神经分泌素对体外培养血管平滑肌细胞VCAM、MCP-1和Ki67表达的影响
伍校琼, 罗明英, 朱武, 杨宝林, 罗华, 蔡维君
中国临床解剖学杂志 ›› 2012, Vol. 30 ›› Issue (1) : 70-73.
神经分泌素对体外培养血管平滑肌细胞VCAM、MCP-1和Ki67表达的影响
Effects of secretoneurin on VCAM, MCP-1 and Ki67 expression in blood vessel smooth muscle cell
目的 观察神经分泌素(Secretoneurin，SN)对体外培养血管平滑肌细胞（vascular smooth muscle cells，VSMC）粘附分子（VCAM）、单核细胞趋化因子（MCP-1）和Ki67（细胞增殖标记物）表达的影响。 方法 将体外培养的成年大鼠胸主动脉血管平滑肌细胞随机分成正常对照组和SN实验组，应用免疫荧光细胞化学技术检测VCAM、MCP-1和Ki67的表达，Nikon共聚焦显微镜观察并拍照，图片用EZ-C1 3.70 FreeViewer进行处理。 结果 免疫荧光显示，正常对照组VCAM和MCP-1表达微弱，Ki67阳性细胞很少；SN作用24 h后，VCAM、MCP-1的表达和Ki67的阳性细胞数目明显增多。其中VCAM-1、MCP-1的免疫细胞荧光强度和Ki67的阳性细胞率在两组间差异有显著性 (P＜0.05)。 结论 SN可上调粘附分子和单核趋化因子的表达，同时促进血管平滑肌细胞的增殖。
Objective To investigate the effect of secretoneurin on expression of VCAM, MCP-1 and Ki67 in blood vessel smooth muscle cells (VSMCs). Methods VSMCs culture from adult mouse thoracic aorta were randomly divided into normal control and SN experiment. The expression of VCAM, MCP-1 and Ki67 were detected by immunofluorescene. The result was viewed and recorded under confocal instrument，and the data were processed with EZ-C1 3.70 FreeViewer system．Results The expression of VCAM, MCP-1 and Ki67 in VSMCs treated with SN about 24h were significantlty increased compared to that of normal control (P＜0.05). Conclusions SN up-regulate the expression of VCAM, MCP-1，and promote the proliferation of vascular smooth muscle cells．
神经分泌素 / 平滑肌细胞 / 增殖 / 粘附分子 / 单核细胞趋化因子
Secretoneurin / Vascular smooth muscle cells / Proliferation / Vascular cell adhesion molecule / Monocyte chemoattractant protein
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国家自然科学基金（30771134）（30971532）; 博士点课题（20090162110063）
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