目的　构建hPDGE-BB(human platelet-derived growth factor-BB)腺病毒表达载体并观察其在人表皮干细胞(human epidermal stem cells, hESCs)中PDGF-BB蛋白的表达情况。   方法　从质粒pCMV-SPORT6上通过PCR扩增hPDGF-BB基因，将其酶切后连接到穿梭质粒pAd-track-CMV上，线性化后在BJ5183细菌中和骨架质粒pAdeasy-1进行同源重组，筛选阳性克隆并酶切鉴定，线性化后转染入HEK293细胞中进行包装、扩增，得到重组腺病毒rAD-PDGF。原代培养hESCs，流式细胞术分析hESCs的纯度，用收集的腺病毒感染靶细胞hESCs后，Westorn blot检测其表达PDGF-BB情况。　结果　成功使重组腺病毒载体rAD-PDGF携带PDGF-BB基因，流式细胞术检测hESCs的纯度达88%以上，　rAD-PDGF成功导入hESCs后，Westorn blot检测hESCs能表达PDGF-BB蛋白。　结论　成功构建的重组腺病毒rAD-PDGF在hESCs中表达PDGF-BB蛋白。

Objective　To construct the recombinant adenovirus vector containing PDGF-BB gene and detect its expression in human epidermal stem cells (hESCs).　Methods　The core sequence of human PDGF-BB was amplified by PCR from pCMV-SPORT6, and then was cloned to pAd track-CMV. The linearized shuttle plasmid was homogenously recombined with pAdeasy-l in BJ5183, and the potential clone was analyzed by restriction endonuclease digestion. The correct clone was linearized and transfected into HEK293 cells for packing and amplifying so as to obtain adenovirus rAD-PDGF. hESCs were separated from the human prepuce, cultured in vitro, and identified by flow cytometry. hESCs were infected by the harvested virus, and the protein expression of PDGF-BB in hESCs was respectively detected by Western blot.　Results　PDGF-BB was correctly cloned into the adenovirus vector, and the percentage of putative epidermal stem cells showed expression of the molecular marker was about 88%. The recombinant adenovirus vector containing human PDGF-BB gene was constructed successfully, which could infect hESCs efficiently, with the expression of the PDGF-BB in the infected hESCs detected by Western blotting.　Conclusions　 The human ESCs infected by constructed adenovirus vector rAD-PDGF could express PDGF-BB successfully.