应力刺激下兔冈上肌腱急性断裂术后腱-骨修复Ⅰ、Ⅲ型胶原的表达变化

中国临床解剖学杂志 ›› 2010, Vol. 28 ›› Issue (3): 308-.

应力刺激下兔冈上肌腱急性断裂术后腱-骨修复Ⅰ、Ⅲ型胶原的表达变化

李　森，　靳安民，　付国建，　王鹏成，　闵少雄，　张　辉

南方医科大学附属珠江医院骨科中心，广州 510282

收稿日期:2009-12-30 出版日期:2010-05-25 发布日期:2010-06-11
通讯作者: 靳安民，教授，主任医师，博士后导师，Tel：13802983267，E－mail:jinanmin2008@163.com E-mail:jht187@163.com
作者简介:李森（1979-），男，在读博士，主治医师，主要从事脊柱、脊髓损伤及骨关节损伤方面研究

The effects of stress on Ⅰand Ⅲ collagen expression in the repaired conjunction between tendon and bone after acute rupture of rabbit supraspinatus tendon

LI Sen, JIN An-min, FU Guo-jian, et al.

Department of Orthopaedics, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China

Received:2009-12-30 Online:2010-05-25 Published:2010-06-11

摘要/Abstract

摘要：

目的　研究应力刺激对其腱-骨修复Ⅰ、Ⅲ型胶原蛋白的表达影响，探讨应力刺激在肩袖损伤术后腱-骨修复中的作用。方法　选取18只成年雄性新西兰白兔，术前随机选取两只白兔处死，以熟悉解剖结构及对照用。余16只白兔随机分为2组: A组（应力刺激组）与B组（非应力刺激组）。A组术后第2周开始训练;B组正常笼养。分别于术后2、4、6、8周训练结束后处死，取材,每次每组各处死两只，行Ⅰ、Ⅲ型胶原蛋白的表达检测。结果　⑴术后2周，两组胶原染色阳性，但无明显差别；⑵术后4周，两组胶原染色阳性，A组Ⅰ、Ⅲ型胶原染色颜色均较B组深，且稍显均匀，犹以Ⅲ型胶原为明显；⑶术后6周，Ⅰ、Ⅲ型胶原染色阳性分布均较前广泛, A组较B组明显，且胶原纤维排列相对有序;⑷术后8周,A组胶原排列相对更为有序,Ⅰ型胶原染色较Ⅲ型更为均匀分布,Ⅲ型胶原明显减少。结论　应力刺激能够促进兔冈上肌腱急性断裂术后腱-骨界面胶原合成，从而促进肩袖损伤术后腱-骨修复。

关键词: 兔冈上肌腱!腱-骨修复!应力刺激!Ⅰ、Ⅲ型胶原

Abstract:

Objective　To explore the effects of stress on Ⅰ and Ⅲ collagen expression in the repaired conjunction between tendon and bone after acute rupture of rabbit supraspinatus tendon. Methods　18 adult male New Zealand white rabbits were selected in this study. Two of them were taken as the controls randomly. Another 16 animals were randomly divided into two groups: stress stimulation group (group A, n=8) and no stimulation group (group B, n=8). 2 weeks after operation of tendon rupture, animals of group A were trained, while of group B were normally feed. After 2, 4, 6 and 8 weeks ,respectively, 2 rabbits at each time in each group were put to death, and prepared the specimens for detecting the expression of Ⅰ and Ⅲ collagens. Results　Collagen fibers appeared in repaired tissue for each group at the second week of the operation, with no significant difference; however, collagen expression was more strong in group A than that in group B, especially for Ⅲ collagen at the 4 weeks after operation; at the 6 weeks after operation, Ⅰ and Ⅲ collagens stained more strong and wide, followed by orderly arranged collagen fibers, compared with that of previous time points, furthermore, well-arranged and stained uniformly Ⅰ type collagen fibers appeared in A group at the 8 week. Conclusions　Stress stimulation is favorable for promoting collagen synthesis of the repaired tendon-bone interface, which will contribute to the recovery of damaged rotator cuff.

Key words: Rabbit supraspinatus tendon!Tendon-bone repair!Stress stimulation!Ⅰand Ⅲ collagen

中图分类号:

R686.5

李　森, 靳安民, 付国建, 王鹏成, 闵少雄, 张　辉. 应力刺激下兔冈上肌腱急性断裂术后腱-骨修复Ⅰ、Ⅲ型胶原的表达变化[J]. 中国临床解剖学杂志, 2010, 28(3): 308-.

LI 　Sen, JIN An-Min, FU Guo-Jian, WANG Feng-Cheng, MIN Shao-Xiong, ZHANG 　Hui. The effects of stress on Ⅰand Ⅲ collagen expression in the repaired conjunction between tendon and bone after acute rupture of rabbit supraspinatus tendon[J]. Chinese Journal Of Clinical Anatomy, 2010, 28(3): 308-.

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