1型糖尿病大鼠左心室HCN2和HCN4重构
The expression of HCN2 and HCN4 in the left ventricle of type 1 diabetes rat
目的 探讨1型糖尿病大鼠左心室肌HCN2和HCN4的表达时空变化。 方法 60只大鼠随机分为正常对照组(N组, n=20)和1型糖尿病组(T1DM组, n=40)：腹腔注射链脲佐菌素制作大鼠1型糖尿病模型。90 d后，取左心室肌通过免疫荧光法检测HCN2、HCN4的定位；Western-blot、RT-PCR技术检测HCN2、HCN4在蛋白质及mRNA水平的变化。 结果 免疫荧光结果显示HCN2在心室肌细胞膜上呈点状或短线状不连续表达；HCN4未见表达。Western-blot、RT-PCR结果显示T1DM组大鼠 HCN2、HCN4在蛋白及mRNA水平表达升高。 结论 1型糖尿病大鼠左心室出现离子通道HCN2、HCN4表达升高，导致糖尿病心脏电重构，可能与糖尿病引起的室性心律失常有关。
Objective To investigate the expression of HCN2 and HCN4 in the left ventricles of type 1 diabetes rat. Methods 60 rats were randomly divided into control group (N, n=20) and diabetes group (T1DM, n=40). For T1DM group, streptozotocin was injected into peritoneal cavity of the animals to establish the model of type 1 diabetes. At the 90d after injecting, the expression of HCN2 and HCN4 were detected by immunofluorescent staining, Western-blot and RT-PCR respectively. Results Immuno-fluorescent staining presented the discontinuous punctiform or linear expression of HCN2 in cardiomyocytes membrane, but not for HCN4. However, Both HCN2 and HCN4 were up-regulated at protein(western-blot) and RNA (RT-PCR) levels compared to that of controls. Conclusions The over expression of HCN2 and HCN4 maybe participate in the electrical remodeling in DM rats and the developing of ventricular arrhythmia.
HCN2 / HCN4 / 左心室 / 1型糖尿病 / 大鼠
HCN2 / HCN4 / Left ventricle / T1DM / Rat
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河南省杰出青年科学基金（2005HANCET-15）
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