针灸对脊髓损伤大鼠血清Leptin水平及神经元活性的影响

doi:10.13418/j.issn.1001-165x.2025.4.14

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中国临床解剖学杂志 ›› 2025, Vol. 43 ›› Issue (4) : 450-455. DOI: 10.13418/j.issn.1001-165x.2025.4.14

实验研究

针灸对脊髓损伤大鼠血清Leptin水平及神经元活性的影响

周斌，李占涛，左艳丽*

作者信息 +

Effects of acupuncture on serum Leptin level and neuronal activity in rats with spinal cord injury

Zhou Bin, Li Zhantao, Zuo Yanli*

Author information +

文章历史 +

摘要

目的基于PI3K /Akt信号通路探讨针灸对脊髓损伤大鼠血清Leptin水平及神经元活性的机制。方法 30只SPF级SD大鼠按随机法分为Sham组、模型组及针灸组，均10只。除Sham组外均建立脊髓损伤（SCI）大鼠模型，建模成功后，针灸组进行针灸治疗，其余两组不干预。结果与模型组相比，针灸组大鼠神经功能评分降低（P<0.05）；与Sham组相比，模型组大鼠血清Leptin水平升高（P<0.05），与模型组相比，针灸组大鼠血清Leptin水平更高（P<0.05）； Sham组、模型组及针灸组的大鼠神经细胞凋亡率分别为（4.20±0.69）%、（26.81±4.59）%及（18.41±3.54）%，差异有显著性（F=115.000，P<0.001）；与Sham组相比，模型组大鼠神经细胞PI3K、p-PI3K、AKT及p-AKT蛋白水平降低（P<0.05），与模型组相比，针灸组大鼠PI3K、p-PI3K、AKT及p-AKT蛋白水平升高（P<0.05）。结论针灸可减少脊髓损伤大鼠神经细胞凋亡、改善神经功能，同时可上调Leptin水平以改善脊髓损伤，可能与调节PI3K /Akt活性相关。

Abstract

Objective To explore the mechanism of acupuncture on serum Leptin level and neuronal activity in rats with spinal cord injury based on PI3K /Akt signaling pathway. Methods Thirty SD rats were randomly divided into Sham group, model group and acupuncture group, with 10 rats in each group. Spinal cord injury (SCI) rat models were established in all groups except Sham group. After successful modeling, acupuncture treatment was performed in the acupuncture group, while no intervention for other 2 groups. Results Compared with model group, nerve function scores of rats in acupuncture group was decreased (P<0.05). Compared with the Sham group, the serum Leptin level of rats in model group increased (P<0.05), and the serum Leptin level of rats in acupuncture group was higher than that in the model group (P<0.05). The apoptosis rates of neurons in Sham, model and acupuncture groups were (4.20±0.69) %, (26.81±4.59) % and (18.41±3.54) %, respectively, with statistically significant difference (F=115.000, P<0.001). Compared with Sham group, the levels of PI3K, p-PI3K, AKT and p-Akt protein in neurons of rats in model group decreased (P<0.05). Compared with model group, the levels of PI3K, p-PI3K, AKT and p-Akt increased in acupuncture group (P<0.05). Conclusions Acupuncture can reduce neuron apoptosis and improve nerve function in SCI rats. Acupuncture can up-regulate Leptin level and improve the repair of spinal cord injury, which may be related to the regulation of PI3K /Akt activity.

导出引用

周斌, 李占涛, 左艳丽. 针灸对脊髓损伤大鼠血清Leptin水平及神经元活性的影响[J]. 中国临床解剖学杂志. 2025, 43(4): 450-455 https://doi.org/10.13418/j.issn.1001-165x.2025.4.14

Zhou Bin, Li Zhantao, Zuo Yanli. Effects of acupuncture on serum Leptin level and neuronal activity in rats with spinal cord injury[J]. Chinese Journal of Clinical Anatomy. 2025, 43(4): 450-455 https://doi.org/10.13418/j.issn.1001-165x.2025.4.14

中图分类号： R681

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