关键词: 大鼠, 全身炎症反应综合征, 动物模型

Abstract:

Objective　To establish a reproducible experimental rat model of traumatic systemic inflammatory response syndrome.　Methods　Thirty healthy Wistar rats were randomly assigned to the control group (group A), the LPS injury group (group B) and the surgical treatment group (group C). For preparing trauma model, the rats of group B were performed intravenous infusion of lipopolysaccharide (LPS, 12mg/kg), and the rats of group C were cut the liver (1/3) and hammered right femur three times. 2 hours later after the treatment, the alanine aminot ransferase (ALT), aspartete aminot ransferase (AST), urea nit rogen (UN) and creatinine (Cr) in serum were surveyed. At the 12 hours, the rats were killed and the liver and lung were taken for HE staining and comparision.　Results　The concentration of serous ALT, AST, UN and Cr in group B and C were significantly higher than that of group A ( P<0.01). Compared to that of group B, Serous UN and Cr in group C were significantly higher (P<0.05). For inflammatory reaction and infiltration in liver and lung, there was no significant difference between group B and C.　Conclusions　The rat model of traumatic systemic inflammatory response syndrome can be established successfully using this surgical process.

Key words: Rat, systemic inflammatory response syndrome, SIRS, Animal model