损伤骨骼肌组织内CD8+ T细胞的功能特性
Acute myoinjury induce the intramuscular infiltration of CD8+ CTL cells
目的 分析骨骼肌损伤后CD8+ T细胞的渗出及其功能特性。 方法 机械挤压法制备小鼠胫骨前肌（TA）损伤模型。免疫组化、免疫荧光检测损伤肌组织内CD8+ T细胞的渗出及其可能的CTL功能。 结果 HE染色证实机械挤压引发显著的TA肌纤维坏死、退变和再生。损伤肌组织内出现广泛的炎性渗出，损伤后7d（再生初期）可见较多的CD8+ T细胞，少量CD8+ T细胞共表达Perforin以及 IFN-γ。 结论 与慢性肌炎的免疫反应相似，急性损伤同样会引发CD8a+ T细胞的渗出、活化并具备 CTL功能。但这种活化的CD8+ CTL细胞仅短暂出现于肌组织的再生初期。
Objective To investigate the intramuscular infiltration of CD8+ cytotoxic T-cells （CTL cells） after crush myoinjury. Methods Tibialis antierior muscle(TA) crush was performed in C57BL/6 mice. HE staining was used to identify inflammatory reaction，degeneration and regeneration of crushed TA muscle. Immunofluorecence was used to detect pro- and anti- inflammatory cytokines, and the intramuscular infiltration of CTLs in injuried muscle. Results Crush induced significant myofiber necrosis, degeneration and regeneration. Conspicuous mononuclear cell infiltrates were detected at both d4 and d7 after crush injury. At d7 post-injury CTLs expressing perforin or IFN-γ were conspicuously detected in crushed muscle where CD8+ T-cells were found in close contact with regenerating myofibers. Conclusions After crush injury, CD8+ T cells infiltrate the injured muscle at regenerating period, and are transiently activated as CTL cells.
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