黄芩苷对椎间盘退变模型大鼠的保护机制研究

doi:10.13418/j.issn.1001-165x.2026.1.09

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中国临床解剖学杂志 ›› 2026, Vol. 44 ›› Issue (1) : 53-60. DOI: 10.13418/j.issn.1001-165x.2026.1.09

实验研究

黄芩苷对椎间盘退变模型大鼠的保护机制研究

张伟^#，张杨^#，于英楠，张海斌，王鹏，吴一民*

作者信息 +

Study on the protective mechanism of Baicalin on intervertebral disc degeneration in rats

Zhang Wei ^#, Zhang Yang ^#, Yu Yingnan, Zhang Haibin , Wang Peng, Wu Yimin*

Author information +

文章历史 +

摘要

目的基于黄芩苷在骨关节炎等疾病中展现的抗炎与细胞外基质保护作用，探讨其对椎间盘退变的治疗潜力。方法 24只Sprague－Dawley大鼠随机分为假手术组、模型组及黄芩苷治疗组。采用免疫组化技术检测炎症因子和细胞外基质相关蛋白的表达；免疫组化和TUNEL染色评估椎间盘组织的凋亡情况；X射线、HE染色和番红固绿染色对椎间盘结构进行组织学分析。结果相较假手术组，模型组椎间盘结构出现退行性改变，炎症因子COX-2和iNOS表达升高，凋亡标志物BAX/Bcl-2比值升高，Cleaved Caspase-3活性升高，细胞外基质降解蛋白MMP-13和ADAMTS-5表达升高，细胞外基质合成蛋白COL-2和Aggrecan表达降低。而黄芩苷的加入可改善IDD退变程度，使炎症反应、细胞凋亡标志物、细胞外基质降解蛋白降低，细胞外基质合成蛋白升高。结论黄芩苷可通过抑制炎症反应、细胞凋亡及细胞外基质降解，缓解椎间盘退变大鼠模型的症状。

Abstract

Objective To investigate the therapeutic potential of Baicalein for intervertebral disc degeneration (IDD), based on the anti-inflammatory and extracellular matrix (ECM) protective effects of Baicalein in diseases such as osteoarthritis. Methods A total of 24 Sprague-Dawley (SD) rats were randomly divided into a sham-operated group, a model group, and a Baicalin-treated group. Immunohistochemistry was employed to detect the expression of inflammatory cytokines and ECM-related proteins. Apoptosis in intervertebral disc tissues was assessed via immunohistochemistry and TUNEL staining. Structural analysis of the intervertebral discs was performed using X-ray imaging, H&E staining, and Safranin O-Fast Green staining. Results Compared with the sham-operated group, the model group exhibited degenerative structural changes in intervertebral discs, elevated expression of inflammatory factors COX2 and iNOS, increased BAX/Bcl-2 ratio, heightened activity of Cleaved Caspase-3, upregulated expression of ECM-degrading protein MMP-13 and ADAMTS-5, and downregulated expression of ECM-synthesizing protein COL-2 and Aggrecan. Baicalin treatment ameliorated the degenerative changes in IDD, suppressed inflammatory responses, reduced apoptosis markers, decreased expression of ECM-degrading proteins, and increased expression of ECM-synthesizing proteins. Conclusions Baicalin alleviates IDD symptoms in a rat model by inhibiting inflammation, apoptosis, and extracellular matrix degradation.

导出引用

张伟, 张杨, 于英楠, 张海斌, 王鹏, 吴一民. 黄芩苷对椎间盘退变模型大鼠的保护机制研究[J]. 中国临床解剖学杂志. 2026, 44(1): 53-60 https://doi.org/10.13418/j.issn.1001-165x.2026.1.09

Zhang Wei , Zhang Yang, Yu Yingnan, Zhang Haibin , Wang Peng, Wu Yimin. Study on the protective mechanism of Baicalin on intervertebral disc degeneration in rats[J]. Chinese Journal of Clinical Anatomy. 2026, 44(1): 53-60 https://doi.org/10.13418/j.issn.1001-165x.2026.1.09

中图分类号： R681.5

参考文献

[1] Xu H, Li J, Fei Q, et al. Contribution of immune cells to intervertebral disc degeneration and the potential of immunotherapy [J]. Connect Tissue Res,2023,64(5): 413-427. DOI: 10.1080/03008207. 2023. 2212051.
[2] Kirnaz S, Capadona C, Wong T, et al. Fundamentals of Intervertebral Disc Degeneration [J]. World Neurosurg, 2022, 157: 264-273. DOI: 10.1016/j.wneu.2021.09.066.
[3] Chen X, Lu S, Chopra N, et al. The association between low virulence organisms in different locations and intervertebral disc structural failure: A meta-analysis and systematic review [J]. JOR Spine, 2023, 6(2): e1244. DOI: 10.1002/jsp2.1244.
[4] Pan J, Liu Z, Shen B, et al. tsRNA-04002 alleviates intervertebral disk degeneration by targeting PRKCA to inhibit apoptosis of nucleus pulposus cells [J]. J Orthop Surg Res, 2023, 18(1): 413. DOI: 10.1186/s13018-023-03878-3.
[5] Liu MY, Zhang L, Zang WD, et al. Pharmacological Effects of Resveratrol in Intervertebral Disc Degeneration: A Literature Review [J]. Orthop Surg, 2022, 14(12): 3141-3149. DOI: 10.1111/os.13560.
[6] Deml MC, Benneker LM, Schmid T, et al. Ventral Surgical Approach for an Intervertebral Disc Degeneration and Regeneration Model in Sheep Cervical Spine: Anatomic Technical Description, Strengths and Limitations [J]. Vet Comp Orthop Traumatol, 2019, 32(5): 389-393. DOI: 10.1055/s-0039-1688988.
[7] Eisenstein SM, Balain B, Roberts S. Current Treatment Options for Intervertebral Disc Pathologies [J]. Cartilage, 2020, 11(2): 143-151. DOI: 10.1177/1947603520907665.
[8] Xin J, Wang Y, Zheng Z, et al. Treatment of Intervertebral Disc Degeneration [J]. Orthop Surg, 2022, 14(7): 1271-1280. DOI:10.1111/os.13254.
[9] Fan N, Yuan S, Du P, et al. Complications and risk factors of percutaneous endoscopic transforaminal discectomy in the treatment of lumbar spinal stenosis [J]. BMC Musculoskelet Disord, 2021, 22(1): 1041. DOI: 10.1186/s12891-021-04940-z.
[10]Ju CI, Lee SM. Complications and Management of Endoscopic Spinal Surgery[J]. Neurospine, 2023, 20(1): 56-77.DOI:10.14245/ns.2346 226.113.
[11]Bajek-Bil A, Chmiel M, Włoch A, et al. Baicalin-Current Trends in Detection Methods and Health-Promoting Properties [J]. Pharmaceuticals (Basel, Switzerland), 2023, 16(4): 570. DOI: 10.3390/ph16040570.
[12]Wen Y, Wang Y, Zhao C, et al. The Pharmacological Efficacy of Baicalin in Inflammatory Diseases [J]. Int J Mol Sci, 2023, 24(11): 9317. DOI: 10.3390/ijms24119317.
[13]Singh S, Meena A, Luqman S. Baicalin mediated regulation of key signaling pathways in cancer [J]. Pharmacol Res, 2021, 164: 105387. DOI: 10.1016/j.phrs.2020.105387.
[14]Hu Q, Zhang W, Wu Z, et al. Baicalin and the liver-gut system: Pharmacological bases explaining its therapeutic effects [J]. Pharmacol Res, 2021, 165: 105444. DOI: 10.1016/j.phrs.2021.105444.
[15]Lu L, Rao L, Jia H, et al. Baicalin positively regulates osteoclast function by activating MAPK/Mitf signalling [J]. J Cell Mol Med, 2017, 21(7): 1361-1372. DOI: 10.1111/jcmm.13066.
[16]Wang B, Huang T, Fang Q, et al. Bone-protective and anti-tumor effect of baicalin in osteotropic breast cancer via induction of apoptosis [J]. Breast Cancer Res Treat, 2020, 184(3): 711-721. DOI: 10.1007/s10549-020-05904-y.
[17]Lu X, He W, Yang W, et al. Dual effects of baicalin on osteoclast differentiation and bone resorption [J]. J Cell Mol Med, 2018, 22(10): 5029-5039. DOI: 10.1111/jcmm.13785.
[18]Wu T, Weng Z, Xu J, et al. Baicalin alleviates osteomyelitis by regulating TLR2 in the murine model [J]. Pathog Dis, 2018, 76(2). DOI: 10.1093/femspd/ftx123.
[19]Iqbal M, Waqas M, Mo Q, et al. Baicalin inhibits apoptosis and enhances chondrocyte proliferation in thiram-induced tibial dyschondroplasia in chickens by regulating Bcl-2/Caspase-9 and Sox-9/Collagen-II expressions [J]. Ecotoxicol Environ Saf, 2023, 268: 115689. DOI: 10.1016/j.ecoenv.2023.115689.
[20]Zhao Y, Wang HL, Li TT, et al. Baicalin Ameliorates Dexamethasone-Induced Osteoporosis by Regulation of the RANK/RANKL/OPG Signaling Pathway [J]. Drug Desi Devel Ther, 2020, 14: 195-206. DOI: 10.2147/dddt.S225516.
[21]Wang F, Cai F, Shi R, et al. Aging and age related stresses: a senescence mechanism of intervertebral disc degeneration [J]. Osteoarthritis Cartilage, 2016, 24(3): 398-408. DOI: 10.1016/j.joca.2015.09.019.
[22]Samanta A, Lufkin T, Kraus P. Intervertebral disc degeneration-Current therapeutic options and challenges [J]. Front Public Health, 2023, 11: 1156749. DOI: 10.3389/fpubh.2023.1156749.
[23]Huang X, He Y, Chen Y, et al. Baicalin attenuates bleomycin-induced pulmonary fibrosis via adenosine A2a receptor related TGF-β1-induced ERK1/2 signaling pathway [J]. BMC Pulm Med, 2016, 16(1): 132. DOI: 10.1186/s12890-016-0294-1.
[24]Zhao D, Dou YX, Zeng LF, et al. The effects of extracellular matrix-degrading enzymes polymorphisms on intervertebral disc degeneration [J]. JOR Spine, 2024, 7(4): e70012. DOI: 10.1002/jsp2.70012.
[25]Jiang C, Liu Y, Zhao W, et al. microRNA-365 attenuated intervertebral disc degeneration through modulating nucleus pulposus cell apoptosis and extracellular matrix degradation by targeting EFNA3 [J]. J Cell Mol Med, 2024, 28(2): e18054. DOI: 10.1111/jcmm.18054.
[26]Kepler CK, Ponnappan RK, Tannoury CA, et al. The molecular basis of intervertebral disc degeneration [J]. Spine J, 2013, 13(3): 318-330. DOI: 10.1016/j.spinee.2012.12.003.
[27]Liang H, Luo R, Li G, et al. The Proteolysis of ECM in Intervertebral Disc Degeneration [J]. Int J Mol Sci, 2022, 23(3): 1715. DOI: 10.3390/ijms23031715.
[28]Xiao Y, Ye J, Zhou Y, et al. Baicalin inhibits pressure overload-induced cardiac fibrosis through regulating AMPK/TGF-β/Smads signaling pathway [J]. Arch Biochem Biophys, 2018, 640: 37-46. DOI: 10.1016/j.abb.2018.01.006.
[29]Li Z, Cheng J, Liu J. Baicalin Protects Human OA Chondrocytes Against IL-1β-Induced Apoptosis and ECM Degradation by Activating Autophagy via MiR-766-3p/AIFM1 Axis [J]. Drug Des Devel Ther, 2020, 14: 2645-2655. DOI: 10.2147/dddt.S255823.
[30]Liu J, Zhou H, Chen J, et al. Baicalin inhibits IL-1β-induced ferroptosis in human osteoarthritis chondrocytes by activating Nrf-2 signaling pathway [J]. J Orthop Surg Res, 2024, 19(1): 23. DOI: 10.1186/s13018-023-04483-0.
[31] Yang SD, Bai ZL, Zhang F, et al. Levofloxacin increases the effect of serum deprivation on anoikis of rat nucleus pulposus cells via Bax/Bcl-2/caspase-3 pathway [J]. Toxicol Mech Methods, 2014, 24(9): 688-696. DOI: 10.3109/15376516.2014.963772.
[32] Bai X, Wang J, Ding S, et al. Embelin protects against apoptosis and inflammation by regulating PI3K/Akt signaling in IL-1β-stimulated human nucleus pulposus cells [J]. Tissue Cell, 2023, 82: 102089. DOI: 10.1016/j.tice.2023.102089.
[33] Tao C, Lin S, Shi Y, et al. Inactivation of Tnf-α/Tnfr signaling attenuates progression of intervertebral disc degeneration in mice [J]. JOR Spine, 2024, 7(4): e70006. DOI: 10.1002/jsp2.70006.
[34] Yang H, Chen X, Chen J, et al. The pathogenesis and targeted therapies of intervertebral disc degeneration induced by cartilage endplate inflammation [J]. Front Cell Dev Biol, 2024, 12: 1492870. DOI: 10.3389/fcell.2024.1492870.