目的　观察灯盏花素对体外培养的人类创伤性瘢痕成纤维细胞生长、凋亡的影响，寻找新的治疗瘢痕的有效药物。  方法　以四甲基偶氮唑(MTT)法和流式细胞仪Annexin V/PI双染色法检测各浓度组灯盏花素对成纤维细胞增殖及凋亡的影响。  结果　各浓度组灯盏花素对体外培养的瘢痕成纤维细胞生长增殖具有明显的抑制作用(F=3.309，P<0.01)。与对照组相比，80、100、150 μmol/ml浓度组对瘢痕成纤维细胞增殖抑制具有显著差异性（P=0.015、0.006、0.000<0.05），并且呈剂量依赖性。IC50处，瘢痕成纤维细胞凋亡率与未加药组瘢痕成纤维细胞相比，差异具有显著性（P<0.05）。  结论　灯盏花素对瘢痕成纤维细胞的增殖具有显著的抑制作用，并且能够诱导其发生凋亡，为寻找有效治疗增生性瘢痕的药物 提供了新的思路。

Objective　  To study the effects of breviscapine on proliferation and apoptosis of scar-derived fibroblasts, for exploring new drugs to treat human pathological scar.　Methods　Fibroblasts were cultured as an experimental model and the effects of breviscapine were studied．Cell proliferation was determined by MTT assay. Apoptosis of cultured fibroblasts induced by breviscapine at the IC50 was detected by Annexin V/PI staining and flow-cytometry.　Results　In vitro，the proliferation of fibroblasts was significantly inhibited by breviscapine in the manner of dose-dependent(F=3.309，P<0.01). Compared with the control group, proliferation of fibroblasts was significantly inhibited in the concentration at 80,100,150 μmol/ml (P=0.015,0.006,0.000). Apoptosis rate of fibroblasts induced by breviscapine at the IC50 was significantly increased.　Conclusions　Breviscapine can significantly inhibit the proliferation and induce the apoptosis of cultured scar fibroblasts．It may be developed as a new drug for the treatment of pathological scar.