复方丹参对严重烫伤大鼠血脑屏障的保护作用

中国临床解剖学杂志 ›› 2011, Vol. 29 ›› Issue (5): 565-567.

复方丹参对严重烫伤大鼠血脑屏障的保护作用

刘曾旭¹，　余　庆²，　王向东³，　周聪发¹，　李耀斌¹，　刘德明¹

南昌大学 1.基础医学院解剖学教研室, 2. 图书馆，南昌 330006； 3. 江西护理职业技术学院解剖学教研室，南昌 330201

收稿日期:2011-01-06 出版日期:2011-09-25 发布日期:2011-09-29
通讯作者: 刘德明，博士生导师，Tel:(0791)8614152，E-mail:anajx@　sina.com E-mail:lzx6698@yahoo.com.cn
作者简介:刘曾旭（1967-），男，江西省宁都县人，博士，教授，硕士生导师，主要研究方向：神经损伤与再生，Tel:(0791)8160597
基金资助:
江西省科技支撑计划（2009BSB09500），江西省卫生厅课题（20062028）

The protection of salvia miltiorrhiza compostita to blood brain barrier on severe burn injuried rats

LIU Zeng-xu¹, YU Qing² , WANG Xiang-dong³, ZHOU Chong-fa¹, LI Yao-bin¹, LIU De-ming¹

1. Department of Anatomy, Basic Medical College of Nanchang University ,Nanchang, 330036, China；2. Library of Nanchang University, Nanchang, 330036, China；3. Department of Anatomy, Jiangxi Nursing College, Nanchang 330201, China

Received:2011-01-06 Online:2011-09-25 Published:2011-09-29

摘要/Abstract

摘要：

目的　观察复方丹参注射液对严重烫伤大鼠血脑屏障通透性及脑内ZO-1mRNA影响，探讨复方丹参对严重烫伤大鼠血脑屏障损伤的保护作用。方法　将大鼠随机分成假烫伤组、烫伤组、复方丹参治疗组。紫外分光光度计检测各组大鼠脑组织内伊文氏蓝的含量，RT-PCR检测严重烫伤大鼠脑内ZO-1 mRNA表达变化。结果　大鼠严重烫伤后3～48 h脑内ZO-1mRNA表达下降。脑内伊文氏蓝（EB）含量从烫伤后3h开始升高，在6～12 h显著升高。用复方丹参治疗后, 严重烫伤大鼠脑内EB含量降低，以3、6 h下降最明显（P<0.010）；脑内ZO-1mRNA表达升高，以烫伤3 h治疗组最为明显。结论　复方丹参注射液可防止严重烫伤大鼠脑内ZO-1mRNA的下降，减轻严重烫伤对大鼠血脑屏障的损害。

关键词: 烫伤, 血脑屏障, 紧密连接, 复方丹参

Abstract:

Objective　To explore the effects of salvia miltiorrhiza compositita on blood-brain barrier permeability and ZO-1 expression in burn injuried rats.　Methods　In this research, healthy Sprague-Dawley rats were separated into three groups as follows: sham scald group, scald group, scald and salvia miltiorrhiza group. Changes in BBB permeability were determined by detected Evans blue (EB) content in rats' brain with chemical method. Furthermore, in order to explore the molecular mechanism of BBB disruption in rats' brain after severe burn, the expression levels of ZO-1 mRNA was analyzed by semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR).　Results　Expression of brain ZO-1mRNA significantly decreased during 3 to 48h after burn. The contents of Evan's bluedye (EB) in brain significantly improved at the 3h and reached the highest level during 6 to 24h after burn injury. After treating with salvia miltiorrhiza, EB content in the brain of burned rats was corrected obviously, especially at the time points of 3, 6h after burn injury. As well, ZO-1mRNA in brain increased after drug treating, and reached the highest level at 3h after burn injury.　Conclusions　Salvia miltiorrhiza will prevent the decrease of ZO-1 level in brain after burn injury, which is helpful for alleviating the burn damage on blood-brain barrier.

Key words: Burn injury, Blood-brain barrier, Zonula occludens, Salvia miltiorrhiza compostita(SMC)

中图分类号:

R644

刘曾旭, 余庆, 王向东, 周聪发, 李耀斌, 刘德明. 复方丹参对严重烫伤大鼠血脑屏障的保护作用[J]. 中国临床解剖学杂志, 2011, 29(5): 565-567.

LIU Ceng-Xu, TU Qiang, WANG Xiang-Dong, ZHOU Cong-Fa, LI Yao-Bin, LIU De-Meng. The protection of salvia miltiorrhiza compostita to blood brain barrier on severe burn injuried rats[J]. Chinese Journal Of Clinical Anatomy, 2011, 29(5): 565-567.

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