低频电磁场对BDNF基因修饰的BMSCs增殖影响的实验研究
邱家琦, 张鸣生, 李新平, 白文芳, 吴博, 白利明, 徐武华
中国临床解剖学杂志 ›› 2012, Vol. 30 ›› Issue (1) : 74-78.
低频电磁场对BDNF基因修饰的BMSCs增殖影响的实验研究
The effects of low frequency electromagnetic fields on the proliferation of BMSCs modified by BDNF gene
目的 探讨低频电磁场（LFEMF）对脑源性神经营养因子（BDNF）基因修饰的骨髓间充质干细胞（BMSCs）增殖的影响。 方法 通过同源重组的方法，用pAd-easy I腺病毒包装系统在体外构建含BDNF基因的重组腺病毒表达载体(rADBDNF)；体外分离纯化SD大鼠来源的BMSCs，rADBDNF感染后，用LFEMF（50 Hz、5 mT）进行干预，连续干预3 d，并设对照组、BDNF基因修饰组、LFEMF组。在倒置相差显微镜下，对经台盼蓝染色的各组细胞进行计数；四甲基偶氮唑蓝（MTT）法对各组细胞的增殖效率进行检测；流式细胞仪分析细胞周期变化。 结果 LFEMF作用于BDNF基因修饰后的BMSCs，细胞增殖速率与对照组、BDNF基因修饰组比较，差异有统计学意义（P<0.05），与LFEMF组相比，差异无统计学意义（P>0.05），G2/M期细胞数量明显增多。 结论 LFEMF干预可促进BDNF基因修饰后BMSCs增殖。
Objective To explore the effects of low frequency electromagnetic fields on the proliferation of BMSCs modified by Brain-derived neurotrophic factor(BDNF) gene. Methods The replication-deficient recombinant adenoviruses containing BDNF gene（rADBDNF）was structured by the pAd-easy I adenovirus packaging systems in vitro. Rat BMSCs were isolated from rat femoral and tibial bone marrow, cultured and identified. BMSCs infected with rADBDNF were exposed to low frequency electromagnetic fields. The proliferation capacity of BMSCs was analyzed using the trypan blue staining assay and MTT assay, and cell cycles of BMSCs was evaluated by flow cytometry. Results BMSCs modified by rADBDNF had no significant change on cell proliferation compared with that of untreated cells (P>0.05). However, electromagnetic field exposed MSCs(LFEMF group) had a significant proliferative improvement compared with the control(P<0.05). The ratio of G2/M cell of the transgenic and LFEMF group was also increased. Conclusions LFEMF could enhance the cell proliferation of rat BMSCs modified by BDNF gene.
骨髓间充质干细胞 / 细胞增殖 / 脑源性神经营养因子 / 低频电磁场
Bone mesenchymal stem cells(BMSCs) / Cell proliferation / Brain-derived neurotrophic factor(BDNF) / Low frequency electromagnetic fields(LFEMF)
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