs) induced by all-trans-retinoic acid（ATRA）differentiating into neurons in vitro.　Methods　 The rat embryonic neural stem cells were differentially induced with 1.0 μmol/L ATRA in vitro. The expression of BMP-2 was respectively investigated by immunocytochemistry, real-time PCR and western blot at the time of inducing 3d, 5d, 7d and 9d, and compared with that of untreated cells in control group.　Results　The highest expression of BMP-2 was at the time of inducing 3d, and then it decreased from inducing 5d following the NSCs differentiating. There was no significant difference between 7d and 9d groups.　Conclusions　The expression of BMP-2 decreases in the progress of NSCS differentiating into neurons in vitro, which indicate that, the inhibition of BMP2 signal pathway participate the differentiation process of NSCs.

Key words: Neural stem cells, All-trans-retinoic acid, BMP-2, Differentiation, Expression

中图分类号:

R338.1

钟德君, 李森, 康敏, 徐双, 魏书一, 王清, 王松. 全反式维甲酸促鼠胚神经干细胞向神经元分化中BMP-2的表达及意义[J]. 中国临床解剖学杂志, 2012, 30(4): 426-430.

ZHONG De-Jun, LI Sen, KANG Min, XU Shuang, WEI Shu-Yi, WANG Qing, WANG Song. Expression and significance of BMP-2 during rat NSCs induced by ATRA differentiating into neurons in vitro[J]. Chinese Journal Of Clinical Anatomy, 2012, 30(4): 426-430.

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