生后不同发育阶段大鼠卵巢内eNOS和iNOS的表达
屈丽华, 屈姣华, 龙治峰, 李美香, 石金凤, 罗红梅, 莫中成
中国临床解剖学杂志 ›› 2012, Vol. 30 ›› Issue (2) : 203-208.
生后不同发育阶段大鼠卵巢内eNOS和iNOS的表达
Expression of eNOS and iNOS in postnatal rat ovaries at different developmental days
目的 观察eNOS和iNOS在卵巢中的表达，并探讨其意义。 方法 采用免疫组化和图像分析系统检测大鼠生后各发育阶段卵巢中eNOS和iNOS的定位分布和表达。 结果 0 d大鼠卵巢卵母细胞eNOS和iNOS均呈弱阳性反应；4 d后，eNOS和iNOS在正常卵母细胞中均呈强阳性，退化卵母细胞中反应非常弱；卵泡细胞与膜细胞中的iNOS在30 d前均呈强阳性表达，30 d后，生长卵泡中表达降低，闭锁卵泡中较正常生长卵泡表达增强，eNOS的表达稳定；240 d 后卵巢内间质成分和闭锁卵泡增多，间质中的iNOS表达增强。360d 后大鼠卵巢逐渐纤维化，iNOS染色强于eNOS。 结论 大鼠不同发育时期卵巢中，eNOS的表达相对稳定；iNOS的表达以性成熟后育龄期最低，随着年龄增加及卵巢功能的衰退，其表达逐渐增高。
Objective To observe the expression of eNOS and iNOS in varies and investigate its significance. Methods Immunohistochemical technology and image analysis system were utilized to detect the expression of eNOS and iNOS in ovaries from postnatal rat at different developmental days. Results Positive reaction for eNOS and iNOS was weak in oocytes of rat ovaries at day 0. In ovaries from older rat than day 4, both of eNOS and iNOS immunoreactivity were intense in health oocytes and very weak in degenerative oocytes. Expression of iNOS was strong positive in follicular cells and theca cells of ovaries from younger rat than day 30，but became very weak in those of growing follicles from older rat than day 30, and increased in those of atretic follicles compared with those in health growing follicles. Positive expression of eNOS was constant. Interstitium and atretic follicles increased in ovaries from older rat than day 240, and interstitium showed an enhanced expression for iNOS. Ovaries from older rat than day 360 became fibrosis, in which iNOS stain was more intensive than eNOS. Conclusion In rat ovaries from different development stages, expression of eNOS is relatively stable and expression of iNOS is the minimum in child-bearing period after sex maturation, but increases gradually with rat age (month) progressing and ovarian functional deterioration.
卵巢 / eNOS / iNOS / 免疫组织化学 / 大鼠
Ovary / eNOS / iNOS / Immunohistochemistry / Rat
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