骨髓间充质干细胞与纳米羟基磷灰石支架材料的体外相容性研究
In vitro biocompatibility of bone marrow mesenchymal stem cells and nano-hydroxyapatite scaffold
目的 探讨兔骨髓间充质干细胞(rBMSCs)与纳米羟基磷灰石(nano-HA)支架材料的相容性，进一步验证nano-HA材料作为骨组织工程支架材料的可行性。 方法 将rBMSCs与nano-HA支架材料在体外复合培养，通过倒置显微镜、扫描电镜观察细胞与材料的复合情况，用MTT法、碱性磷酸酶活性检测法检测材料对细胞增殖、分化的影响。 结果 rBMSCs可以在nano-HA支架材料表面及孔隙中良好的黏附、迁移、增殖和分化，复合培养5 d后nano-HA支架材料对rBMSCs的增殖分化表现出一定的促进作用。 结论 rBMSCs与nano-HA支架材料具有良好的生物相容性, nano-HA支架材料可以作为rBMSCs良好的载体。
Objective To assess the compatibility of rabbit bone marrow mesenchymal stem cells(rBMSCs) with nano-hydroxyapatite(nano-HA) scaffold，and to verify the feasibility of nano-HA material as scaffold material of bone tissue engineering. Methods rBMSCs were cultured on nano-HA scaffold in vitro. Inverted microscope and scanning electron microscope were used to observe the morphologic features of cultured cells attached on the scaffold, as well, cell proliferation and differentiation under the influence of the material were detected by MTT and ALP methods. Results On the nano-HA scaffold, rBMSCs attached and survived well. Scaffold did not interfere with the proliferation and differentiation of the seeded rBMSCs, which indicated that Nano-HA scaffold was favourable for the survival and differentiation of rBMSCs in vitro condition. Conclusions Nano-HA material can be taken as the ideal rBMSCs scaffold for its excellent biocompatibility.
Bone marrow mesenchymal stem cells(rBMSCs) / Nano-hydroxyapatite / Biocompatibility
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