目的 分析天麻破壁粉基于环腺苷酸应答元件结合蛋白/脑源性神经营养因子（CREB/BDNF）信号通路对癫痫大鼠的干预效果。 方法 选取SPF级SD雄性大鼠40只（建模过程死亡2只），其中对照组10只，模型组12只，药物对比组8只，天麻破壁粉组8只。对照组、模型组仅进行生理盐水灌胃，不做其他处理。药物对比组给予吡仑帕奈0.3 g/kg水溶液灌胃，天麻破壁粉组给予天麻破壁粉1.0 g/kg灌胃。评估大鼠自身行为；记录癫痫持续时间；观察细胞凋亡率；检测TNF-α、IL-1β、IL-6、CAT、MDA、SOD水平；评估大鼠记忆能力；检测CREB/BDNF信号通路相关蛋白。 结果 相较对照组，模型组、药物对比组、天麻破壁粉组行为学评分水平、细胞凋亡率情况、TNF-α、IL-1β、IL-6、MDA水平、逃避潜伏期时间均升高（P<0.05），CAT、SOD水平、穿越平台次数情况、p-CREB/CREB、BDNF蛋白表达量均降低（P<0.05）；相较模型组、药物对比组，天麻破壁粉组行为学评分水平、细胞凋亡率、发作持续时间、TNF-α、IL-1β、IL-6、MDA水平、逃避潜伏期时间均降低（P<0.05），CAT、SOD水平、穿越平台次数、p-CREB/CREB、BDNF蛋白表达均升高（P<0.05）。 结论 天麻破壁粉上调CREB/BDNF通路活性，可改善癫痫大鼠记忆能力，抑制细胞凋亡率，缓解神经炎症、抑制氧化应激状态。
Abstract
Objective To analyze the intervention effect of gastrodia elata wall breaking powder in epileptic rats based on cyclic adenylate response element binding protein / brain-derived neurotrophic factor (CREB/BDNF) signaling pathway. Methods 38 SD male rats were divided into the control group (10), the model group (12), the drug control group (8) and Gastrodia elata wall powder group (8), respectively. The control and model groups were gavaged with saline. In the drug control group, 0.3 g/kg aqueous solution of piranpanet was given for gavage, and in the Gastrodia elata wall powder group, 1.0 g/kg powder solution was given. The behavior of rats was assessed. The duration of epilepsy was recorded. The rate of cell apoptotic was observed. The levels of TNF- α, IL-1 β, IL-6, CAT, MDA and SOD were measured. The memory ability of rats was assessed, and the levels of CREB / BDNF signaling pathway proteins were detected. Results Compared with the control group, the behavioral scores, cell apoptosis rate, TNF-α, IL-1 β, IL-6 and MDA level and the escape latency time in the model, drug control and Gastrodia elata wall powder groups were markedly increased (P<0.05). However, CAT and SOD levels, the number of platform crossing, p-CREB / CREB, and BDNF protein expression were all decreased (P<0.05). Compared with the model and the drug-control group, , the behavioral score, apoptosis rate, duration of attack, TNF- α, IL-1 β, IL-6, MDA level, and escape latency time in the Gastrodia elata wall powder group were all decreased (P<0.05), but CAT and SOD levels, times of platform crossing, p-CREB/CREB, and BDNF protein expression were all increased (P<0.05). Conclusions The wall-breaking powder of Gastrodia elata increases the activity of CREB/BDNF pathway and improves the memory ability of epileptic rats, inhibits the cell apoptosis rate, alleviates neuroinflammation and inhibits oxidative stress state.
关键词
天麻破壁粉 /
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环腺苷酸应答元件结合蛋白/脑源性神经营养因子信号 /
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癫痫 /
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大鼠
Key words
Gastrodia elata wall-breaking powder /
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Cyclic adenylate response element binding protein / Brain-derived neurotrophic factor signaling pathway /
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Epilepsy /
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Rat
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基金
广东省东莞市社会发展科技项目（20231800903722）；广东省中医药局科研项目（20181264）
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