目的　探讨眉间纹形成的解剖学机制，为肉毒素注射除皱提供形态学基础。  方法　对6例成人头部标本的降眉肌、降眉间肌、皱眉肌进行解剖观测、标志点定位、肌肉称重及肌肉横截面积估算。  结果　眉间区域表情肌起点较恒定，止点为一个部位，各表情肌之间联系紧密，可称为“联合体”，其中以“降眉肌-降眉间肌”联合体最明显。眉间动力性皱纹主要由皱眉肌和降眉肌收缩时产生的水平方向力对眉间区域皮肤的协同作用所致。皱眉肌和降眉肌生理横截面积之比=6：1。皱眉肌：降眉肌水平分解力比值约为（8~10）:1。  结论　皱眉肌和降眉肌为肉毒素注射治疗眉间纹的目标肌肉，注射部位应选在皱眉肌起端1/3处和降眉肌与降眉间肌结合部位。

Objective　To investigate the anatomic formation of the glabella muscles and provide anatomical basis for the use of Botulinum Toxin Injection.  Methods　6 adult cephalic specimens were anatomized. The landmarks of the glabella muscles, including the corrugator, the depressor supercilii and the procerus were measured. The weights and cross-sectional area of the muscle were also examined.　Results　The origin of the glabella muscles is constant, while the insertion is an area which is hard to define. Moreover, each pathetic muscle is connected closely to form a "combination complex", especially between the depressor supercilii and the procerus. The appearance of the glabellar wrinkle is formed by horizontal force generated through contraction of the corrugators and the depressor supercilii. The proportion of the physiological cross-sectional area is 6:1 between the corrugator and the depressor supercilii. The proportion of the clinical anatomy horizontal force is（8-10）:1 between the corrugator and the depressor supercilii.　Conclusions　The corrugators and the depressor supercilii are the potent targets for Botulinum Toxin Injection. Injection points was selected at originating 1/3 of the corrugator point and depressor supercilii combining parts .