维甲酸诱导脊柱裂胎鼠脊髓组织中Caspase-3表达情况
Caspase-3 expression in spinal tissue of retinoic acid induced spina bifida fetal rat
目的 本文旨在探讨维甲酸诱导脊柱裂胎鼠脊髓组织Caspase-3表达情况。 方法 选取孕10 d Wistar大鼠，实验组用溶有维甲酸（40mg/ml）的橄榄油，以135 mg/kg经胃管注入给药制作脊柱裂畸形大鼠模型；对照组选取孕10 d Wistar大鼠给等量橄榄油。将实验组及对照组按照孕12、15、17和20 d分为4组。应用免疫组织化学方法比较分析Caspase-3在对照组、畸形组胎鼠脊髓组织细胞中的分布和表达情况。 结果 脊柱裂大鼠脊髓神经组织中Caspase-3在15 d开始增多，一直持续到20 d胚胎大鼠。其增高情况明显高于同一时间点对照组大鼠。胚胎15、17和20 d显性脊柱裂畸形鼠脊髓组织Caspase-3阳性细胞数多于对照组，荧光强度高于对照组。 结论 维甲酸诱导的脊柱裂胎鼠Caspase-3表达明显高于正常发育胎鼠。
Objective To explore caspase-3 expression in spinal tissue of retinoic acid induced spina bifida fetal rat. Methods Pregnant Wister rats with 10 days were used. Retinoic acid dissolved in olive oil (40mg /ml) were stomach fed for preparing the rat model of spina bifida malformations 135mg / kg). Control group only received olive oil. The animals were divided into 4 groups: pregnancy of 12 days, 15 days, 17 days and 20 days. Immunohistochemical method was used to detect and compare caspase-3 expression in different groups. Results The expression of caspase-3 increased at the day 15 after pregnancy, and maintained until day 20 in the spinal tissue of modeled fetal rat, which presented significant difference compared to that of control groups at the same pregnant time. At day 15, day 17 and day 20 of pregnancy, the number of caspase-3 positive cells was more in model animals than the control. Conclusions Retinoic acid induced spina bifida fetal rat demonstrates the increased caspase-3 expression in spinal tissue of fetal rats.
神经管畸形 / 脊髓组织 / 细胞凋亡 / Caspase-3
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国家自然科学基金（30872705，81070538）
京ICP备08002354号-3/
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