外泌体复合脱细胞真皮基质水凝胶在创面修复中作用的实验研究#br#

doi:10.13418/j.issn.1001-165x.2025.5.10

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中国临床解剖学杂志 ›› 2025, Vol. 43 ›› Issue (5) : 564-572. DOI: 10.13418/j.issn.1001-165x.2025.5.10

实验研究

外泌体复合脱细胞真皮基质水凝胶在创面修复中作用的实验研究#br#

任力¹，王芳²，彭倩³，杨智勇⁴*

作者信息 +

Experimental study on the effect of exosome combined acellular dermal matrix hydrogel on wound repair

Ren Li¹, Wang Fang², Peng Qian³, Yang Zhiyong⁴*

Author information +

文章历史 +

摘要

目的探究外泌体复合脱细胞真皮基质水凝胶对大鼠皮肤创面愈合的影响及作用机制。方法取第4~6代大鼠皮肤成纤维细胞，分4组处理：PBS组、水凝胶组、外泌体组、外泌体-水凝胶组，CCK-8检测细胞增殖，划痕实验检测细胞迁移，RT-qPCR检测Ⅰ型胶原、Ⅲ型胶原、血管内皮生长因子、α-平滑肌肌动蛋白的mRNA表达。建立SD大鼠背部全层皮肤缺损创面，将大鼠分为空白对照组、水凝胶组、外泌体组、外泌体-水凝胶组，观察创面愈合情况，HE染色与Masson染色观察创面组织病理变化，Western blot检测创面组织Ⅰ型胶原、Ⅲ型胶原、血管内皮生长因子、α-平滑肌肌动蛋白的蛋白表达。结果与PBS组相比，外泌体组比较，外泌体-水凝胶组成纤维细胞增殖与迁移能力显著增强（P<0.05），Ⅰ型胶原、Ⅲ型胶原、血管内皮生长因子、α-平滑肌肌动蛋白的mRNA表达量升高（P<0.05）。与外泌体组相比，外泌体-水凝胶组成纤维细胞增殖与迁移能力显著增强（P<0.05），Ⅰ型胶原、Ⅲ型胶原、血管内皮生长因子、α-平滑肌肌动蛋白的mRNA表达量升高（P<0.05）。与空白对照组相比，水凝胶组、外泌体组、外泌体-水凝胶组创面新生上皮组织完整，新生胶原密度大，创面愈合更快，Ⅰ型胶原、Ⅲ型胶原、血管内皮生长因子、α-平滑肌肌动蛋白的蛋白表达升高（P<0.05）。与水凝胶组、外泌体组相比，外泌体-水凝胶组创面厚度增大，胶原分布更加均匀，新生上皮创面愈合更快，Ⅰ型胶原、Ⅲ型胶原、血管内皮生长因子、α-平滑肌肌动蛋白的蛋白表达升高（P<0.05）。结论外泌体-脱细胞真皮基质水凝胶可通过促进成纤维细胞的增殖、迁移及分泌Ⅰ型胶原、Ⅲ型胶原、血管内皮生长因子、α-平滑肌肌动蛋白等因子表达来促进创面的修复。

Abstract

Objective To explore the effect and mechanism of exosome combined with acellular dermal matrix hydrogel on skin wound healing in rats. Methods The 4th~6th generation of rat skin fibroblasts were divided into 4 groups: PBS group, hydrogel group, exosome group, and exosome hydrogel group. CCK-8 was used to detect cell proliferation, scratch assay was used to detect cell migration, and RT-qPCR was used to detect mRNA expression of type I collagen, type III collagen, vascular endothelial growth factor, and α-smooth muscle actin. The full-thickness skin defect wounds of SD rats were established, and the rats were divided into blank control group, hydrogel group, exosome group and exosome hydrogel group. The wound healing rate was observed, and the histopathological changes were observed by HE staining and Masson staining. The protein expressions of type I collagen, type III collagen, vascular endothelial growth factor and α-smooth muscle actin were detected by Western blot. Results Compared with PBS group and exosome group, the proliferation and migration ability of exosome hydrogel composed fibrocytes were significantly enhanced (P<0.05), and the mRNA expression levels of type I collagen, type III collagen, vascular endothelial growth factor and α-smooth muscle actin were increased (P<0.05). Compared with the exosome group, the proliferation and migration of exosome hydrogel fibrocytes were significantly enhanced (P<0.05), and the mRNA expressions of type I collagen, type III collagen, vascular endothelial growth factor and α-smooth muscle actin were increased (P<0.05).Compared with the blank control group, the neoepithelial tissue of the wound in hydrogel group, exosome group and exosome hydrogel group was intact, the density of new collagen was higher, the wound healing was faster, and the protein expressions of type I collagen, type III collagen, vascular endothelial growth factor and α-smooth muscle actin were increased (P<0.05). Compared with the hydrogel group and the exosome group, the wound thickness of the exosome hydrogel group was increased, the collagen distribution was more uniform, the neoepithelial wound healing was faster, and the protein expressions of type I collagen, type III collagen, vascular endothelial growth factor and α-smooth muscle actin were increased in the exosome hydrogel group (P<0.05). Conclusions Exosome acellular dermal matrix hydrogel can promote wound repair by promoting the proliferation and migration of fibroblasts and the expression of type I collagen, type III collagen, vascular endothelial growth factor, α-smooth muscle actin and other factors.

导出引用

任力, 王芳, 彭倩, 杨智勇. 外泌体复合脱细胞真皮基质水凝胶在创面修复中作用的实验研究#br#[J]. 中国临床解剖学杂志. 2025, 43(5): 564-572 https://doi.org/10.13418/j.issn.1001-165x.2025.5.10

Ren Li, Wang Fang, Peng Qian, Yang Zhiyong. Experimental study on the effect of exosome combined acellular dermal matrix hydrogel on wound repair[J]. Chinese Journal of Clinical Anatomy. 2025, 43(5): 564-572 https://doi.org/10.13418/j.issn.1001-165x.2025.5.10

中图分类号： R-33 R628

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