Toll样受体2和4在髋关节滑膜巨噬细胞中的表达

中国临床解剖学杂志 ›› 2011, Vol. 29 ›› Issue (5): 557-560.

Toll样受体2和4在髋关节滑膜巨噬细胞中的表达

曾展鹏¹,　丁　悦¹,　许　杰¹,　黄健斌¹,　秦础强¹,　文淑萍¹,　Barden Bertram²

1.中山大学附属孙逸仙纪念医院骨外科，广州 510120；　2.德国亚琛大学附属Düren医院骨外科，德国 45147

收稿日期:2011-04-15 出版日期:2011-09-25 发布日期:2011-09-29
通讯作者: 丁　悦，主任医师，硕士生导师，E-mail: dingyue36@　hotmail.com E-mail:baileysea@163.com
作者简介:曾展鹏（1985-），男，广州人，硕士研究生，学士，研究方向：骨科，关节外科
基金资助:
国家自然科学基金(81071489)；广东省科技计划社会发展基金(2009B030801140)；广东省自然科学基金(8151008901000034)

The expression of Toll-like receptors 2 and 4 in the macrophages of hip synovium

ZENG Zhan-peng¹, DING Yue¹, XU Jie¹, HUANG Jianbin¹, QIN Chu-qiang¹, WEN Shu-ping¹, Barden Bertram²

1.Department of Orthopedic Surgery，Sun Yat-sen Memorial Hospital，Guangzhou , 510120，China; 2.　Department of Orthopedic Surgery of Düren Hospital of the RWJH Achen University, Germany，45147

Received:2011-04-15 Online:2011-09-25 Published:2011-09-29

摘要/Abstract

摘要：

目的　探讨TLR2和TLR4在髋关节滑膜巨噬细胞中的表达。方法　收集2007年至　2010年因髋关节疾病在我院行髋关节手术患者的髋关节滑膜标本共47例，其中股骨颈骨折24例（A组），股骨头坏死18例（B组），人工髋关节置换术后假体无菌性松动5例（C组）。采用免疫组化SP法检测TLR2和TLR4在3组的髋关节滑膜巨噬细胞中的表达,在高倍镜（×400）视野下对阳性的巨噬细胞的进行观察及计数，并作统计分析。结果　各组用histoscore计算阳性巨噬细胞百分数得分，每高倍视野下A组中TLR2（0.27±0.33），TLR4 （0.69±0.18）；B组中TLR2 （0.31±0.19），TLR4 （0.71±0.31）；C组中　TLR2 （1.78±0.18），TLR4 （2.00±0.39）。TLR2和TLR4在C组中表达较A组、B组高（P＜0.001），而且3组中TLR4均较TLR2表达要高（P＜0.001）。结论　TLR2及TLR4在人工关节无菌性松动患者的假体周围组织巨噬细胞中表达明显增多，可能参与了巨噬细胞介导的假体无菌性松动过程。

关键词: , Toll样受体, 髋关节, 滑膜, 巨噬细胞, 免疫组化技术

Abstract:

Objective　To explore the expression of TLR2 and TLR4 in the macrophages of hip synovium. Methods　Hip synovium tissues were collected from 47 cases during hip surgery in our hospital from 2007 to 2010 and divided into three groups. 24 cases of femoral neck fracture were in group A, 18 cases of femoral head necrosis were in group B, and 5 cases of aseptic loosening were in group C. TLR2 and TLR4 were detected in synovial tissues of three groups by immunohistochemistry (sp), and the positive macrophages were observed, counted and analyzed statistically. Results　The histoscore of TLR2 was 0.27±0.33/HP in Group A, 0.31±0.19/HP in Group B and 1.78±0.18/HP in the Group C. The histoscore of TLR4 was 0.69±0.18/HP in Group A, 0.71±0.31/HP in Group B and 2.00±0.39/HP in Group C. The expression of TLR2 and TLR4 in group C was significantly higher than that in group A and group B(P＜0.001). The expression of TLR4 was significantly higher than TLR2 among the three groups(P＜0.001). Conclusions　TLR2 and TLR4 significantly increases in the macrophages of hip synovium around the prosthesis, which may be involved in macrophage-mediated process of aseptic joint loosening.

Key words: Toll-like receptors (TLRs), Hip joint, synovium, Macrophages, Immunohistochemisty

中图分类号:

R687.4

曾展鹏, 丁悦, 许杰, 黄健斌, 秦础强, 文淑萍, Barden Bertram. Toll样受体2和4在髋关节滑膜巨噬细胞中的表达[J]. 中国临床解剖学杂志, 2011, 29(5): 557-560.

CENG Zhan-Feng, DING Yue, HU Jie, HUANG Jian-Bin, QIN Chu-Jiang, WEN Chu-Ping, Barden Bertram. The expression of Toll-like receptors 2 and 4 in the macrophages of hip synovium[J]. Chinese Journal Of Clinical Anatomy, 2011, 29(5): 557-560.

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