目的 通过颈总动脉入路建立一种更具优势，且适用于缺血性脑卒中实施颈交感神经干离断研究的大脑中动脉闭塞（middle cerebral artery occlusion, MCAO）模型。 方法 将40只SD大鼠随机分为ECA组和CCA改良组，分别进行MCAO模型制备，观察两组的手术时间、模型成功率、Longa评分、梗死率及术后生存情况。然后将12只大鼠随机分为CCA组和CCA+CSB组，后者实施颈交感神经干离断，评估两组术后的Longa评分、梗死率以及BCL-2、BAX、Caspase-3的蛋白表达。 结果 与ECA组相比，CCA改良组有更高的模型成功率（P<0.05）、Longa评分（P<0.01）和梗死率（P<0.01）以及更短的手术总时间（P<0.001）；两组术后生存情况无明显差异。与CCA组相比，CCA+CSB组Longa评分下降（P<0.05），梗死率下降（P<0.05），且BCL-2的表达增加（P<0.01），而BAX（P<0.05）和Caspase-3（P<0.05）的表达均减少。 结论 与经典的ECA模型相比，CCA改良模型有显著的优势，有望成为缺血性脑卒中实施颈交感神经干离断研究的理想模型。
Abstract
Objective To establish a more advantageous and suitable middle cerebral artery occlusion (MCAO) model for the study of cervical sympathetic trunk transection in ischemic stroke through the common carotid artery (CCA) approach. Methods Forty rats were randomly allocated into the ECA group and the modified CCA group, and MCAO models were prepared in each group. The operative time, model success rate, Longa grade, infarct rate, and postoperative survival were assessed. Then twelve rats were randomly allocated into the CCA group and the CCA+CSB group. The latter group underwent cervical sympathetic nerve trunk transection. The Longa grade, infarct rate, and protein expression of BCL-2, BAX, and Caspase-3 were evaluated postoperatively in both groups. Results Compared with the ECA group, the modified CCA group showed higher model success rate (P<0.05), higher Longa score (P<0.01), and higher infarct rate (P<0.01), while shorter total operative time (P<0.001). There was no significant difference in postoperative survival between the two groups. Compared with the CCA group, the CCA+CSB group exhibited a lower Longa score (P<0.05) and lower infarct rate (P<0.05), increased BCL-2 expression (P<0.01), and decreased BAX (P<0.05) and Caspase-3 (P<0.05) expression. Conclusions The modified CCA model demonstrates significant advantages over the classic ECA model and is expected to serve as an ideal model for the study of cervical sympathetic trunk transection in ischemic stroke.
关键词
缺血性脑卒中 /
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颈总动脉入路 /
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大脑中动脉闭塞改良模型 /
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颈交感神经干离断
Key words
Ischemic stroke /
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Carotid artery approach /
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Middle cerebral artery occlusion modified model /
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Cervical sympathetic trunk transection
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基金
福建省自然科学基金联合资助项目（2024J011263）
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