防风多糖药物血清对神经元兴奋性损伤的保护作用及递质释放的影响

doi:10.13418/j.issn.1001-165x.2025.5.08

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中国临床解剖学杂志 ›› 2025, Vol. 43 ›› Issue (5) : 548-554. DOI: 10.13418/j.issn.1001-165x.2025.5.08

实验研究

防风多糖药物血清对神经元兴奋性损伤的保护作用及递质释放的影响

张丹¹，杨晓茜²，宋瑞³，孙晋浩³，徐涛²*，李文杰²*

作者信息 +

The Protective Effect of Saposhnikovia divaricata Polysaccharide-Containing Serum on Neuronal Excitotoxicity and Neurotransmitter Release in PC12 Cells

Zhang Dan¹, Yang Xiaoqian², Song Rui³, Sun Jinhao³, Xu Tao²*, Li Wenjie²*

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文章历史 +

摘要

目的探讨防风多糖（SP）对谷氨酸（Glu）诱导的PC12细胞兴奋性损伤的神经保护作用及对儿茶酚胺（CA）类递质储存和释放的影响。方法给予15 mM Glu 处理PC12细胞，建立神经元兴奋性损伤细胞模型；经SD大鼠灌服SP制备含药血清并加入至PC12细胞培养液内。通过MTT和DCFH-DA探针分别检测SP药物血清对PC12细胞活性和活性氧（ROS）水平的影响。采用单细胞安培法（SCA）和胞内囊泡碰撞的电化学方法（IVIEC）检测SP药物血清对PC12细胞单囊泡CA储存、分泌及胞吐动力学的影响；经透射电镜观察囊泡超微结构的变化。结果 SP含药血清显著提高Glu损伤PC12细胞后的细胞活性；降低ROS含量；并在单个囊泡水平上增加CA的储存和和释放，增大囊泡及其致密核心体积。结论 SP通过减轻氧化应激发挥对Glu兴奋性损伤的神经保护作用，并通过上调单囊泡内CA的储存和释放，调控CA类神经递质释放稳态。

Abstract

Objective To investigate the neuroprotection of Saposhnikovia divaricata polysaccharides (SP) against glutamate (Glu)-induced excitotoxic injury and catecholamine (CA) neurotransmitters release in PC12 cells. Methods The cultured PC12 cells were treated with 15 mM Glu and the excitotoxicity injury model was established. SP-containing serum was prepared from rats administered with SP and added in culture plates. Cell viability was assessed via MTT assay. Intracellular ROS levels were detected with DCFH-DA probe. The effects of SP-containing serum on the single-vesicle CA storage, secretion, and exocytosis kinetics were detected using single-cell amperometry (SCA) and intracellular vesicle impact electrochemical cytometry (IVIEC). Vesicle ultrastructure was observed via transmission electron microscopy (TEM). Results SP-containing serum significantly improved the viability of Glu-damaged cells, reduced ROS content, increased CA storage and release at the single-vesicle level, and reversed the Glu - induced reduction in vesicle and dense core volumes. Conclusions SP exhibits obvious neuroprotective effect by alleviating oxidative stress, and SP can regulate CA release homeostasis by enhancing storage and release.

导出引用

张丹, 杨晓茜, 宋瑞, 孙晋浩, 徐涛, 李文杰. 防风多糖药物血清对神经元兴奋性损伤的保护作用及递质释放的影响[J]. 中国临床解剖学杂志. 2025, 43(5): 548-554 https://doi.org/10.13418/j.issn.1001-165x.2025.5.08

Zhang Dan, Yang Xiaoqian, Song Rui, Sun Jinhao, Xu Tao, Li Wenjie. The Protective Effect of Saposhnikovia divaricata Polysaccharide-Containing Serum on Neuronal Excitotoxicity and Neurotransmitter Release in PC12 Cells[J]. Chinese Journal of Clinical Anatomy. 2025, 43(5): 548-554 https://doi.org/10.13418/j.issn.1001-165x.2025.5.08

中图分类号： R285 O657.1

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