离子硅对成骨细胞NF-kappa B核内转录因子活性的影响
邱小忠, 王永魁, 王乐禹, 余磊, 王国保
中国临床解剖学杂志 ›› 2012, Vol. 30 ›› Issue (1) : 84-86.
离子硅对成骨细胞NF-kappa B核内转录因子活性的影响
Effects of silicon on the activity of the nuclear transcription factor NF-kappa B in osteoblasts
目的 研究离子硅对体外培养的成骨细胞NF-kappa B核转录因子活性的影响。 方法 分别用终浓度为1 mmol/L和2 mmol/L离子硅（SiO32-）处理MC3T3-E1成骨细胞，处理时间分别为6、12、24和48 h，设置对照组（不加处理因素）；采用流式细胞术检测细胞周期,计算细胞的增殖指数；Western blotting方法检测NF-kappa B信号通路的相关蛋白表达量及其变化。 结果 流式细胞术结果显示，与对照组相比，1 mmol/L浓度的离子硅处理24 h组和48 h组，MC3T3-E1细胞增殖明显；Western blot结果显示，1 mmol/L浓度的离子硅促进成骨细胞增殖与p-NF-kappa B表达上升密切相关。 结论 骨材料中释放的微量的硅不会引起成骨细胞损伤，相反，微量的硅酸盐可能通过激活NF-kappa B诱导成骨细胞增殖。
Objective To explore the effects of silicon on the activity of nuclear transcription factor NF-kappa B in osteoblasts cultured in vitro. Methods MC3T3-E1 osteoablasts were processed by sodium silicate solution (SiO32-) with the final concentration of 1mM and 2 mM for 6h, 12h, 24h and 48h, respectively. Untreated cells were taken as the control groups. Flow cytometry was used to detect the cell cycle and calculate cell proliferation index. Western blotting was used to detect the expressions and changes of NF-kappa B signaling pathway related proteins. Results Compared with the controls, significant proliferation of MC3T3-E1 cells was found in the 24h and 48h groups treated with 1 mM silicon. Western blot analysis showed that the pro-proliferation effect of 1 mM silicon on osteoblasts was closely related to the high-expression of p-NF-kappa B. Conclusions Trace amounts of silicon released from bone materials can not cause damage of osteoblasts, on contrary, trace amounts of silicate may induce proliferation of osteoblasts by activating NF-kappa B signaling pathway.
硅 / 生物材料 / 骨修复 / 成骨细胞 / NF-kappa B
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