GATA2促进FAK/STAT3信号通路活化增强宫颈癌细胞恶性行为

GATA2促进FAK/STAT3信号通路活化增强宫颈癌细胞恶性行为

陈羡盈, 王若涵, 夏瑜, 王永军

中国临床解剖学杂志 ›› 2026, Vol. 44 ›› Issue (5) : 559-564.

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中国临床解剖学杂志 ›› 2026, Vol. 44 ›› Issue (5) : 559-564. DOI: 10.13418/j.issn.1001-165x.2026.5.09

GATA2促进FAK/STAT3信号通路活化增强宫颈癌细胞恶性行为

陈羡盈^{1, 2}，王若涵²，夏瑜²，王永军^{1, 2}*

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GATA2 enhances malignant biological behaviors of cervical cancer cells by promoting activation of FAK/STAT3 signaling pathway

Chen Xianying^1,2, Wang Ruohan ², Xia Yu², Wang Yongjun^1,2*

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摘要

目的探讨GATA2对宫颈癌细胞恶性生物学行为及FAK/STAT3信号通路活化的影响。方法 qRT-PCR及Western blot技术检测GATA2在宫颈癌细胞系（HeLa、CaSki）和正常宫颈上皮细胞HUCEC中的表达。将靶向GATA2的小干扰RNA（si-GATA2）及其阴性对照（si-NC）转染至HeLa和CaSki细胞以敲减GATA2， qRT-PCR和Western blot验证敲减效率。 Western blot检测敲减GATA2对FAK/STAT3通路相关蛋白（p-FAK、FAK、p-STAT3、STAT3）表达的影响。HeLa和CaSki细胞分为si-NC（对照组）、si-GATA2组和si-GATA2+ZINC40099027（FAK激活剂）组，细胞集落形成实验、流式细胞术和Transwell实验分别检测细胞增殖、凋亡、迁移及侵袭能力。结果与HUCEC细胞相比，GATA2在HeLa和CaSki细胞中的mRNA和蛋白表达水平均显著升高。转染si-GATA2后，HeLa和CaSki细胞中GATA2的mRNA和蛋白表达水平均显著降低。敲减GATA2可显著降低p-FAK和p-STAT3蛋白水平，而对FAK和STAT3总蛋白表达无显著影响。功能实验显示，敲减GATA2显著抑制HeLa和CaSki细胞集落形成能力、诱导细胞凋亡、抑制细胞迁移和侵袭能力。但加入FAK激活剂ZINC40099027后，敲减GATA2所介导的上述抑癌效应被部分逆转。结论宫颈癌细胞中GATA2表达水平显著升高，并可能通过促进FAK/STAT3信号通路活化，增强肿瘤细胞的增殖、迁移和侵袭，同时抑制细胞凋亡。

Abstract

Objective To investigate the effects of GATA2 on the malignant behaviors of cervical cancer cells and activation of the FAK/STAT3 signaling pathway. Methods GATA2 expression in cervical cancer cell lines (HeLa, CaSki) and normal cervical epithelial HUCEC cells was examined by qRT-PCR and Western blot, respectively. Small interfering RNA targeting GATA2 (si-GATA2) and its negative control (si-NC) were transfected into HeLa and CaSki cells to knock down GATA2, and the knockdown efficiency was verified by qRT-PCR and Western blot. The effects of GATA2 knockdown on the expression of FAK/STAT3 pathway-related proteins (p-FAK, FAK, p-STAT3, STAT3) were assessed by Western blot. HeLa and CaSki cells were divided into 3 groups: si-NC (control), si-GATA2, and si-GATA2+ZINC40099027 (FAK activator). Colony formation assay was used to evaluate cell proliferation, flow cytometry to detect apoptosis rate, and Transwell assay to measure cell migration and invasion. Results Compared with HUCEC cells, the mRNA and protein expression levels of GATA2 were significantly elevated in HeLa and CaSki cells. Following transfection with si-GATA2, GATA2 mRNA and protein levels were markedly reduced in both HeLa and CaSki cells. Knockdown of GATA2 significantly decreased the expression levels of p-FAK and p-STAT3 proteins, but had no significant effect on total FAK and STAT3 protein levels. Functional assays revealed that GATA2 knockdown significantly inhibited colony formation, induced apoptosis, and suppressed the migration and invasion of HeLa and CaSki cells. However, these tumor-suppressive effects mediated by GATA2 knockdown were partially reversed upon the addition of the FAK activator ZINC40099027. Conclusions GATA2 expression is significantly elevated in cervical cancer cells and may enhance tumor cell proliferation, migration and invasion while inhibiting apoptosis by promoting activation of the FAK/STAT3 signaling pathway.

关键词

GATA2 / / / 宫颈癌 / / / FAK/STAT3信号通路 / / / 迁移 / / / 侵袭

Key words

GATA2 / / / Cervical cancer / / / FAK/STAT3 signaling pathway / / / Migration / / / Invasion

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陈羡盈, 王若涵, 夏瑜, 王永军. GATA2促进FAK/STAT3信号通路活化增强宫颈癌细胞恶性行为[J]. 中国临床解剖学杂志. 2026, 44(5): 559-564 https://doi.org/10.13418/j.issn.1001-165x.2026.5.09

Chen Xianying, Wang Ruohan, Xia Yu, Wang Yongjun. GATA2 enhances malignant biological behaviors of cervical cancer cells by promoting activation of FAK/STAT3 signaling pathway[J]. Chinese Journal of Clinical Anatomy. 2026, 44(5): 559-564 https://doi.org/10.13418/j.issn.1001-165x.2026.5.09

中图分类号： R322.6 R737.33

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基金

北京积水潭医院-自然基金培育计划项目（ZR-202215）；首都卫生发展科研专项-自主创新项目（首发2020-2-8022）

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